Genome-wide sequencing and metabolic annotation of Pythium irregulare CBS 494.86: understanding Eicosapentaenoic acid production

Detalhes bibliográficos
Autor(a) principal: Fernandes, B. S.
Data de Publicação: 2019
Outros Autores: Dias, Oscar, Costa, Gisela, Kaupert Neto, Antonio A., Resende, Tiago F., Oliveira, Juliana V. C., Riaño-Pachón, Diego M., Zaiat, Marcelo, Pradella, José G. C., Rocha, I.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/1822/60757
Resumo: Pythium irregulare is an oleaginous Oomycete able to accumulate large amounts of lipids, including Eicosapentaenoic acid (EPA). EPA is an important and expensive dietary supplement with a promising and very competitive market, which is dependent on fish-oil extraction. This has prompted several research groups to study biotechnological routes to obtain specific fatty acids rather than a mixture of various lipids. Moreover, microorganisms can use low cost carbon sources for lipid production, thus reducing production costs. Previous studies have highlighted the production of EPA by P. irregulare, exploiting diverse low cost carbon sources that are produced in large amounts, such as vinasse, glycerol, and food wastewater. However, there is still a lack of knowledge about its biosynthetic pathways, because no functional annotation of any Pythium sp. exists yet. The goal of this work was to identify key genes and pathways related to EPA biosynthesis, in P. irregulare CBS 494.86, by sequencing and performing an unprecedented annotation of its genome, considering the possibility of using wastewater as a carbon source.
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spelling Genome-wide sequencing and metabolic annotation of Pythium irregulare CBS 494.86: understanding Eicosapentaenoic acid productionEicosapentaenoic acidMetabolic annotationPythium irregulare. unsaturated fatty acids, whole-genome sequencePythium irregulareunsaturated fatty acidswhole-genome sequenceScience & TechnologyPythium irregulare is an oleaginous Oomycete able to accumulate large amounts of lipids, including Eicosapentaenoic acid (EPA). EPA is an important and expensive dietary supplement with a promising and very competitive market, which is dependent on fish-oil extraction. This has prompted several research groups to study biotechnological routes to obtain specific fatty acids rather than a mixture of various lipids. Moreover, microorganisms can use low cost carbon sources for lipid production, thus reducing production costs. Previous studies have highlighted the production of EPA by P. irregulare, exploiting diverse low cost carbon sources that are produced in large amounts, such as vinasse, glycerol, and food wastewater. However, there is still a lack of knowledge about its biosynthetic pathways, because no functional annotation of any Pythium sp. exists yet. The goal of this work was to identify key genes and pathways related to EPA biosynthesis, in P. irregulare CBS 494.86, by sequencing and performing an unprecedented annotation of its genome, considering the possibility of using wastewater as a carbon source.The genome sequencing, strain acquisition and preliminary experiments and data analysis were funded by the São Paulo Research Foundation (FAPESP) and Coordination for the Improvement of Higher Education Personnel (CAPES) (Grante: 2016/10562–4). The experiment performance, data collection, analysis and interpretation of data were supported by the Portuguese Foundation for Science and Technology (FCT) under the scope of the strategic funding of [UID/BIO/04469] unit and COMPETE 2020 [POCI01-0145-FEDER-006684] and BioTecNorte operation [NORTE-01-0145-FEDER000004] funded by the European Regional Development Fund under the scope of Norte2020 - Programa Operacional Regional do Norte. The authors thank the project DD-DeCaF - Bioinformatics Services for Data-Driven Design of Cell Factories and Communities, Ref. H2020-LEIT-BIO-2015-1 686070–1, funded by the European Commission.info:eu-repo/semantics/publishedVersionSpringer NatureUniversidade do MinhoFernandes, B. S.Dias, OscarCosta, GiselaKaupert Neto, Antonio A.Resende, Tiago F.Oliveira, Juliana V. C.Riaño-Pachón, Diego M.Zaiat, MarceloPradella, José G. C.Rocha, I.2019-122019-12-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/1822/60757engFernandes, B. S.; Dias, Oscar; Costa, Gisela; Kaupert Neto, Antonio A.; Resende, Tiago F.; Oliveira, Juliana V. C.; Riaño-Pachón, Diego M.; Zaiat, Marcelo; Pradella, José G. C.; Rocha, Isabel, Genome-wide sequencing and metabolic annotation of Pythium irregulare CBS 494.86: understanding Eicosapentaenoic acid production. BMC Biotechnology, 19(41), 20191472-67501472-675010.1186/s12896-019-0529-331253157http://www.biomedcentral.com/bmcbiotechnolinfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-21T11:55:04Zoai:repositorium.sdum.uminho.pt:1822/60757Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T18:44:33.183926Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Genome-wide sequencing and metabolic annotation of Pythium irregulare CBS 494.86: understanding Eicosapentaenoic acid production
title Genome-wide sequencing and metabolic annotation of Pythium irregulare CBS 494.86: understanding Eicosapentaenoic acid production
spellingShingle Genome-wide sequencing and metabolic annotation of Pythium irregulare CBS 494.86: understanding Eicosapentaenoic acid production
Fernandes, B. S.
Eicosapentaenoic acid
Metabolic annotation
Pythium irregulare. unsaturated fatty acids, whole-genome sequence
Pythium irregulare
unsaturated fatty acids
whole-genome sequence
Science & Technology
title_short Genome-wide sequencing and metabolic annotation of Pythium irregulare CBS 494.86: understanding Eicosapentaenoic acid production
title_full Genome-wide sequencing and metabolic annotation of Pythium irregulare CBS 494.86: understanding Eicosapentaenoic acid production
title_fullStr Genome-wide sequencing and metabolic annotation of Pythium irregulare CBS 494.86: understanding Eicosapentaenoic acid production
title_full_unstemmed Genome-wide sequencing and metabolic annotation of Pythium irregulare CBS 494.86: understanding Eicosapentaenoic acid production
title_sort Genome-wide sequencing and metabolic annotation of Pythium irregulare CBS 494.86: understanding Eicosapentaenoic acid production
author Fernandes, B. S.
author_facet Fernandes, B. S.
Dias, Oscar
Costa, Gisela
Kaupert Neto, Antonio A.
Resende, Tiago F.
Oliveira, Juliana V. C.
Riaño-Pachón, Diego M.
Zaiat, Marcelo
Pradella, José G. C.
Rocha, I.
author_role author
author2 Dias, Oscar
Costa, Gisela
Kaupert Neto, Antonio A.
Resende, Tiago F.
Oliveira, Juliana V. C.
Riaño-Pachón, Diego M.
Zaiat, Marcelo
Pradella, José G. C.
Rocha, I.
author2_role author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade do Minho
dc.contributor.author.fl_str_mv Fernandes, B. S.
Dias, Oscar
Costa, Gisela
Kaupert Neto, Antonio A.
Resende, Tiago F.
Oliveira, Juliana V. C.
Riaño-Pachón, Diego M.
Zaiat, Marcelo
Pradella, José G. C.
Rocha, I.
dc.subject.por.fl_str_mv Eicosapentaenoic acid
Metabolic annotation
Pythium irregulare. unsaturated fatty acids, whole-genome sequence
Pythium irregulare
unsaturated fatty acids
whole-genome sequence
Science & Technology
topic Eicosapentaenoic acid
Metabolic annotation
Pythium irregulare. unsaturated fatty acids, whole-genome sequence
Pythium irregulare
unsaturated fatty acids
whole-genome sequence
Science & Technology
description Pythium irregulare is an oleaginous Oomycete able to accumulate large amounts of lipids, including Eicosapentaenoic acid (EPA). EPA is an important and expensive dietary supplement with a promising and very competitive market, which is dependent on fish-oil extraction. This has prompted several research groups to study biotechnological routes to obtain specific fatty acids rather than a mixture of various lipids. Moreover, microorganisms can use low cost carbon sources for lipid production, thus reducing production costs. Previous studies have highlighted the production of EPA by P. irregulare, exploiting diverse low cost carbon sources that are produced in large amounts, such as vinasse, glycerol, and food wastewater. However, there is still a lack of knowledge about its biosynthetic pathways, because no functional annotation of any Pythium sp. exists yet. The goal of this work was to identify key genes and pathways related to EPA biosynthesis, in P. irregulare CBS 494.86, by sequencing and performing an unprecedented annotation of its genome, considering the possibility of using wastewater as a carbon source.
publishDate 2019
dc.date.none.fl_str_mv 2019-12
2019-12-01T00:00:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/1822/60757
url http://hdl.handle.net/1822/60757
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Fernandes, B. S.; Dias, Oscar; Costa, Gisela; Kaupert Neto, Antonio A.; Resende, Tiago F.; Oliveira, Juliana V. C.; Riaño-Pachón, Diego M.; Zaiat, Marcelo; Pradella, José G. C.; Rocha, Isabel, Genome-wide sequencing and metabolic annotation of Pythium irregulare CBS 494.86: understanding Eicosapentaenoic acid production. BMC Biotechnology, 19(41), 2019
1472-6750
1472-6750
10.1186/s12896-019-0529-3
31253157
http://www.biomedcentral.com/bmcbiotechnol
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Springer Nature
publisher.none.fl_str_mv Springer Nature
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
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