Linking cholinesterase inhibition with behavioural changes in the sea snail Gibbula umbilicalis: Effects of the organophosphate pesticide chlorpyrifos
Autor(a) principal: | |
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Data de Publicação: | 2019 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10400.8/4212 |
Resumo: | This study was funded by Fundação para a Ciência e a Tecnologia (FCT) through the Strategic Project UID/MAR/04292/2013, project MARINE INVADERS – The impact and mechanisms of success of the invasive seaweed Asparagopsis armata on coastal environments (POCI01-0145-FEDER-031144), and the grants awarded to Carla Silva (SFRH/BD/87030/2012), Luís Alves (SFRH/BD/122082/2016) and Sara Novais (SFRH/BPD/94500/2013). The project was also partially funded by the Integrated Programme of SR&TD “SmartBioR” (reference |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Linking cholinesterase inhibition with behavioural changes in the sea snail Gibbula umbilicalis: Effects of the organophosphate pesticide chlorpyrifosAcetylcholinesterase activityBehaviourCholinesterases characterizationEcotoxicologyMarine snailsThis study was funded by Fundação para a Ciência e a Tecnologia (FCT) through the Strategic Project UID/MAR/04292/2013, project MARINE INVADERS – The impact and mechanisms of success of the invasive seaweed Asparagopsis armata on coastal environments (POCI01-0145-FEDER-031144), and the grants awarded to Carla Silva (SFRH/BD/87030/2012), Luís Alves (SFRH/BD/122082/2016) and Sara Novais (SFRH/BPD/94500/2013). The project was also partially funded by the Integrated Programme of SR&TD “SmartBioR” (referenceInhibition of acetylcholinesterase (AChE) activity has been widely used to assess the exposure and effects of anticholinergic environmental contaminants in several species. The aim of this study was to investigate if sublethal concentrations of the organophosphorous pesticide chlorpyrifos (CPF), a well-known AChE inhibitor, would also affect cholinesterases (ChE) in Gibbula umbilicalis and if this inhibition would result in an alteration of its behaviour, in an attempt to link the effects observed at the cellular level with effects at higher levels of ecological relevance. The biochemical properties of ChEs in this species were first characterized through the assessment of different enzymatic forms present in the sea snail, using different substrates and selective inhibitors. The results suggest that G. umbilicalis possess ChEs with characteristics of typical AChE, which should be the main form present. Additionally, in vitro and in vivo effects of CPF on AChE activity were investigated, along with effects on snails' behaviour: the ability of the snails to move/turn after exposure to the contaminant (flipping test). As expected, CPF inhibited AChE activity both in vitro and in vivo conditions. Moreover, the link between AChE activity inhibition and adverse effects on behavioural changes was established: AChE inhibition was positively correlated with the flipping test, indicating a mechanistic relationship between the two endpoints determined in in vivo exposures. This study highlights the importance of linking biochemical endpoints such as AChE activity with higher level endpoints like behavioural alterations, increasing the ecological relevance of the effects observed.IC-OnlineSilva, Carla O.Novais, Sara C.Alves, Luís M. F.Soares, Amadeu M. V. M.Barata, CarlosLemos, Marco F. L.2019-10-16T10:36:47Z2019-112019-10-07T10:37:11Z2019-11-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10400.8/4212engSilva, C. O., Novais, S. C., Alves, L. M. F., Lemos, M. F. L., Soares, A. M. V. M., & Barata, C. (2019). Linking cholinesterase inhibition with behavioural changes in the sea snail Gibbula umbilicalis: Effects of the organophosphate pesticide chlorpyrifos. Comparative Biochemistry and Physiology Part - C: Toxicology and Pharmacology, 225. https://doi.org/10.1016/j.cbpc.2019.1085701532-0456cv-prod-69890510.1016/j.cbpc.2019.108570metadata only accessinfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-01-17T15:48:48Zoai:iconline.ipleiria.pt:10400.8/4212Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T01:48:07.277334Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Linking cholinesterase inhibition with behavioural changes in the sea snail Gibbula umbilicalis: Effects of the organophosphate pesticide chlorpyrifos |
title |
Linking cholinesterase inhibition with behavioural changes in the sea snail Gibbula umbilicalis: Effects of the organophosphate pesticide chlorpyrifos |
spellingShingle |
Linking cholinesterase inhibition with behavioural changes in the sea snail Gibbula umbilicalis: Effects of the organophosphate pesticide chlorpyrifos Silva, Carla O. Acetylcholinesterase activity Behaviour Cholinesterases characterization Ecotoxicology Marine snails |
title_short |
Linking cholinesterase inhibition with behavioural changes in the sea snail Gibbula umbilicalis: Effects of the organophosphate pesticide chlorpyrifos |
title_full |
Linking cholinesterase inhibition with behavioural changes in the sea snail Gibbula umbilicalis: Effects of the organophosphate pesticide chlorpyrifos |
title_fullStr |
Linking cholinesterase inhibition with behavioural changes in the sea snail Gibbula umbilicalis: Effects of the organophosphate pesticide chlorpyrifos |
title_full_unstemmed |
Linking cholinesterase inhibition with behavioural changes in the sea snail Gibbula umbilicalis: Effects of the organophosphate pesticide chlorpyrifos |
title_sort |
Linking cholinesterase inhibition with behavioural changes in the sea snail Gibbula umbilicalis: Effects of the organophosphate pesticide chlorpyrifos |
author |
Silva, Carla O. |
author_facet |
Silva, Carla O. Novais, Sara C. Alves, Luís M. F. Soares, Amadeu M. V. M. Barata, Carlos Lemos, Marco F. L. |
author_role |
author |
author2 |
Novais, Sara C. Alves, Luís M. F. Soares, Amadeu M. V. M. Barata, Carlos Lemos, Marco F. L. |
author2_role |
author author author author author |
dc.contributor.none.fl_str_mv |
IC-Online |
dc.contributor.author.fl_str_mv |
Silva, Carla O. Novais, Sara C. Alves, Luís M. F. Soares, Amadeu M. V. M. Barata, Carlos Lemos, Marco F. L. |
dc.subject.por.fl_str_mv |
Acetylcholinesterase activity Behaviour Cholinesterases characterization Ecotoxicology Marine snails |
topic |
Acetylcholinesterase activity Behaviour Cholinesterases characterization Ecotoxicology Marine snails |
description |
This study was funded by Fundação para a Ciência e a Tecnologia (FCT) through the Strategic Project UID/MAR/04292/2013, project MARINE INVADERS – The impact and mechanisms of success of the invasive seaweed Asparagopsis armata on coastal environments (POCI01-0145-FEDER-031144), and the grants awarded to Carla Silva (SFRH/BD/87030/2012), Luís Alves (SFRH/BD/122082/2016) and Sara Novais (SFRH/BPD/94500/2013). The project was also partially funded by the Integrated Programme of SR&TD “SmartBioR” (reference |
publishDate |
2019 |
dc.date.none.fl_str_mv |
2019-10-16T10:36:47Z 2019-11 2019-10-07T10:37:11Z 2019-11-01T00:00:00Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10400.8/4212 |
url |
http://hdl.handle.net/10400.8/4212 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Silva, C. O., Novais, S. C., Alves, L. M. F., Lemos, M. F. L., Soares, A. M. V. M., & Barata, C. (2019). Linking cholinesterase inhibition with behavioural changes in the sea snail Gibbula umbilicalis: Effects of the organophosphate pesticide chlorpyrifos. Comparative Biochemistry and Physiology Part - C: Toxicology and Pharmacology, 225. https://doi.org/10.1016/j.cbpc.2019.108570 1532-0456 cv-prod-698905 10.1016/j.cbpc.2019.108570 |
dc.rights.driver.fl_str_mv |
metadata only access info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
metadata only access |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
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Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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RCAAP |
institution |
RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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1799136974303395840 |