Genome-wide sequencing and metabolic annotation of Pythium irregulare CBS 494.86: understanding Eicosapentaenoic acid production
Autor(a) principal: | |
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Data de Publicação: | 2019 |
Outros Autores: | , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/1822/60757 |
Resumo: | Pythium irregulare is an oleaginous Oomycete able to accumulate large amounts of lipids, including Eicosapentaenoic acid (EPA). EPA is an important and expensive dietary supplement with a promising and very competitive market, which is dependent on fish-oil extraction. This has prompted several research groups to study biotechnological routes to obtain specific fatty acids rather than a mixture of various lipids. Moreover, microorganisms can use low cost carbon sources for lipid production, thus reducing production costs. Previous studies have highlighted the production of EPA by P. irregulare, exploiting diverse low cost carbon sources that are produced in large amounts, such as vinasse, glycerol, and food wastewater. However, there is still a lack of knowledge about its biosynthetic pathways, because no functional annotation of any Pythium sp. exists yet. The goal of this work was to identify key genes and pathways related to EPA biosynthesis, in P. irregulare CBS 494.86, by sequencing and performing an unprecedented annotation of its genome, considering the possibility of using wastewater as a carbon source. |
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Genome-wide sequencing and metabolic annotation of Pythium irregulare CBS 494.86: understanding Eicosapentaenoic acid productionEicosapentaenoic acidMetabolic annotationPythium irregulare. unsaturated fatty acids, whole-genome sequencePythium irregulareunsaturated fatty acidswhole-genome sequenceScience & TechnologyPythium irregulare is an oleaginous Oomycete able to accumulate large amounts of lipids, including Eicosapentaenoic acid (EPA). EPA is an important and expensive dietary supplement with a promising and very competitive market, which is dependent on fish-oil extraction. This has prompted several research groups to study biotechnological routes to obtain specific fatty acids rather than a mixture of various lipids. Moreover, microorganisms can use low cost carbon sources for lipid production, thus reducing production costs. Previous studies have highlighted the production of EPA by P. irregulare, exploiting diverse low cost carbon sources that are produced in large amounts, such as vinasse, glycerol, and food wastewater. However, there is still a lack of knowledge about its biosynthetic pathways, because no functional annotation of any Pythium sp. exists yet. The goal of this work was to identify key genes and pathways related to EPA biosynthesis, in P. irregulare CBS 494.86, by sequencing and performing an unprecedented annotation of its genome, considering the possibility of using wastewater as a carbon source.The genome sequencing, strain acquisition and preliminary experiments and data analysis were funded by the São Paulo Research Foundation (FAPESP) and Coordination for the Improvement of Higher Education Personnel (CAPES) (Grante: 2016/10562–4). The experiment performance, data collection, analysis and interpretation of data were supported by the Portuguese Foundation for Science and Technology (FCT) under the scope of the strategic funding of [UID/BIO/04469] unit and COMPETE 2020 [POCI01-0145-FEDER-006684] and BioTecNorte operation [NORTE-01-0145-FEDER000004] funded by the European Regional Development Fund under the scope of Norte2020 - Programa Operacional Regional do Norte. The authors thank the project DD-DeCaF - Bioinformatics Services for Data-Driven Design of Cell Factories and Communities, Ref. H2020-LEIT-BIO-2015-1 686070–1, funded by the European Commission.info:eu-repo/semantics/publishedVersionSpringer NatureUniversidade do MinhoFernandes, B. S.Dias, OscarCosta, GiselaKaupert Neto, Antonio A.Resende, Tiago F.Oliveira, Juliana V. C.Riaño-Pachón, Diego M.Zaiat, MarceloPradella, José G. C.Rocha, I.2019-122019-12-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/1822/60757engFernandes, B. S.; Dias, Oscar; Costa, Gisela; Kaupert Neto, Antonio A.; Resende, Tiago F.; Oliveira, Juliana V. C.; Riaño-Pachón, Diego M.; Zaiat, Marcelo; Pradella, José G. C.; Rocha, Isabel, Genome-wide sequencing and metabolic annotation of Pythium irregulare CBS 494.86: understanding Eicosapentaenoic acid production. BMC Biotechnology, 19(41), 20191472-67501472-675010.1186/s12896-019-0529-331253157http://www.biomedcentral.com/bmcbiotechnolinfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-21T11:55:04Zoai:repositorium.sdum.uminho.pt:1822/60757Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T18:44:33.183926Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Genome-wide sequencing and metabolic annotation of Pythium irregulare CBS 494.86: understanding Eicosapentaenoic acid production |
title |
Genome-wide sequencing and metabolic annotation of Pythium irregulare CBS 494.86: understanding Eicosapentaenoic acid production |
spellingShingle |
Genome-wide sequencing and metabolic annotation of Pythium irregulare CBS 494.86: understanding Eicosapentaenoic acid production Fernandes, B. S. Eicosapentaenoic acid Metabolic annotation Pythium irregulare. unsaturated fatty acids, whole-genome sequence Pythium irregulare unsaturated fatty acids whole-genome sequence Science & Technology |
title_short |
Genome-wide sequencing and metabolic annotation of Pythium irregulare CBS 494.86: understanding Eicosapentaenoic acid production |
title_full |
Genome-wide sequencing and metabolic annotation of Pythium irregulare CBS 494.86: understanding Eicosapentaenoic acid production |
title_fullStr |
Genome-wide sequencing and metabolic annotation of Pythium irregulare CBS 494.86: understanding Eicosapentaenoic acid production |
title_full_unstemmed |
Genome-wide sequencing and metabolic annotation of Pythium irregulare CBS 494.86: understanding Eicosapentaenoic acid production |
title_sort |
Genome-wide sequencing and metabolic annotation of Pythium irregulare CBS 494.86: understanding Eicosapentaenoic acid production |
author |
Fernandes, B. S. |
author_facet |
Fernandes, B. S. Dias, Oscar Costa, Gisela Kaupert Neto, Antonio A. Resende, Tiago F. Oliveira, Juliana V. C. Riaño-Pachón, Diego M. Zaiat, Marcelo Pradella, José G. C. Rocha, I. |
author_role |
author |
author2 |
Dias, Oscar Costa, Gisela Kaupert Neto, Antonio A. Resende, Tiago F. Oliveira, Juliana V. C. Riaño-Pachón, Diego M. Zaiat, Marcelo Pradella, José G. C. Rocha, I. |
author2_role |
author author author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade do Minho |
dc.contributor.author.fl_str_mv |
Fernandes, B. S. Dias, Oscar Costa, Gisela Kaupert Neto, Antonio A. Resende, Tiago F. Oliveira, Juliana V. C. Riaño-Pachón, Diego M. Zaiat, Marcelo Pradella, José G. C. Rocha, I. |
dc.subject.por.fl_str_mv |
Eicosapentaenoic acid Metabolic annotation Pythium irregulare. unsaturated fatty acids, whole-genome sequence Pythium irregulare unsaturated fatty acids whole-genome sequence Science & Technology |
topic |
Eicosapentaenoic acid Metabolic annotation Pythium irregulare. unsaturated fatty acids, whole-genome sequence Pythium irregulare unsaturated fatty acids whole-genome sequence Science & Technology |
description |
Pythium irregulare is an oleaginous Oomycete able to accumulate large amounts of lipids, including Eicosapentaenoic acid (EPA). EPA is an important and expensive dietary supplement with a promising and very competitive market, which is dependent on fish-oil extraction. This has prompted several research groups to study biotechnological routes to obtain specific fatty acids rather than a mixture of various lipids. Moreover, microorganisms can use low cost carbon sources for lipid production, thus reducing production costs. Previous studies have highlighted the production of EPA by P. irregulare, exploiting diverse low cost carbon sources that are produced in large amounts, such as vinasse, glycerol, and food wastewater. However, there is still a lack of knowledge about its biosynthetic pathways, because no functional annotation of any Pythium sp. exists yet. The goal of this work was to identify key genes and pathways related to EPA biosynthesis, in P. irregulare CBS 494.86, by sequencing and performing an unprecedented annotation of its genome, considering the possibility of using wastewater as a carbon source. |
publishDate |
2019 |
dc.date.none.fl_str_mv |
2019-12 2019-12-01T00:00:00Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/1822/60757 |
url |
http://hdl.handle.net/1822/60757 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Fernandes, B. S.; Dias, Oscar; Costa, Gisela; Kaupert Neto, Antonio A.; Resende, Tiago F.; Oliveira, Juliana V. C.; Riaño-Pachón, Diego M.; Zaiat, Marcelo; Pradella, José G. C.; Rocha, Isabel, Genome-wide sequencing and metabolic annotation of Pythium irregulare CBS 494.86: understanding Eicosapentaenoic acid production. BMC Biotechnology, 19(41), 2019 1472-6750 1472-6750 10.1186/s12896-019-0529-3 31253157 http://www.biomedcentral.com/bmcbiotechnol |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Springer Nature |
publisher.none.fl_str_mv |
Springer Nature |
dc.source.none.fl_str_mv |
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