Identification of the human Lewisa carbohydrate motif in a secretory peroxidase from a plant cell suspension culture (Vaccinium myrtillus L.)

Detalhes bibliográficos
Autor(a) principal: Melo, Nuno
Data de Publicação: 1997
Outros Autores: Nimtz, Manfred, Conradt, Harald S, Fevereiro, Pedro S, Costa, Júlia
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10400.21/14237
Resumo: This paper reports for the first time the presence of the human Lewisa type determinant in glycoproteins secreted by plant cells. A single glycopeptide was identified in the tryptic hydrolysis of the peroxidase VMPxCl from Vaccinium myrtillus L. by HPLC/ESI-MS. The oligosaccharide structures were elucidated by ESI-MS-MS and by methylation analysis before and after removal of fucose by mild acid hydrolysis.
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spelling Identification of the human Lewisa carbohydrate motif in a secretory peroxidase from a plant cell suspension culture (Vaccinium myrtillus L.)Extracellular glycoproteinPeroxidasePlant complex oligosaccharideVaccinium myrtilluThis paper reports for the first time the presence of the human Lewisa type determinant in glycoproteins secreted by plant cells. A single glycopeptide was identified in the tryptic hydrolysis of the peroxidase VMPxCl from Vaccinium myrtillus L. by HPLC/ESI-MS. The oligosaccharide structures were elucidated by ESI-MS-MS and by methylation analysis before and after removal of fucose by mild acid hydrolysis.RCIPLMelo, NunoNimtz, ManfredConradt, Harald SFevereiro, Pedro SCosta, Júlia2022-02-02T14:31:55Z19971997-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10400.21/14237engMelo, Nuno S, Nimtz, Manfred, Conradt, Harald S, Fevereiro, Pedro S and Costa, Júlia(1997), Identification of the human Lewisa carbohydrate motif in a secretory peroxidase from a plant cell suspension culture (Vaccinium myrtillus L.), FEBS Letters, 415, doi: 10.1016/S0014-5793(97)01121-60014-579310.1016/S0014-5793(97)01121-6info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-08-03T10:10:07Zoai:repositorio.ipl.pt:10400.21/14237Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T20:22:04.794396Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Identification of the human Lewisa carbohydrate motif in a secretory peroxidase from a plant cell suspension culture (Vaccinium myrtillus L.)
title Identification of the human Lewisa carbohydrate motif in a secretory peroxidase from a plant cell suspension culture (Vaccinium myrtillus L.)
spellingShingle Identification of the human Lewisa carbohydrate motif in a secretory peroxidase from a plant cell suspension culture (Vaccinium myrtillus L.)
Melo, Nuno
Extracellular glycoprotein
Peroxidase
Plant complex oligosaccharide
Vaccinium myrtillu
title_short Identification of the human Lewisa carbohydrate motif in a secretory peroxidase from a plant cell suspension culture (Vaccinium myrtillus L.)
title_full Identification of the human Lewisa carbohydrate motif in a secretory peroxidase from a plant cell suspension culture (Vaccinium myrtillus L.)
title_fullStr Identification of the human Lewisa carbohydrate motif in a secretory peroxidase from a plant cell suspension culture (Vaccinium myrtillus L.)
title_full_unstemmed Identification of the human Lewisa carbohydrate motif in a secretory peroxidase from a plant cell suspension culture (Vaccinium myrtillus L.)
title_sort Identification of the human Lewisa carbohydrate motif in a secretory peroxidase from a plant cell suspension culture (Vaccinium myrtillus L.)
author Melo, Nuno
author_facet Melo, Nuno
Nimtz, Manfred
Conradt, Harald S
Fevereiro, Pedro S
Costa, Júlia
author_role author
author2 Nimtz, Manfred
Conradt, Harald S
Fevereiro, Pedro S
Costa, Júlia
author2_role author
author
author
author
dc.contributor.none.fl_str_mv RCIPL
dc.contributor.author.fl_str_mv Melo, Nuno
Nimtz, Manfred
Conradt, Harald S
Fevereiro, Pedro S
Costa, Júlia
dc.subject.por.fl_str_mv Extracellular glycoprotein
Peroxidase
Plant complex oligosaccharide
Vaccinium myrtillu
topic Extracellular glycoprotein
Peroxidase
Plant complex oligosaccharide
Vaccinium myrtillu
description This paper reports for the first time the presence of the human Lewisa type determinant in glycoproteins secreted by plant cells. A single glycopeptide was identified in the tryptic hydrolysis of the peroxidase VMPxCl from Vaccinium myrtillus L. by HPLC/ESI-MS. The oligosaccharide structures were elucidated by ESI-MS-MS and by methylation analysis before and after removal of fucose by mild acid hydrolysis.
publishDate 1997
dc.date.none.fl_str_mv 1997
1997-01-01T00:00:00Z
2022-02-02T14:31:55Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10400.21/14237
url http://hdl.handle.net/10400.21/14237
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Melo, Nuno S, Nimtz, Manfred, Conradt, Harald S, Fevereiro, Pedro S and Costa, Júlia(1997), Identification of the human Lewisa carbohydrate motif in a secretory peroxidase from a plant cell suspension culture (Vaccinium myrtillus L.), FEBS Letters, 415, doi: 10.1016/S0014-5793(97)01121-6
0014-5793
10.1016/S0014-5793(97)01121-6
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
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reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
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