How to correctly estimate the electric field in capacitively coupled systems for tissue engineering: a comparative study
Autor(a) principal: | |
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Data de Publicação: | 2022 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10400.8/8157 |
Resumo: | Capacitively Coupled (CCoupled) electric fields are used to stimulate cell cultures in Tissue Engineering. Knowing the electric field (E-Field) magnitude in the culture medium is fundamental to establish a relationship between stimulus strength and cellular effects. We analysed eight CCoupled studies and sought to corroborate the reported estimates of the E-Field in the culture medium. First, we reviewed the basic physics underlying CCoupled stimulation and delineated three approaches to estimate the E-field. Using these approaches, we found that the reported values were overestimated in five studies, four of which were based on incorrect assumptions. In all studies, insufficient information was provided to reproduce the setup exactly. Creating electrical models of the experimental setup should improve the accuracy of the E-field estimates and enhance reproducibility. For this purpose, we developed a free open-source tool, the E-field Calculator for CCoupled systems, which is available for download from an internet hosting platform. |
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How to correctly estimate the electric field in capacitively coupled systems for tissue engineering: a comparative studyCapacitively Coupled (CCoupled) electric fields are used to stimulate cell cultures in Tissue Engineering. Knowing the electric field (E-Field) magnitude in the culture medium is fundamental to establish a relationship between stimulus strength and cellular effects. We analysed eight CCoupled studies and sought to corroborate the reported estimates of the E-Field in the culture medium. First, we reviewed the basic physics underlying CCoupled stimulation and delineated three approaches to estimate the E-field. Using these approaches, we found that the reported values were overestimated in five studies, four of which were based on incorrect assumptions. In all studies, insufficient information was provided to reproduce the setup exactly. Creating electrical models of the experimental setup should improve the accuracy of the E-field estimates and enhance reproducibility. For this purpose, we developed a free open-source tool, the E-field Calculator for CCoupled systems, which is available for download from an internet hosting platform.NatureIC-OnlineMeneses, JoãoFernandes, SofiaAlves, NunoPascoal-Faria, PaulaMiranda, Pedro Cavaleiro2023-02-23T15:26:43Z20222022-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10400.8/8157engMeneses, J., Fernandes, S., Alves, N. et al. How to correctly estimate the electric field in capacitively coupled systems for tissue engineering: a comparative study. Sci Rep 12, 11049 (2022). https://doi.org/10.1038/s41598-022-14834-22045-232210.1038/s41598-022-14834-2info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-01-17T15:56:39Zoai:iconline.ipleiria.pt:10400.8/8157Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T01:50:55.275328Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
How to correctly estimate the electric field in capacitively coupled systems for tissue engineering: a comparative study |
title |
How to correctly estimate the electric field in capacitively coupled systems for tissue engineering: a comparative study |
spellingShingle |
How to correctly estimate the electric field in capacitively coupled systems for tissue engineering: a comparative study Meneses, João |
title_short |
How to correctly estimate the electric field in capacitively coupled systems for tissue engineering: a comparative study |
title_full |
How to correctly estimate the electric field in capacitively coupled systems for tissue engineering: a comparative study |
title_fullStr |
How to correctly estimate the electric field in capacitively coupled systems for tissue engineering: a comparative study |
title_full_unstemmed |
How to correctly estimate the electric field in capacitively coupled systems for tissue engineering: a comparative study |
title_sort |
How to correctly estimate the electric field in capacitively coupled systems for tissue engineering: a comparative study |
author |
Meneses, João |
author_facet |
Meneses, João Fernandes, Sofia Alves, Nuno Pascoal-Faria, Paula Miranda, Pedro Cavaleiro |
author_role |
author |
author2 |
Fernandes, Sofia Alves, Nuno Pascoal-Faria, Paula Miranda, Pedro Cavaleiro |
author2_role |
author author author author |
dc.contributor.none.fl_str_mv |
IC-Online |
dc.contributor.author.fl_str_mv |
Meneses, João Fernandes, Sofia Alves, Nuno Pascoal-Faria, Paula Miranda, Pedro Cavaleiro |
description |
Capacitively Coupled (CCoupled) electric fields are used to stimulate cell cultures in Tissue Engineering. Knowing the electric field (E-Field) magnitude in the culture medium is fundamental to establish a relationship between stimulus strength and cellular effects. We analysed eight CCoupled studies and sought to corroborate the reported estimates of the E-Field in the culture medium. First, we reviewed the basic physics underlying CCoupled stimulation and delineated three approaches to estimate the E-field. Using these approaches, we found that the reported values were overestimated in five studies, four of which were based on incorrect assumptions. In all studies, insufficient information was provided to reproduce the setup exactly. Creating electrical models of the experimental setup should improve the accuracy of the E-field estimates and enhance reproducibility. For this purpose, we developed a free open-source tool, the E-field Calculator for CCoupled systems, which is available for download from an internet hosting platform. |
publishDate |
2022 |
dc.date.none.fl_str_mv |
2022 2022-01-01T00:00:00Z 2023-02-23T15:26:43Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10400.8/8157 |
url |
http://hdl.handle.net/10400.8/8157 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Meneses, J., Fernandes, S., Alves, N. et al. How to correctly estimate the electric field in capacitively coupled systems for tissue engineering: a comparative study. Sci Rep 12, 11049 (2022). https://doi.org/10.1038/s41598-022-14834-2 2045-2322 10.1038/s41598-022-14834-2 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Nature |
publisher.none.fl_str_mv |
Nature |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
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Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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RCAAP |
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RCAAP |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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