Bacteriophages as a smart tool to target Alzheimer's Disease
Autor(a) principal: | |
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Data de Publicação: | 2019 |
Outros Autores: | , , , , , , |
Tipo de documento: | Artigo de conferência |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/1822/70225 |
Resumo: | PHAGES 2019 - Bacteriophage in Medicine, Food and Biotechnology |
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Bacteriophages as a smart tool to target Alzheimer's DiseasePHAGES 2019 - Bacteriophage in Medicine, Food and BiotechnologyAlzheimer's disease is an irreversible, progressive and age-dependent neurodegenerative disorder affecting millions of people worldwide. In our brain, a small sized amyloid-beta (Abeta) peptide, when in excess, progressively aggregates into soluble oligomers and fibrils, to finally deposit and accumulate as insoluble amyloid plaques. The still-soluble Abeta oligomers and fibrils are sufficient to corrupt synaptic function and trigger memory loss, emphasizing the importance to find diagnostic tools targeting the soluble forms of Abeta. Specific amyloidogenic motifs were described to react with AB fibrils: AB30-39, and with fibrils and oligomers: AB33-42. However, the blood-brain-barrier (BBB), is a major bottleneck for effective brain applications. To overcome this limitation, bacteriophages (phages) and phage display technology may represent an interesting tool as delivery vehicles to shuttle its cargo across this BBB. To examine this possibility, peptides AB30-39 and AB33-42 were cloned into the M13 filamentous phage genome, and the binding ability of AB phages towards Abeta-oligomers and fibrils was tested in vitro. Our results show that these Abeta-specific phages inhibit oligomers and fibril formation as well as promote fibril disaggregation. Moreover, results revealed the specificity of recognition of each engineered phage. AB-phages were also tested in vivo to characterize their biodistribution in disease mouse models and preliminary results suggest that AB30-39 is able to recognize amyloid-beta in the brain of these animals. For future work, this system will be tested to assess its therapeutic potential to restore the synaptic function and memory formation. This approach may provide phage-based tools for Alzheimer's early diagnose and therapy.info:eu-repo/semantics/publishedVersionUniversidade do MinhoMartins, Ivone M.Resende, MarianaSilva, Ana Isabel M.Lodder, TessaCristóvão, JoanaGomes, Cláudio M.Azeredo, JoanaKessels, Helmut W.2019-09-112019-09-11T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/conferenceObjectapplication/pdfhttp://hdl.handle.net/1822/70225engMartins, Ivone M.; Resende, Mariana; Silva, Ana Isabel M.; Lodder, T.; Cristóvão, J.; Gomes, C.; Azeredo, Joana; Kessels, H., Bacteriophages as a smart tool to target Alzheimer's Disease. PHAGES 2019 - Bacteriophage in Medicine, Food and Biotechnology, Oxford, United Kingdom, 2019.info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-21T12:45:35Zoai:repositorium.sdum.uminho.pt:1822/70225Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T19:43:27.019474Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Bacteriophages as a smart tool to target Alzheimer's Disease |
title |
Bacteriophages as a smart tool to target Alzheimer's Disease |
spellingShingle |
Bacteriophages as a smart tool to target Alzheimer's Disease Martins, Ivone M. |
title_short |
Bacteriophages as a smart tool to target Alzheimer's Disease |
title_full |
Bacteriophages as a smart tool to target Alzheimer's Disease |
title_fullStr |
Bacteriophages as a smart tool to target Alzheimer's Disease |
title_full_unstemmed |
Bacteriophages as a smart tool to target Alzheimer's Disease |
title_sort |
Bacteriophages as a smart tool to target Alzheimer's Disease |
author |
Martins, Ivone M. |
author_facet |
Martins, Ivone M. Resende, Mariana Silva, Ana Isabel M. Lodder, Tessa Cristóvão, Joana Gomes, Cláudio M. Azeredo, Joana Kessels, Helmut W. |
author_role |
author |
author2 |
Resende, Mariana Silva, Ana Isabel M. Lodder, Tessa Cristóvão, Joana Gomes, Cláudio M. Azeredo, Joana Kessels, Helmut W. |
author2_role |
author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade do Minho |
dc.contributor.author.fl_str_mv |
Martins, Ivone M. Resende, Mariana Silva, Ana Isabel M. Lodder, Tessa Cristóvão, Joana Gomes, Cláudio M. Azeredo, Joana Kessels, Helmut W. |
description |
PHAGES 2019 - Bacteriophage in Medicine, Food and Biotechnology |
publishDate |
2019 |
dc.date.none.fl_str_mv |
2019-09-11 2019-09-11T00:00:00Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
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info:eu-repo/semantics/conferenceObject |
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conferenceObject |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/1822/70225 |
url |
http://hdl.handle.net/1822/70225 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Martins, Ivone M.; Resende, Mariana; Silva, Ana Isabel M.; Lodder, T.; Cristóvão, J.; Gomes, C.; Azeredo, Joana; Kessels, H., Bacteriophages as a smart tool to target Alzheimer's Disease. PHAGES 2019 - Bacteriophage in Medicine, Food and Biotechnology, Oxford, United Kingdom, 2019. |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
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Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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