Cytotoxic and hemolytic activities of extracts of the fish parasite dinoflagellate amyloodinium ocellatum
Autor(a) principal: | |
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Data de Publicação: | 2022 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10400.1/18097 |
Resumo: | The dinoflagellate Amyloodinium ocellatum is the etiological agent of a parasitic disease named amyloodiniosis. Mortalities of diseased fish are usually attributed to anoxia, osmoregulatory impairment, or opportunistic bacterial infections. Nevertheless, the phylogenetic proximity of A. ocellatum to a group of toxin-producing dinoflagellates from Pfiesteria, Parvodinium and Paulsenella genera suggests that it may produce toxin-like compounds, adding a new dimension to the possible cause of mortalities in A. ocellatum outbreaks. To address this question, extracts prepared from different life stages of the parasite were tested in vitro for cytotoxic effects using two cell lines derived from branchial arches (ABSa15) and the caudal fin (CFSa1) of the gilthead seabream (Sparus aurata), and for hemolytic effects using erythrocytes purified from the blood of gilthead seabream juveniles. Cytotoxicity and a strong hemolytic effect, similar to those observed for Karlodinium toxins, were observed for the less polar extracts of the parasitic stage (trophont). A similar trend was observed for the less polar extracts of the infective stage (dinospores), although cell viability was only affected in the ABSa15 line. These results suggest that A. ocellatum produces tissue-specific toxic compounds that may have a role in the attachment of the dinospores’ and trophonts’ feeding process. |
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Cytotoxic and hemolytic activities of extracts of the fish parasite dinoflagellate amyloodinium ocellatumFish pathologyEctoparasiteToxicological responseHemolysisCell viabilityThe dinoflagellate Amyloodinium ocellatum is the etiological agent of a parasitic disease named amyloodiniosis. Mortalities of diseased fish are usually attributed to anoxia, osmoregulatory impairment, or opportunistic bacterial infections. Nevertheless, the phylogenetic proximity of A. ocellatum to a group of toxin-producing dinoflagellates from Pfiesteria, Parvodinium and Paulsenella genera suggests that it may produce toxin-like compounds, adding a new dimension to the possible cause of mortalities in A. ocellatum outbreaks. To address this question, extracts prepared from different life stages of the parasite were tested in vitro for cytotoxic effects using two cell lines derived from branchial arches (ABSa15) and the caudal fin (CFSa1) of the gilthead seabream (Sparus aurata), and for hemolytic effects using erythrocytes purified from the blood of gilthead seabream juveniles. Cytotoxicity and a strong hemolytic effect, similar to those observed for Karlodinium toxins, were observed for the less polar extracts of the parasitic stage (trophont). A similar trend was observed for the less polar extracts of the infective stage (dinospores), although cell viability was only affected in the ABSa15 line. These results suggest that A. ocellatum produces tissue-specific toxic compounds that may have a role in the attachment of the dinospores’ and trophonts’ feeding process.MDPISapientiaMoreira, MárcioSoliño, LuciaMarques, Cátia L.Laizé, VincentPousão-Ferreira, PedroFidalgo E Costa, PedroSoares, Florbela2022-07-26T12:52:45Z2022-07-082022-07-25T16:32:45Z2022-07-08T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10400.1/18097engToxins 14 (7): 467 (2022)10.3390/toxins140704672072-6651info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-24T10:30:20Zoai:sapientia.ualg.pt:10400.1/18097Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T20:07:54.038824Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Cytotoxic and hemolytic activities of extracts of the fish parasite dinoflagellate amyloodinium ocellatum |
title |
Cytotoxic and hemolytic activities of extracts of the fish parasite dinoflagellate amyloodinium ocellatum |
spellingShingle |
Cytotoxic and hemolytic activities of extracts of the fish parasite dinoflagellate amyloodinium ocellatum Moreira, Márcio Fish pathology Ectoparasite Toxicological response Hemolysis Cell viability |
title_short |
Cytotoxic and hemolytic activities of extracts of the fish parasite dinoflagellate amyloodinium ocellatum |
title_full |
Cytotoxic and hemolytic activities of extracts of the fish parasite dinoflagellate amyloodinium ocellatum |
title_fullStr |
Cytotoxic and hemolytic activities of extracts of the fish parasite dinoflagellate amyloodinium ocellatum |
title_full_unstemmed |
Cytotoxic and hemolytic activities of extracts of the fish parasite dinoflagellate amyloodinium ocellatum |
title_sort |
Cytotoxic and hemolytic activities of extracts of the fish parasite dinoflagellate amyloodinium ocellatum |
author |
Moreira, Márcio |
author_facet |
Moreira, Márcio Soliño, Lucia Marques, Cátia L. Laizé, Vincent Pousão-Ferreira, Pedro Fidalgo E Costa, Pedro Soares, Florbela |
author_role |
author |
author2 |
Soliño, Lucia Marques, Cátia L. Laizé, Vincent Pousão-Ferreira, Pedro Fidalgo E Costa, Pedro Soares, Florbela |
author2_role |
author author author author author author |
dc.contributor.none.fl_str_mv |
Sapientia |
dc.contributor.author.fl_str_mv |
Moreira, Márcio Soliño, Lucia Marques, Cátia L. Laizé, Vincent Pousão-Ferreira, Pedro Fidalgo E Costa, Pedro Soares, Florbela |
dc.subject.por.fl_str_mv |
Fish pathology Ectoparasite Toxicological response Hemolysis Cell viability |
topic |
Fish pathology Ectoparasite Toxicological response Hemolysis Cell viability |
description |
The dinoflagellate Amyloodinium ocellatum is the etiological agent of a parasitic disease named amyloodiniosis. Mortalities of diseased fish are usually attributed to anoxia, osmoregulatory impairment, or opportunistic bacterial infections. Nevertheless, the phylogenetic proximity of A. ocellatum to a group of toxin-producing dinoflagellates from Pfiesteria, Parvodinium and Paulsenella genera suggests that it may produce toxin-like compounds, adding a new dimension to the possible cause of mortalities in A. ocellatum outbreaks. To address this question, extracts prepared from different life stages of the parasite were tested in vitro for cytotoxic effects using two cell lines derived from branchial arches (ABSa15) and the caudal fin (CFSa1) of the gilthead seabream (Sparus aurata), and for hemolytic effects using erythrocytes purified from the blood of gilthead seabream juveniles. Cytotoxicity and a strong hemolytic effect, similar to those observed for Karlodinium toxins, were observed for the less polar extracts of the parasitic stage (trophont). A similar trend was observed for the less polar extracts of the infective stage (dinospores), although cell viability was only affected in the ABSa15 line. These results suggest that A. ocellatum produces tissue-specific toxic compounds that may have a role in the attachment of the dinospores’ and trophonts’ feeding process. |
publishDate |
2022 |
dc.date.none.fl_str_mv |
2022-07-26T12:52:45Z 2022-07-08 2022-07-25T16:32:45Z 2022-07-08T00:00:00Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10400.1/18097 |
url |
http://hdl.handle.net/10400.1/18097 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Toxins 14 (7): 467 (2022) 10.3390/toxins14070467 2072-6651 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
MDPI |
publisher.none.fl_str_mv |
MDPI |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
instname_str |
Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
instacron_str |
RCAAP |
institution |
RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
collection |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository.name.fl_str_mv |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
repository.mail.fl_str_mv |
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1799133324434735104 |