Genome-wide sequencing and metabolic annotation of Pythium irregulare CBS 494.86: understanding Eicosapentaenoic acid production

• Autor(a) principal: Fernandes, B. S.
• Data de Publicação: 2019
• Outros Autores: Dias, Oscar, Costa, Gisela, Kaupert Neto, Antonio A., Resende, Tiago F., Oliveira, Juliana V. C., Riaño-Pachón, Diego M., Zaiat, Marcelo, Pradella, José G. C., Rocha, I.
• Tipo de documento: Artigo
• Idioma: eng
• Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
• Texto Completo: http://hdl.handle.net/1822/60757
• topic: Eicosapentaenoic acid; Metabolic annotation; Pythium irregulare. unsaturated fatty acids, whole-genome sequence; Pythium irregulare; unsaturated fatty acids; whole-genome sequence; Science & Technology

Pythium irregulare is an oleaginous Oomycete able to accumulate large amounts of lipids, including Eicosapentaenoic acid (EPA). EPA is an important and expensive dietary supplement with a promising and very competitive market, which is dependent on fish-oil extraction. This has prompted several research groups to study biotechnological routes to obtain specific fatty acids rather than a mixture of various lipids. Moreover, microorganisms can use low cost carbon sources for lipid production, thus reducing production costs. Previous studies have highlighted the production of EPA by P. irregulare, exploiting diverse low cost carbon sources that are produced in large amounts, such as vinasse, glycerol, and food wastewater. However, there is still a lack of knowledge about its biosynthetic pathways, because no functional annotation of any Pythium sp. exists yet. The goal of this work was to identify key genes and pathways related to EPA biosynthesis, in P. irregulare CBS 494.86, by sequencing and performing an unprecedented annotation of its genome, considering the possibility of using wastewater as a carbon source.