Linking cholinesterase inhibition with behavioural changes in the sea snail Gibbula umbilicalis: Effects of the organophosphate pesticide chlorpyrifos

Detalhes bibliográficos
Autor(a) principal: Silva, Carla O.
Data de Publicação: 2019
Outros Autores: Novais, Sara C., Alves, Luís M. F., Soares, Amadeu M. V. M., Barata, Carlos, Lemos, Marco F. L.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10400.8/4212
Resumo: This study was funded by Fundação para a Ciência e a Tecnologia (FCT) through the Strategic Project UID/MAR/04292/2013, project MARINE INVADERS – The impact and mechanisms of success of the invasive seaweed Asparagopsis armata on coastal environments (POCI01-0145-FEDER-031144), and the grants awarded to Carla Silva (SFRH/BD/87030/2012), Luís Alves (SFRH/BD/122082/2016) and Sara Novais (SFRH/BPD/94500/2013). The project was also partially funded by the Integrated Programme of SR&TD “SmartBioR” (reference
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spelling Linking cholinesterase inhibition with behavioural changes in the sea snail Gibbula umbilicalis: Effects of the organophosphate pesticide chlorpyrifosAcetylcholinesterase activityBehaviourCholinesterases characterizationEcotoxicologyMarine snailsThis study was funded by Fundação para a Ciência e a Tecnologia (FCT) through the Strategic Project UID/MAR/04292/2013, project MARINE INVADERS – The impact and mechanisms of success of the invasive seaweed Asparagopsis armata on coastal environments (POCI01-0145-FEDER-031144), and the grants awarded to Carla Silva (SFRH/BD/87030/2012), Luís Alves (SFRH/BD/122082/2016) and Sara Novais (SFRH/BPD/94500/2013). The project was also partially funded by the Integrated Programme of SR&TD “SmartBioR” (referenceInhibition of acetylcholinesterase (AChE) activity has been widely used to assess the exposure and effects of anticholinergic environmental contaminants in several species. The aim of this study was to investigate if sublethal concentrations of the organophosphorous pesticide chlorpyrifos (CPF), a well-known AChE inhibitor, would also affect cholinesterases (ChE) in Gibbula umbilicalis and if this inhibition would result in an alteration of its behaviour, in an attempt to link the effects observed at the cellular level with effects at higher levels of ecological relevance. The biochemical properties of ChEs in this species were first characterized through the assessment of different enzymatic forms present in the sea snail, using different substrates and selective inhibitors. The results suggest that G. umbilicalis possess ChEs with characteristics of typical AChE, which should be the main form present. Additionally, in vitro and in vivo effects of CPF on AChE activity were investigated, along with effects on snails' behaviour: the ability of the snails to move/turn after exposure to the contaminant (flipping test). As expected, CPF inhibited AChE activity both in vitro and in vivo conditions. Moreover, the link between AChE activity inhibition and adverse effects on behavioural changes was established: AChE inhibition was positively correlated with the flipping test, indicating a mechanistic relationship between the two endpoints determined in in vivo exposures. This study highlights the importance of linking biochemical endpoints such as AChE activity with higher level endpoints like behavioural alterations, increasing the ecological relevance of the effects observed.IC-OnlineSilva, Carla O.Novais, Sara C.Alves, Luís M. F.Soares, Amadeu M. V. M.Barata, CarlosLemos, Marco F. L.2019-10-16T10:36:47Z2019-112019-10-07T10:37:11Z2019-11-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10400.8/4212engSilva, C. O., Novais, S. C., Alves, L. M. F., Lemos, M. F. L., Soares, A. M. V. M., & Barata, C. (2019). Linking cholinesterase inhibition with behavioural changes in the sea snail Gibbula umbilicalis: Effects of the organophosphate pesticide chlorpyrifos. Comparative Biochemistry and Physiology Part - C: Toxicology and Pharmacology, 225. https://doi.org/10.1016/j.cbpc.2019.1085701532-0456cv-prod-69890510.1016/j.cbpc.2019.108570metadata only accessinfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-01-17T15:48:48Zoai:iconline.ipleiria.pt:10400.8/4212Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T01:48:07.277334Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Linking cholinesterase inhibition with behavioural changes in the sea snail Gibbula umbilicalis: Effects of the organophosphate pesticide chlorpyrifos
title Linking cholinesterase inhibition with behavioural changes in the sea snail Gibbula umbilicalis: Effects of the organophosphate pesticide chlorpyrifos
spellingShingle Linking cholinesterase inhibition with behavioural changes in the sea snail Gibbula umbilicalis: Effects of the organophosphate pesticide chlorpyrifos
Silva, Carla O.
Acetylcholinesterase activity
Behaviour
Cholinesterases characterization
Ecotoxicology
Marine snails
title_short Linking cholinesterase inhibition with behavioural changes in the sea snail Gibbula umbilicalis: Effects of the organophosphate pesticide chlorpyrifos
title_full Linking cholinesterase inhibition with behavioural changes in the sea snail Gibbula umbilicalis: Effects of the organophosphate pesticide chlorpyrifos
title_fullStr Linking cholinesterase inhibition with behavioural changes in the sea snail Gibbula umbilicalis: Effects of the organophosphate pesticide chlorpyrifos
title_full_unstemmed Linking cholinesterase inhibition with behavioural changes in the sea snail Gibbula umbilicalis: Effects of the organophosphate pesticide chlorpyrifos
title_sort Linking cholinesterase inhibition with behavioural changes in the sea snail Gibbula umbilicalis: Effects of the organophosphate pesticide chlorpyrifos
author Silva, Carla O.
author_facet Silva, Carla O.
Novais, Sara C.
Alves, Luís M. F.
Soares, Amadeu M. V. M.
Barata, Carlos
Lemos, Marco F. L.
author_role author
author2 Novais, Sara C.
Alves, Luís M. F.
Soares, Amadeu M. V. M.
Barata, Carlos
Lemos, Marco F. L.
author2_role author
author
author
author
author
dc.contributor.none.fl_str_mv IC-Online
dc.contributor.author.fl_str_mv Silva, Carla O.
Novais, Sara C.
Alves, Luís M. F.
Soares, Amadeu M. V. M.
Barata, Carlos
Lemos, Marco F. L.
dc.subject.por.fl_str_mv Acetylcholinesterase activity
Behaviour
Cholinesterases characterization
Ecotoxicology
Marine snails
topic Acetylcholinesterase activity
Behaviour
Cholinesterases characterization
Ecotoxicology
Marine snails
description This study was funded by Fundação para a Ciência e a Tecnologia (FCT) through the Strategic Project UID/MAR/04292/2013, project MARINE INVADERS – The impact and mechanisms of success of the invasive seaweed Asparagopsis armata on coastal environments (POCI01-0145-FEDER-031144), and the grants awarded to Carla Silva (SFRH/BD/87030/2012), Luís Alves (SFRH/BD/122082/2016) and Sara Novais (SFRH/BPD/94500/2013). The project was also partially funded by the Integrated Programme of SR&TD “SmartBioR” (reference
publishDate 2019
dc.date.none.fl_str_mv 2019-10-16T10:36:47Z
2019-11
2019-10-07T10:37:11Z
2019-11-01T00:00:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10400.8/4212
url http://hdl.handle.net/10400.8/4212
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Silva, C. O., Novais, S. C., Alves, L. M. F., Lemos, M. F. L., Soares, A. M. V. M., & Barata, C. (2019). Linking cholinesterase inhibition with behavioural changes in the sea snail Gibbula umbilicalis: Effects of the organophosphate pesticide chlorpyrifos. Comparative Biochemistry and Physiology Part - C: Toxicology and Pharmacology, 225. https://doi.org/10.1016/j.cbpc.2019.108570
1532-0456
cv-prod-698905
10.1016/j.cbpc.2019.108570
dc.rights.driver.fl_str_mv metadata only access
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dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
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repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
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