Bubbles moving in blood flow in a microchannel network: the effect on the local hematocrit
Autor(a) principal: | |
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Data de Publicação: | 2020 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/1822/65144 |
Resumo: | Air inside of blood vessels is a phenomenon known as gas embolism. During the past years, studies have been performed to assess the influence of air bubbles in microcirculation. In this study, we investigated the flow of bubbles in a microchannel network with several bifurcations, mimicking part of a capillary system. Thus, two working fluids were used, composed by sheep red blood cells (RBCs) suspended in a Dextran 40 solution with different hematocrits (5% and 10%). The experiments were carried out in a polydimethylsiloxane (PDMS) microchannel network fabricated by a soft lithography. A high-speed video microscopy system was used to obtain the results for a blood flow rate of 10 µL/min. This system enables the visualization of bubble formation and flow along the network. The results showed that the passage of air bubbles strongly influences the cell’s local concentration, since a higher concentration of cells was observed upstream of the bubble, whereas a lower local hematocrit was visualized at the region downstream of the bubble. In bifurcations, bubbles may split asymmetrically, leading to an uneven distribution of RBCs between the outflow branches. |
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Bubbles moving in blood flow in a microchannel network: the effect on the local hematocritblood flowgas embolismmicrocirculationbifurcationsnetworkScience & TechnologyAir inside of blood vessels is a phenomenon known as gas embolism. During the past years, studies have been performed to assess the influence of air bubbles in microcirculation. In this study, we investigated the flow of bubbles in a microchannel network with several bifurcations, mimicking part of a capillary system. Thus, two working fluids were used, composed by sheep red blood cells (RBCs) suspended in a Dextran 40 solution with different hematocrits (5% and 10%). The experiments were carried out in a polydimethylsiloxane (PDMS) microchannel network fabricated by a soft lithography. A high-speed video microscopy system was used to obtain the results for a blood flow rate of 10 µL/min. This system enables the visualization of bubble formation and flow along the network. The results showed that the passage of air bubbles strongly influences the cell’s local concentration, since a higher concentration of cells was observed upstream of the bubble, whereas a lower local hematocrit was visualized at the region downstream of the bubble. In bifurcations, bubbles may split asymmetrically, leading to an uneven distribution of RBCs between the outflow branches.This research was funded by Portuguese national funds of FCT/MCTES (PIDDAC) through the base funding from the following research units: UIDB/00532/2020 (Transport Phenomena Research Center—CEFT). UIDB/04077/2020 (Research Center (Mechanical Engineering and Resource Sustainability Center—MEtRICs). The authors are also grateful for the partial funding of FCT through the projects PTDC/SAU-ENB/116929/2010, POCI-01-0145-FEDER-016861 (ref: PTDC/QEQ-FTT/4287/2014), NORTE-01-0145-FEDER-029394 and NORTE-01-0145-FEDER-030171 funded by COMPETE2020, NORTE2020. PORTUGAL2020 and FEDER. D. Bento acknowledges the PhD scholarship SFRH/BD/91192/2012 granted by FCT.Multidisciplinary Digital Publishing InstituteUniversidade do MinhoBento, DavidLopes, SaraMaia, InêsLima, Rui Alberto Madeira MacedoMiranda, João M.2020-03-262020-03-26T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/1822/65144engBento, D.; Lopes, S.; Maia, I.; Lima, R.; Miranda, J.M. Bubbles Moving in Blood Flow in a Microchannel Network: The Effect on the Local Hematocrit. Micromachines 2020, 11, 344.2072-666X10.3390/mi11040344https://www.mdpi.com/2072-666X/11/4/344info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-21T11:57:40Zoai:repositorium.sdum.uminho.pt:1822/65144Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T18:47:21.559473Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Bubbles moving in blood flow in a microchannel network: the effect on the local hematocrit |
title |
Bubbles moving in blood flow in a microchannel network: the effect on the local hematocrit |
spellingShingle |
Bubbles moving in blood flow in a microchannel network: the effect on the local hematocrit Bento, David blood flow gas embolism microcirculation bifurcations network Science & Technology |
title_short |
Bubbles moving in blood flow in a microchannel network: the effect on the local hematocrit |
title_full |
Bubbles moving in blood flow in a microchannel network: the effect on the local hematocrit |
title_fullStr |
Bubbles moving in blood flow in a microchannel network: the effect on the local hematocrit |
title_full_unstemmed |
Bubbles moving in blood flow in a microchannel network: the effect on the local hematocrit |
title_sort |
Bubbles moving in blood flow in a microchannel network: the effect on the local hematocrit |
author |
Bento, David |
author_facet |
Bento, David Lopes, Sara Maia, Inês Lima, Rui Alberto Madeira Macedo Miranda, João M. |
author_role |
author |
author2 |
Lopes, Sara Maia, Inês Lima, Rui Alberto Madeira Macedo Miranda, João M. |
author2_role |
author author author author |
dc.contributor.none.fl_str_mv |
Universidade do Minho |
dc.contributor.author.fl_str_mv |
Bento, David Lopes, Sara Maia, Inês Lima, Rui Alberto Madeira Macedo Miranda, João M. |
dc.subject.por.fl_str_mv |
blood flow gas embolism microcirculation bifurcations network Science & Technology |
topic |
blood flow gas embolism microcirculation bifurcations network Science & Technology |
description |
Air inside of blood vessels is a phenomenon known as gas embolism. During the past years, studies have been performed to assess the influence of air bubbles in microcirculation. In this study, we investigated the flow of bubbles in a microchannel network with several bifurcations, mimicking part of a capillary system. Thus, two working fluids were used, composed by sheep red blood cells (RBCs) suspended in a Dextran 40 solution with different hematocrits (5% and 10%). The experiments were carried out in a polydimethylsiloxane (PDMS) microchannel network fabricated by a soft lithography. A high-speed video microscopy system was used to obtain the results for a blood flow rate of 10 µL/min. This system enables the visualization of bubble formation and flow along the network. The results showed that the passage of air bubbles strongly influences the cell’s local concentration, since a higher concentration of cells was observed upstream of the bubble, whereas a lower local hematocrit was visualized at the region downstream of the bubble. In bifurcations, bubbles may split asymmetrically, leading to an uneven distribution of RBCs between the outflow branches. |
publishDate |
2020 |
dc.date.none.fl_str_mv |
2020-03-26 2020-03-26T00:00:00Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/1822/65144 |
url |
http://hdl.handle.net/1822/65144 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Bento, D.; Lopes, S.; Maia, I.; Lima, R.; Miranda, J.M. Bubbles Moving in Blood Flow in a Microchannel Network: The Effect on the Local Hematocrit. Micromachines 2020, 11, 344. 2072-666X 10.3390/mi11040344 https://www.mdpi.com/2072-666X/11/4/344 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Multidisciplinary Digital Publishing Institute |
publisher.none.fl_str_mv |
Multidisciplinary Digital Publishing Institute |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
instname_str |
Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
instacron_str |
RCAAP |
institution |
RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
collection |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository.name.fl_str_mv |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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1799132231273283584 |