Chitosan-collagen scaffolds can regulate the biological activities of adipose mesenchymal stem cells for tissue engineering

Detalhes bibliográficos
Autor(a) principal: Zotarelli Filho, Idiberto José [UNESP]
Data de Publicação: 2013
Outros Autores: Frascino, Luiz Fernando, Greco, Oswaldo Tadeu, Araújo, José Dalmo de, Bilaqui, Aldemir, Bonilla-Rodriguez, Gustavo Orlando [UNESP]
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://www.hoajonline.com/jrmte/2050-1218/2/12
http://hdl.handle.net/11449/122571
Resumo: Scaffolds of chitosan and collagen can offer a biological niche for the growth of adipose derived stem cells (ADSC). The objective of this work was to characterize the physico-chemical properties of the scaffolds and the ADSC, as well as their interactions to direct influences of the scaffolds on the behavior of ADSC. The methodology included an enzymatic treatment of fat obtained by liposuction by collagenase, ASDC immunophenotyping, cell growth kinetics, biocompatibility studies of the scaffolds analyzed by the activity of alkaline phosphatase (AP), nitric oxide (NO) determination by the Griess-Saltzman reaction, and images of both optical and scanning electron microscopy of the matrices. The extent of the crosslinking of genipin and glutaraldehyde was evaluated by ninhydrin assays, solubility tests and degradation of the matrices. The results showed that the matrices are biocompatible, exhibit physical and chemical properties needed to house cells in vivo and are strong stimulators of signaling proteins (AP) and other molecules (NO) which are important in tissue healing. Therefore, the matrices provide a biological niche for ADSC adhesion, proliferation and cells activities.
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spelling Chitosan-collagen scaffolds can regulate the biological activities of adipose mesenchymal stem cells for tissue engineeringChitosan-collagen-genipin scaffoldsADSCmacrophagesbiological nicheScaffolds of chitosan and collagen can offer a biological niche for the growth of adipose derived stem cells (ADSC). The objective of this work was to characterize the physico-chemical properties of the scaffolds and the ADSC, as well as their interactions to direct influences of the scaffolds on the behavior of ADSC. The methodology included an enzymatic treatment of fat obtained by liposuction by collagenase, ASDC immunophenotyping, cell growth kinetics, biocompatibility studies of the scaffolds analyzed by the activity of alkaline phosphatase (AP), nitric oxide (NO) determination by the Griess-Saltzman reaction, and images of both optical and scanning electron microscopy of the matrices. The extent of the crosslinking of genipin and glutaraldehyde was evaluated by ninhydrin assays, solubility tests and degradation of the matrices. The results showed that the matrices are biocompatible, exhibit physical and chemical properties needed to house cells in vivo and are strong stimulators of signaling proteins (AP) and other molecules (NO) which are important in tissue healing. Therefore, the matrices provide a biological niche for ADSC adhesion, proliferation and cells activities.Universidade Estadual Paulista Júlio de Mesquita Filho, Departamento de Química e Ciências Ambientais, Instituto de Biociências Letras e Ciências Exatas de São José do Rio Preto, São José do Rio Preto, Rua Cristovão Colombo 2265, Jd. Nazaré, CEP 15054000, SP, BrasilUniversidade Estadual Paulista Júlio de Mesquita Filho, Departamento de Química e Ciências Ambientais, Instituto de Biociências Letras e Ciências Exatas de São José do Rio PretoUniversidade Estadual Paulista (Unesp)Instituto de Moléstias Cardiovasculares (IMC)Instituto da BocaZotarelli Filho, Idiberto José [UNESP]Frascino, Luiz FernandoGreco, Oswaldo TadeuAraújo, José Dalmo deBilaqui, AldemirBonilla-Rodriguez, Gustavo Orlando [UNESP]2015-04-27T11:55:52Z2015-04-27T11:55:52Z2013info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article1-10application/pdfhttp://www.hoajonline.com/jrmte/2050-1218/2/12Journal of Regenerative Medicine and Tissue Engineering, v. 2, p. 1-10, 2013.2050-1218http://hdl.handle.net/11449/12257110.7243/2050-1218-2-12ISSN2050-1218-2013-02-01-10.pdf6955258588672130Currículo Lattesreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengjournal of Regenerative Medicine and Tissue Engineeringinfo:eu-repo/semantics/openAccess2023-10-15T06:04:12Zoai:repositorio.unesp.br:11449/122571Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T14:57:37.434887Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Chitosan-collagen scaffolds can regulate the biological activities of adipose mesenchymal stem cells for tissue engineering
title Chitosan-collagen scaffolds can regulate the biological activities of adipose mesenchymal stem cells for tissue engineering
spellingShingle Chitosan-collagen scaffolds can regulate the biological activities of adipose mesenchymal stem cells for tissue engineering
Zotarelli Filho, Idiberto José [UNESP]
Chitosan-collagen-genipin scaffolds
ADSC
macrophages
biological niche
title_short Chitosan-collagen scaffolds can regulate the biological activities of adipose mesenchymal stem cells for tissue engineering
title_full Chitosan-collagen scaffolds can regulate the biological activities of adipose mesenchymal stem cells for tissue engineering
title_fullStr Chitosan-collagen scaffolds can regulate the biological activities of adipose mesenchymal stem cells for tissue engineering
title_full_unstemmed Chitosan-collagen scaffolds can regulate the biological activities of adipose mesenchymal stem cells for tissue engineering
title_sort Chitosan-collagen scaffolds can regulate the biological activities of adipose mesenchymal stem cells for tissue engineering
author Zotarelli Filho, Idiberto José [UNESP]
author_facet Zotarelli Filho, Idiberto José [UNESP]
Frascino, Luiz Fernando
Greco, Oswaldo Tadeu
Araújo, José Dalmo de
Bilaqui, Aldemir
Bonilla-Rodriguez, Gustavo Orlando [UNESP]
author_role author
author2 Frascino, Luiz Fernando
Greco, Oswaldo Tadeu
Araújo, José Dalmo de
Bilaqui, Aldemir
Bonilla-Rodriguez, Gustavo Orlando [UNESP]
author2_role author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade Estadual Paulista (Unesp)
Instituto de Moléstias Cardiovasculares (IMC)
Instituto da Boca
dc.contributor.author.fl_str_mv Zotarelli Filho, Idiberto José [UNESP]
Frascino, Luiz Fernando
Greco, Oswaldo Tadeu
Araújo, José Dalmo de
Bilaqui, Aldemir
Bonilla-Rodriguez, Gustavo Orlando [UNESP]
dc.subject.por.fl_str_mv Chitosan-collagen-genipin scaffolds
ADSC
macrophages
biological niche
topic Chitosan-collagen-genipin scaffolds
ADSC
macrophages
biological niche
description Scaffolds of chitosan and collagen can offer a biological niche for the growth of adipose derived stem cells (ADSC). The objective of this work was to characterize the physico-chemical properties of the scaffolds and the ADSC, as well as their interactions to direct influences of the scaffolds on the behavior of ADSC. The methodology included an enzymatic treatment of fat obtained by liposuction by collagenase, ASDC immunophenotyping, cell growth kinetics, biocompatibility studies of the scaffolds analyzed by the activity of alkaline phosphatase (AP), nitric oxide (NO) determination by the Griess-Saltzman reaction, and images of both optical and scanning electron microscopy of the matrices. The extent of the crosslinking of genipin and glutaraldehyde was evaluated by ninhydrin assays, solubility tests and degradation of the matrices. The results showed that the matrices are biocompatible, exhibit physical and chemical properties needed to house cells in vivo and are strong stimulators of signaling proteins (AP) and other molecules (NO) which are important in tissue healing. Therefore, the matrices provide a biological niche for ADSC adhesion, proliferation and cells activities.
publishDate 2013
dc.date.none.fl_str_mv 2013
2015-04-27T11:55:52Z
2015-04-27T11:55:52Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://www.hoajonline.com/jrmte/2050-1218/2/12
Journal of Regenerative Medicine and Tissue Engineering, v. 2, p. 1-10, 2013.
2050-1218
http://hdl.handle.net/11449/122571
10.7243/2050-1218-2-12
ISSN2050-1218-2013-02-01-10.pdf
6955258588672130
url http://www.hoajonline.com/jrmte/2050-1218/2/12
http://hdl.handle.net/11449/122571
identifier_str_mv Journal of Regenerative Medicine and Tissue Engineering, v. 2, p. 1-10, 2013.
2050-1218
10.7243/2050-1218-2-12
ISSN2050-1218-2013-02-01-10.pdf
6955258588672130
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv journal of Regenerative Medicine and Tissue Engineering
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 1-10
application/pdf
dc.source.none.fl_str_mv Currículo Lattes
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv
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