Delivery of Oligonucleotides into Bacteria by Fusogenic Liposomes

Detalhes bibliográficos
Autor(a) principal: Sara Pereira
Data de Publicação: 2021
Outros Autores: Rita S. Santos, Luís Moreira, Nuno M. Guimarães, Kevin Braeckmans, Stefaan C. De Smedt, Nuno F. Azevedo
Tipo de documento: Livro
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: https://hdl.handle.net/10216/140770
Resumo: Oligonucleotides able to hybridize bacterial RNA via in situ hybridization may potentially act as new antimicrobials, replacing antibiotics, and as fast in vivo diagnostic probes, outperforming current clinical methodologies. Nonetheless, oligonucleotides are not able to efficiently permeate the multi-layered bacterial envelope to reach their target RNA in the cytosol. Cationic fusogenic liposomes are here suggested as vehicles to enable the internalization of oligonucleotides in bacteria. Here, we describe the formulation of DOTAP-DOPE liposomes, their complexation with small negatively charged oligonucleotides, and the evaluation of the intracellular delivery of the oligonucleotides in bacteria. This strategy uncovers the potential of performing FISH in vivo for real-time detection and treatment of infections. (c) 2020, Springer Science+Business Media, LLC, part of Springer Nature.
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spelling Delivery of Oligonucleotides into Bacteria by Fusogenic LiposomesOligonucleotides able to hybridize bacterial RNA via in situ hybridization may potentially act as new antimicrobials, replacing antibiotics, and as fast in vivo diagnostic probes, outperforming current clinical methodologies. Nonetheless, oligonucleotides are not able to efficiently permeate the multi-layered bacterial envelope to reach their target RNA in the cytosol. Cationic fusogenic liposomes are here suggested as vehicles to enable the internalization of oligonucleotides in bacteria. Here, we describe the formulation of DOTAP-DOPE liposomes, their complexation with small negatively charged oligonucleotides, and the evaluation of the intracellular delivery of the oligonucleotides in bacteria. This strategy uncovers the potential of performing FISH in vivo for real-time detection and treatment of infections. (c) 2020, Springer Science+Business Media, LLC, part of Springer Nature.2021-02-132021-02-13T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/bookapplication/pdfhttps://hdl.handle.net/10216/140770eng10.1007/978-1-0716-1115-9_6Sara PereiraRita S. SantosLuís MoreiraNuno M. GuimarãesKevin BraeckmansStefaan C. De SmedtNuno F. Azevedoinfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-11-29T14:41:23Zoai:repositorio-aberto.up.pt:10216/140770Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T00:06:51.375205Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Delivery of Oligonucleotides into Bacteria by Fusogenic Liposomes
title Delivery of Oligonucleotides into Bacteria by Fusogenic Liposomes
spellingShingle Delivery of Oligonucleotides into Bacteria by Fusogenic Liposomes
Sara Pereira
title_short Delivery of Oligonucleotides into Bacteria by Fusogenic Liposomes
title_full Delivery of Oligonucleotides into Bacteria by Fusogenic Liposomes
title_fullStr Delivery of Oligonucleotides into Bacteria by Fusogenic Liposomes
title_full_unstemmed Delivery of Oligonucleotides into Bacteria by Fusogenic Liposomes
title_sort Delivery of Oligonucleotides into Bacteria by Fusogenic Liposomes
author Sara Pereira
author_facet Sara Pereira
Rita S. Santos
Luís Moreira
Nuno M. Guimarães
Kevin Braeckmans
Stefaan C. De Smedt
Nuno F. Azevedo
author_role author
author2 Rita S. Santos
Luís Moreira
Nuno M. Guimarães
Kevin Braeckmans
Stefaan C. De Smedt
Nuno F. Azevedo
author2_role author
author
author
author
author
author
dc.contributor.author.fl_str_mv Sara Pereira
Rita S. Santos
Luís Moreira
Nuno M. Guimarães
Kevin Braeckmans
Stefaan C. De Smedt
Nuno F. Azevedo
description Oligonucleotides able to hybridize bacterial RNA via in situ hybridization may potentially act as new antimicrobials, replacing antibiotics, and as fast in vivo diagnostic probes, outperforming current clinical methodologies. Nonetheless, oligonucleotides are not able to efficiently permeate the multi-layered bacterial envelope to reach their target RNA in the cytosol. Cationic fusogenic liposomes are here suggested as vehicles to enable the internalization of oligonucleotides in bacteria. Here, we describe the formulation of DOTAP-DOPE liposomes, their complexation with small negatively charged oligonucleotides, and the evaluation of the intracellular delivery of the oligonucleotides in bacteria. This strategy uncovers the potential of performing FISH in vivo for real-time detection and treatment of infections. (c) 2020, Springer Science+Business Media, LLC, part of Springer Nature.
publishDate 2021
dc.date.none.fl_str_mv 2021-02-13
2021-02-13T00:00:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/book
format book
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://hdl.handle.net/10216/140770
url https://hdl.handle.net/10216/140770
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1007/978-1-0716-1115-9_6
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
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repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
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