Chitosan-collagen scaffolds can regulate the biological activities of adipose mesenchymal stem cells for tissue engineering
Autor(a) principal: | |
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Data de Publicação: | 2013 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://www.hoajonline.com/jrmte/2050-1218/2/12 http://hdl.handle.net/11449/122571 |
Resumo: | Scaffolds of chitosan and collagen can offer a biological niche for the growth of adipose derived stem cells (ADSC). The objective of this work was to characterize the physico-chemical properties of the scaffolds and the ADSC, as well as their interactions to direct influences of the scaffolds on the behavior of ADSC. The methodology included an enzymatic treatment of fat obtained by liposuction by collagenase, ASDC immunophenotyping, cell growth kinetics, biocompatibility studies of the scaffolds analyzed by the activity of alkaline phosphatase (AP), nitric oxide (NO) determination by the Griess-Saltzman reaction, and images of both optical and scanning electron microscopy of the matrices. The extent of the crosslinking of genipin and glutaraldehyde was evaluated by ninhydrin assays, solubility tests and degradation of the matrices. The results showed that the matrices are biocompatible, exhibit physical and chemical properties needed to house cells in vivo and are strong stimulators of signaling proteins (AP) and other molecules (NO) which are important in tissue healing. Therefore, the matrices provide a biological niche for ADSC adhesion, proliferation and cells activities. |
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Chitosan-collagen scaffolds can regulate the biological activities of adipose mesenchymal stem cells for tissue engineeringChitosan-collagen-genipin scaffoldsADSCmacrophagesbiological nicheScaffolds of chitosan and collagen can offer a biological niche for the growth of adipose derived stem cells (ADSC). The objective of this work was to characterize the physico-chemical properties of the scaffolds and the ADSC, as well as their interactions to direct influences of the scaffolds on the behavior of ADSC. The methodology included an enzymatic treatment of fat obtained by liposuction by collagenase, ASDC immunophenotyping, cell growth kinetics, biocompatibility studies of the scaffolds analyzed by the activity of alkaline phosphatase (AP), nitric oxide (NO) determination by the Griess-Saltzman reaction, and images of both optical and scanning electron microscopy of the matrices. The extent of the crosslinking of genipin and glutaraldehyde was evaluated by ninhydrin assays, solubility tests and degradation of the matrices. The results showed that the matrices are biocompatible, exhibit physical and chemical properties needed to house cells in vivo and are strong stimulators of signaling proteins (AP) and other molecules (NO) which are important in tissue healing. Therefore, the matrices provide a biological niche for ADSC adhesion, proliferation and cells activities.Universidade Estadual Paulista Júlio de Mesquita Filho, Departamento de Química e Ciências Ambientais, Instituto de Biociências Letras e Ciências Exatas de São José do Rio Preto, São José do Rio Preto, Rua Cristovão Colombo 2265, Jd. Nazaré, CEP 15054000, SP, BrasilUniversidade Estadual Paulista Júlio de Mesquita Filho, Departamento de Química e Ciências Ambientais, Instituto de Biociências Letras e Ciências Exatas de São José do Rio PretoUniversidade Estadual Paulista (Unesp)Instituto de Moléstias Cardiovasculares (IMC)Instituto da BocaZotarelli Filho, Idiberto José [UNESP]Frascino, Luiz FernandoGreco, Oswaldo TadeuAraújo, José Dalmo deBilaqui, AldemirBonilla-Rodriguez, Gustavo Orlando [UNESP]2015-04-27T11:55:52Z2015-04-27T11:55:52Z2013info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article1-10application/pdfhttp://www.hoajonline.com/jrmte/2050-1218/2/12Journal of Regenerative Medicine and Tissue Engineering, v. 2, p. 1-10, 2013.2050-1218http://hdl.handle.net/11449/12257110.7243/2050-1218-2-12ISSN2050-1218-2013-02-01-10.pdf6955258588672130Currículo Lattesreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengjournal of Regenerative Medicine and Tissue Engineeringinfo:eu-repo/semantics/openAccess2023-10-15T06:04:12Zoai:repositorio.unesp.br:11449/122571Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T14:57:37.434887Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Chitosan-collagen scaffolds can regulate the biological activities of adipose mesenchymal stem cells for tissue engineering |
title |
Chitosan-collagen scaffolds can regulate the biological activities of adipose mesenchymal stem cells for tissue engineering |
spellingShingle |
Chitosan-collagen scaffolds can regulate the biological activities of adipose mesenchymal stem cells for tissue engineering Zotarelli Filho, Idiberto José [UNESP] Chitosan-collagen-genipin scaffolds ADSC macrophages biological niche |
title_short |
Chitosan-collagen scaffolds can regulate the biological activities of adipose mesenchymal stem cells for tissue engineering |
title_full |
Chitosan-collagen scaffolds can regulate the biological activities of adipose mesenchymal stem cells for tissue engineering |
title_fullStr |
Chitosan-collagen scaffolds can regulate the biological activities of adipose mesenchymal stem cells for tissue engineering |
title_full_unstemmed |
Chitosan-collagen scaffolds can regulate the biological activities of adipose mesenchymal stem cells for tissue engineering |
title_sort |
Chitosan-collagen scaffolds can regulate the biological activities of adipose mesenchymal stem cells for tissue engineering |
author |
Zotarelli Filho, Idiberto José [UNESP] |
author_facet |
Zotarelli Filho, Idiberto José [UNESP] Frascino, Luiz Fernando Greco, Oswaldo Tadeu Araújo, José Dalmo de Bilaqui, Aldemir Bonilla-Rodriguez, Gustavo Orlando [UNESP] |
author_role |
author |
author2 |
Frascino, Luiz Fernando Greco, Oswaldo Tadeu Araújo, José Dalmo de Bilaqui, Aldemir Bonilla-Rodriguez, Gustavo Orlando [UNESP] |
author2_role |
author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) Instituto de Moléstias Cardiovasculares (IMC) Instituto da Boca |
dc.contributor.author.fl_str_mv |
Zotarelli Filho, Idiberto José [UNESP] Frascino, Luiz Fernando Greco, Oswaldo Tadeu Araújo, José Dalmo de Bilaqui, Aldemir Bonilla-Rodriguez, Gustavo Orlando [UNESP] |
dc.subject.por.fl_str_mv |
Chitosan-collagen-genipin scaffolds ADSC macrophages biological niche |
topic |
Chitosan-collagen-genipin scaffolds ADSC macrophages biological niche |
description |
Scaffolds of chitosan and collagen can offer a biological niche for the growth of adipose derived stem cells (ADSC). The objective of this work was to characterize the physico-chemical properties of the scaffolds and the ADSC, as well as their interactions to direct influences of the scaffolds on the behavior of ADSC. The methodology included an enzymatic treatment of fat obtained by liposuction by collagenase, ASDC immunophenotyping, cell growth kinetics, biocompatibility studies of the scaffolds analyzed by the activity of alkaline phosphatase (AP), nitric oxide (NO) determination by the Griess-Saltzman reaction, and images of both optical and scanning electron microscopy of the matrices. The extent of the crosslinking of genipin and glutaraldehyde was evaluated by ninhydrin assays, solubility tests and degradation of the matrices. The results showed that the matrices are biocompatible, exhibit physical and chemical properties needed to house cells in vivo and are strong stimulators of signaling proteins (AP) and other molecules (NO) which are important in tissue healing. Therefore, the matrices provide a biological niche for ADSC adhesion, proliferation and cells activities. |
publishDate |
2013 |
dc.date.none.fl_str_mv |
2013 2015-04-27T11:55:52Z 2015-04-27T11:55:52Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://www.hoajonline.com/jrmte/2050-1218/2/12 Journal of Regenerative Medicine and Tissue Engineering, v. 2, p. 1-10, 2013. 2050-1218 http://hdl.handle.net/11449/122571 10.7243/2050-1218-2-12 ISSN2050-1218-2013-02-01-10.pdf 6955258588672130 |
url |
http://www.hoajonline.com/jrmte/2050-1218/2/12 http://hdl.handle.net/11449/122571 |
identifier_str_mv |
Journal of Regenerative Medicine and Tissue Engineering, v. 2, p. 1-10, 2013. 2050-1218 10.7243/2050-1218-2-12 ISSN2050-1218-2013-02-01-10.pdf 6955258588672130 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
journal of Regenerative Medicine and Tissue Engineering |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
1-10 application/pdf |
dc.source.none.fl_str_mv |
Currículo Lattes reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1808128441709494272 |