Flexible sensing devices integrating molecularly-imprinted polymers for the detection of 3-nitrotyrosine biomarker
Autor(a) principal: | |
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Data de Publicação: | 2022 |
Outros Autores: | , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10316/103198 https://doi.org/10.1016/j.biosx.2022.100107 |
Resumo: | In recent years, the development of flexible and wearable devices for healthcare and biomedical applications has become an emerging technological goal, particularly with personalized medicine on the rise. As a response to the increasing demand for in-situ sensing platforms that fulfil some essential requirements like sensitivity, reproducibility and high stability, electrochemical sensors have boosted their way for innovative approaches. So, highquality flexible sensing strategies are still a demand for local monitoring. Herein, a flexible three-electrode system was fabricated on transparent polymeric sheet substrate through physical deposition of gold as working, counter, and reference electrodes. Along the fabrication process, the electrochemical performance of these electrodes was assessed by means of cyclic voltammetry (CV) while gold adherence to the plastic material was continuously improved. Afterwards, a high-performance molecularlyimprinted sensing film inspired by natural recognition mechanism was assembled through electropolymerization of phenol monomer, in the presence of 3-nitrotyrosine (3-NT), directly on the gold surface. Under the optimized conditions, the flexible (bio)sensor platform was able to detect the presence of 3-NT over the concentration range 10 pg/mL – 1 μg/mL, enabling one of the lower limits of detection found in the literature (1.13 pg/mL or 24.9 pM). The obtained (bio)sensor displayed good reproducibility, stability and selectivity over the chosen interfering substances. Overall, the developed electrochemical device may serve as a flexible, miniaturized, and reliable platform, with potential to be applied in the future as wearable sensing technology. |
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Flexible sensing devices integrating molecularly-imprinted polymers for the detection of 3-nitrotyrosine biomarkerMolecularly-imprinted polymerFlexible substrateGold-based electrodesElectrochemical (bio)sensorOxidative stress biomarkerIn recent years, the development of flexible and wearable devices for healthcare and biomedical applications has become an emerging technological goal, particularly with personalized medicine on the rise. As a response to the increasing demand for in-situ sensing platforms that fulfil some essential requirements like sensitivity, reproducibility and high stability, electrochemical sensors have boosted their way for innovative approaches. So, highquality flexible sensing strategies are still a demand for local monitoring. Herein, a flexible three-electrode system was fabricated on transparent polymeric sheet substrate through physical deposition of gold as working, counter, and reference electrodes. Along the fabrication process, the electrochemical performance of these electrodes was assessed by means of cyclic voltammetry (CV) while gold adherence to the plastic material was continuously improved. Afterwards, a high-performance molecularlyimprinted sensing film inspired by natural recognition mechanism was assembled through electropolymerization of phenol monomer, in the presence of 3-nitrotyrosine (3-NT), directly on the gold surface. Under the optimized conditions, the flexible (bio)sensor platform was able to detect the presence of 3-NT over the concentration range 10 pg/mL – 1 μg/mL, enabling one of the lower limits of detection found in the literature (1.13 pg/mL or 24.9 pM). The obtained (bio)sensor displayed good reproducibility, stability and selectivity over the chosen interfering substances. Overall, the developed electrochemical device may serve as a flexible, miniaturized, and reliable platform, with potential to be applied in the future as wearable sensing technology.2022info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://hdl.handle.net/10316/103198http://hdl.handle.net/10316/103198https://doi.org/10.1016/j.biosx.2022.100107eng25901370Martins, G. V.Riveiro, A.Chiussi, S.Sales, M. G. F.info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2022-10-21T20:31:46Zoai:estudogeral.uc.pt:10316/103198Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T21:20:04.424281Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Flexible sensing devices integrating molecularly-imprinted polymers for the detection of 3-nitrotyrosine biomarker |
title |
Flexible sensing devices integrating molecularly-imprinted polymers for the detection of 3-nitrotyrosine biomarker |
spellingShingle |
Flexible sensing devices integrating molecularly-imprinted polymers for the detection of 3-nitrotyrosine biomarker Martins, G. V. Molecularly-imprinted polymer Flexible substrate Gold-based electrodes Electrochemical (bio)sensor Oxidative stress biomarker |
title_short |
Flexible sensing devices integrating molecularly-imprinted polymers for the detection of 3-nitrotyrosine biomarker |
title_full |
Flexible sensing devices integrating molecularly-imprinted polymers for the detection of 3-nitrotyrosine biomarker |
title_fullStr |
Flexible sensing devices integrating molecularly-imprinted polymers for the detection of 3-nitrotyrosine biomarker |
title_full_unstemmed |
Flexible sensing devices integrating molecularly-imprinted polymers for the detection of 3-nitrotyrosine biomarker |
title_sort |
Flexible sensing devices integrating molecularly-imprinted polymers for the detection of 3-nitrotyrosine biomarker |
author |
Martins, G. V. |
author_facet |
Martins, G. V. Riveiro, A. Chiussi, S. Sales, M. G. F. |
author_role |
author |
author2 |
Riveiro, A. Chiussi, S. Sales, M. G. F. |
author2_role |
author author author |
dc.contributor.author.fl_str_mv |
Martins, G. V. Riveiro, A. Chiussi, S. Sales, M. G. F. |
dc.subject.por.fl_str_mv |
Molecularly-imprinted polymer Flexible substrate Gold-based electrodes Electrochemical (bio)sensor Oxidative stress biomarker |
topic |
Molecularly-imprinted polymer Flexible substrate Gold-based electrodes Electrochemical (bio)sensor Oxidative stress biomarker |
description |
In recent years, the development of flexible and wearable devices for healthcare and biomedical applications has become an emerging technological goal, particularly with personalized medicine on the rise. As a response to the increasing demand for in-situ sensing platforms that fulfil some essential requirements like sensitivity, reproducibility and high stability, electrochemical sensors have boosted their way for innovative approaches. So, highquality flexible sensing strategies are still a demand for local monitoring. Herein, a flexible three-electrode system was fabricated on transparent polymeric sheet substrate through physical deposition of gold as working, counter, and reference electrodes. Along the fabrication process, the electrochemical performance of these electrodes was assessed by means of cyclic voltammetry (CV) while gold adherence to the plastic material was continuously improved. Afterwards, a high-performance molecularlyimprinted sensing film inspired by natural recognition mechanism was assembled through electropolymerization of phenol monomer, in the presence of 3-nitrotyrosine (3-NT), directly on the gold surface. Under the optimized conditions, the flexible (bio)sensor platform was able to detect the presence of 3-NT over the concentration range 10 pg/mL – 1 μg/mL, enabling one of the lower limits of detection found in the literature (1.13 pg/mL or 24.9 pM). The obtained (bio)sensor displayed good reproducibility, stability and selectivity over the chosen interfering substances. Overall, the developed electrochemical device may serve as a flexible, miniaturized, and reliable platform, with potential to be applied in the future as wearable sensing technology. |
publishDate |
2022 |
dc.date.none.fl_str_mv |
2022 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10316/103198 http://hdl.handle.net/10316/103198 https://doi.org/10.1016/j.biosx.2022.100107 |
url |
http://hdl.handle.net/10316/103198 https://doi.org/10.1016/j.biosx.2022.100107 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
25901370 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
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Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
instacron_str |
RCAAP |
institution |
RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
collection |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository.name.fl_str_mv |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
repository.mail.fl_str_mv |
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1799134094455472128 |