Bacteriophages as a smart tool to target Alzheimer's Disease

Detalhes bibliográficos
Autor(a) principal: Martins, Ivone M.
Data de Publicação: 2019
Outros Autores: Resende, Mariana, Silva, Ana Isabel M., Lodder, Tessa, Cristóvão, Joana, Gomes, Cláudio M., Azeredo, Joana, Kessels, Helmut W.
Tipo de documento: Artigo de conferência
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/1822/70225
Resumo: PHAGES 2019 - Bacteriophage in Medicine, Food and Biotechnology
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spelling Bacteriophages as a smart tool to target Alzheimer's DiseasePHAGES 2019 - Bacteriophage in Medicine, Food and BiotechnologyAlzheimer's disease is an irreversible, progressive and age-dependent neurodegenerative disorder affecting millions of people worldwide. In our brain, a small sized amyloid-beta (Abeta) peptide, when in excess, progressively aggregates into soluble oligomers and fibrils, to finally deposit and accumulate as insoluble amyloid plaques. The still-soluble Abeta oligomers and fibrils are sufficient to corrupt synaptic function and trigger memory loss, emphasizing the importance to find diagnostic tools targeting the soluble forms of Abeta. Specific amyloidogenic motifs were described to react with AB fibrils: AB30-39, and with fibrils and oligomers: AB33-42. However, the blood-brain-barrier (BBB), is a major bottleneck for effective brain applications. To overcome this limitation, bacteriophages (phages) and phage display technology may represent an interesting tool as delivery vehicles to shuttle its cargo across this BBB. To examine this possibility, peptides AB30-39 and AB33-42 were cloned into the M13 filamentous phage genome, and the binding ability of AB phages towards Abeta-oligomers and fibrils was tested in vitro. Our results show that these Abeta-specific phages inhibit oligomers and fibril formation as well as promote fibril disaggregation. Moreover, results revealed the specificity of recognition of each engineered phage. AB-phages were also tested in vivo to characterize their biodistribution in disease mouse models and preliminary results suggest that AB30-39 is able to recognize amyloid-beta in the brain of these animals. For future work, this system will be tested to assess its therapeutic potential to restore the synaptic function and memory formation. This approach may provide phage-based tools for Alzheimer's early diagnose and therapy.info:eu-repo/semantics/publishedVersionUniversidade do MinhoMartins, Ivone M.Resende, MarianaSilva, Ana Isabel M.Lodder, TessaCristóvão, JoanaGomes, Cláudio M.Azeredo, JoanaKessels, Helmut W.2019-09-112019-09-11T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/conferenceObjectapplication/pdfhttp://hdl.handle.net/1822/70225engMartins, Ivone M.; Resende, Mariana; Silva, Ana Isabel M.; Lodder, T.; Cristóvão, J.; Gomes, C.; Azeredo, Joana; Kessels, H., Bacteriophages as a smart tool to target Alzheimer's Disease. PHAGES 2019 - Bacteriophage in Medicine, Food and Biotechnology, Oxford, United Kingdom, 2019.info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-21T12:45:35Zoai:repositorium.sdum.uminho.pt:1822/70225Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T19:43:27.019474Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Bacteriophages as a smart tool to target Alzheimer's Disease
title Bacteriophages as a smart tool to target Alzheimer's Disease
spellingShingle Bacteriophages as a smart tool to target Alzheimer's Disease
Martins, Ivone M.
title_short Bacteriophages as a smart tool to target Alzheimer's Disease
title_full Bacteriophages as a smart tool to target Alzheimer's Disease
title_fullStr Bacteriophages as a smart tool to target Alzheimer's Disease
title_full_unstemmed Bacteriophages as a smart tool to target Alzheimer's Disease
title_sort Bacteriophages as a smart tool to target Alzheimer's Disease
author Martins, Ivone M.
author_facet Martins, Ivone M.
Resende, Mariana
Silva, Ana Isabel M.
Lodder, Tessa
Cristóvão, Joana
Gomes, Cláudio M.
Azeredo, Joana
Kessels, Helmut W.
author_role author
author2 Resende, Mariana
Silva, Ana Isabel M.
Lodder, Tessa
Cristóvão, Joana
Gomes, Cláudio M.
Azeredo, Joana
Kessels, Helmut W.
author2_role author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade do Minho
dc.contributor.author.fl_str_mv Martins, Ivone M.
Resende, Mariana
Silva, Ana Isabel M.
Lodder, Tessa
Cristóvão, Joana
Gomes, Cláudio M.
Azeredo, Joana
Kessels, Helmut W.
description PHAGES 2019 - Bacteriophage in Medicine, Food and Biotechnology
publishDate 2019
dc.date.none.fl_str_mv 2019-09-11
2019-09-11T00:00:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/conferenceObject
format conferenceObject
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/1822/70225
url http://hdl.handle.net/1822/70225
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Martins, Ivone M.; Resende, Mariana; Silva, Ana Isabel M.; Lodder, T.; Cristóvão, J.; Gomes, C.; Azeredo, Joana; Kessels, H., Bacteriophages as a smart tool to target Alzheimer's Disease. PHAGES 2019 - Bacteriophage in Medicine, Food and Biotechnology, Oxford, United Kingdom, 2019.
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
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reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
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