Study of the immunologic response of marine-derived collagen and gelatin extracts for tissue engineering applications
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2022 |
| Outros Autores: | , , , , , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
| Texto Completo: | https://hdl.handle.net/1822/76392 |
Resumo: | Supplementary material associated with this article can be found, in the online version, at doi: 10.1016/j.actbio.2022.01.009 . |
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Study of the immunologic response of marine-derived collagen and gelatin extracts for tissue engineering applicationsAnti-inflammatory macrophagesimmunogenicityM2-like macrophagesmarine biomaterialstype I collagenScience & TechnologySupplementary material associated with this article can be found, in the online version, at doi: 10.1016/j.actbio.2022.01.009 .The host immunologic response to a specific material is a critical aspect when considering it for clinical implementation. Collagen and gelatin extracted from marine sources have been proposed as biomaterials for tissue engineering applications, but there is a lack of information in the literature about their immunogenicity. In this work, we evaluated the immune response to collagen and/or gelatin from blue shark and codfish, previously extracted and characterized. After endotoxin evaluation, bone marrow-derived macrophages were exposed to the materials and a panel of pro- and anti-inflammatory cytokines were evaluated both for protein quantification and gene expression. Then, the impact of those materials in the host was evaluated through peritoneal injection in C57BL/6 mice. The results suggested shark collagen as the less immunogenic material, inducing low expression of pro-inflammatory cytokines as well as inducible nitric oxide synthase (encoded by Nos2) and high expression of Arginase 1 (encoded by Arg1). Although shark gelatin appeared to be the material with higher pro-inflammatory expression, it also presents a high expression of IL-10 (anti-inflammatory cytokine) and Arginase (both markers for M2-like macrophages). When injected in the peritoneal cavity of mice, our materials demonstrated a transient recruitment of neutrophil, being almost non-existent after 24 hours of injection. Based on these findings, the studied collagenous materials can be considered interesting biomaterial candidates for regenerative medicine as they may induce an activation of the M2-like macrophage population, which is involved in suppressing the inflammatory processes promoting tissue remodeling.The authors would like to acknowledge to European Union for the financial support under the scope of European Regional Development Fund (ERDF) through the projects 0302_CVMAR_I_1_P and 0474_BLUEBIOLAB_1_E (program INTERREG España-Portugal 2014/2020). Funding from the Portuguese Foundation for Science and Technology for the grant of A. L. Alves (Ana Luísa Alves) under Doctoral Programme Do ∗Mar (PD/BD/127995/2016) is also ac- knowledged.Elsevier 1Universidade do MinhoAlves, A. L.Costa-Gouveia, J.Vieira de Castro, J.Sotelo, C. G.Vázquez, J. A.Pérez Martín, R. I.Torrado, E.Neves, N. M.Reis, R. L.Castro, A. G.Silva, Tiago H.2022-012022-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttps://hdl.handle.net/1822/76392engAlves A. L., Costa-Gouveia J., Vieira de Castro J., Sotelo C. G., Vázquez J. A., Pérez Martín R. I., Torrado E., Neves N. M., Reis R. L., Castro A. G., Silva T. H. Study of the immunologic response of marine-derived collagen and gelatin extracts for tissue engineering applications, Acta Biomaterialia, Vol. 141, pp. 123-131, doi:10.1016/j.actbio.2022.01.009, 20221742-706110.1016/j.actbio.2022.01.00935017072https://doi.org/10.1016/j.actbio.2022.01.009info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-07-13T02:09:01Zoai:repositorium.sdum.uminho.pt:1822/76392Portal AgregadorONGhttps://www.rcaap.pt/oai/openairemluisa.alvim@gmail.comopendoar:71602024-07-13T02:09:01Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
| dc.title.none.fl_str_mv |
Study of the immunologic response of marine-derived collagen and gelatin extracts for tissue engineering applications |
| title |
Study of the immunologic response of marine-derived collagen and gelatin extracts for tissue engineering applications |
| spellingShingle |
Study of the immunologic response of marine-derived collagen and gelatin extracts for tissue engineering applications Alves, A. L. Anti-inflammatory macrophages immunogenicity M2-like macrophages marine biomaterials type I collagen Science & Technology |
| title_short |
Study of the immunologic response of marine-derived collagen and gelatin extracts for tissue engineering applications |
| title_full |
Study of the immunologic response of marine-derived collagen and gelatin extracts for tissue engineering applications |
| title_fullStr |
Study of the immunologic response of marine-derived collagen and gelatin extracts for tissue engineering applications |
| title_full_unstemmed |
Study of the immunologic response of marine-derived collagen and gelatin extracts for tissue engineering applications |
| title_sort |
Study of the immunologic response of marine-derived collagen and gelatin extracts for tissue engineering applications |
| author |
Alves, A. L. |
| author_facet |
Alves, A. L. Costa-Gouveia, J. Vieira de Castro, J. Sotelo, C. G. Vázquez, J. A. Pérez Martín, R. I. Torrado, E. Neves, N. M. Reis, R. L. Castro, A. G. Silva, Tiago H. |
| author_role |
author |
| author2 |
Costa-Gouveia, J. Vieira de Castro, J. Sotelo, C. G. Vázquez, J. A. Pérez Martín, R. I. Torrado, E. Neves, N. M. Reis, R. L. Castro, A. G. Silva, Tiago H. |
| author2_role |
author author author author author author author author author author |
| dc.contributor.none.fl_str_mv |
Universidade do Minho |
| dc.contributor.author.fl_str_mv |
Alves, A. L. Costa-Gouveia, J. Vieira de Castro, J. Sotelo, C. G. Vázquez, J. A. Pérez Martín, R. I. Torrado, E. Neves, N. M. Reis, R. L. Castro, A. G. Silva, Tiago H. |
| dc.subject.por.fl_str_mv |
Anti-inflammatory macrophages immunogenicity M2-like macrophages marine biomaterials type I collagen Science & Technology |
| topic |
Anti-inflammatory macrophages immunogenicity M2-like macrophages marine biomaterials type I collagen Science & Technology |
| description |
Supplementary material associated with this article can be found, in the online version, at doi: 10.1016/j.actbio.2022.01.009 . |
| publishDate |
2022 |
| dc.date.none.fl_str_mv |
2022-01 2022-01-01T00:00:00Z |
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info:eu-repo/semantics/publishedVersion |
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info:eu-repo/semantics/article |
| format |
article |
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publishedVersion |
| dc.identifier.uri.fl_str_mv |
https://hdl.handle.net/1822/76392 |
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https://hdl.handle.net/1822/76392 |
| dc.language.iso.fl_str_mv |
eng |
| language |
eng |
| dc.relation.none.fl_str_mv |
Alves A. L., Costa-Gouveia J., Vieira de Castro J., Sotelo C. G., Vázquez J. A., Pérez Martín R. I., Torrado E., Neves N. M., Reis R. L., Castro A. G., Silva T. H. Study of the immunologic response of marine-derived collagen and gelatin extracts for tissue engineering applications, Acta Biomaterialia, Vol. 141, pp. 123-131, doi:10.1016/j.actbio.2022.01.009, 2022 1742-7061 10.1016/j.actbio.2022.01.009 35017072 https://doi.org/10.1016/j.actbio.2022.01.009 |
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info:eu-repo/semantics/openAccess |
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openAccess |
| dc.format.none.fl_str_mv |
application/pdf |
| dc.publisher.none.fl_str_mv |
Elsevier 1 |
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Elsevier 1 |
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reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
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Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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RCAAP |
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RCAAP |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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mluisa.alvim@gmail.com |
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1817545186853519360 |