Delivery of Oligonucleotides into Bacteria by Fusogenic Liposomes
Autor(a) principal: | |
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Data de Publicação: | 2021 |
Outros Autores: | , , , , , |
Tipo de documento: | Livro |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | https://hdl.handle.net/10216/140770 |
Resumo: | Oligonucleotides able to hybridize bacterial RNA via in situ hybridization may potentially act as new antimicrobials, replacing antibiotics, and as fast in vivo diagnostic probes, outperforming current clinical methodologies. Nonetheless, oligonucleotides are not able to efficiently permeate the multi-layered bacterial envelope to reach their target RNA in the cytosol. Cationic fusogenic liposomes are here suggested as vehicles to enable the internalization of oligonucleotides in bacteria. Here, we describe the formulation of DOTAP-DOPE liposomes, their complexation with small negatively charged oligonucleotides, and the evaluation of the intracellular delivery of the oligonucleotides in bacteria. This strategy uncovers the potential of performing FISH in vivo for real-time detection and treatment of infections. (c) 2020, Springer Science+Business Media, LLC, part of Springer Nature. |
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Delivery of Oligonucleotides into Bacteria by Fusogenic LiposomesOligonucleotides able to hybridize bacterial RNA via in situ hybridization may potentially act as new antimicrobials, replacing antibiotics, and as fast in vivo diagnostic probes, outperforming current clinical methodologies. Nonetheless, oligonucleotides are not able to efficiently permeate the multi-layered bacterial envelope to reach their target RNA in the cytosol. Cationic fusogenic liposomes are here suggested as vehicles to enable the internalization of oligonucleotides in bacteria. Here, we describe the formulation of DOTAP-DOPE liposomes, their complexation with small negatively charged oligonucleotides, and the evaluation of the intracellular delivery of the oligonucleotides in bacteria. This strategy uncovers the potential of performing FISH in vivo for real-time detection and treatment of infections. (c) 2020, Springer Science+Business Media, LLC, part of Springer Nature.2021-02-132021-02-13T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/bookapplication/pdfhttps://hdl.handle.net/10216/140770eng10.1007/978-1-0716-1115-9_6Sara PereiraRita S. SantosLuís MoreiraNuno M. GuimarãesKevin BraeckmansStefaan C. De SmedtNuno F. Azevedoinfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-11-29T14:41:23Zoai:repositorio-aberto.up.pt:10216/140770Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T00:06:51.375205Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Delivery of Oligonucleotides into Bacteria by Fusogenic Liposomes |
title |
Delivery of Oligonucleotides into Bacteria by Fusogenic Liposomes |
spellingShingle |
Delivery of Oligonucleotides into Bacteria by Fusogenic Liposomes Sara Pereira |
title_short |
Delivery of Oligonucleotides into Bacteria by Fusogenic Liposomes |
title_full |
Delivery of Oligonucleotides into Bacteria by Fusogenic Liposomes |
title_fullStr |
Delivery of Oligonucleotides into Bacteria by Fusogenic Liposomes |
title_full_unstemmed |
Delivery of Oligonucleotides into Bacteria by Fusogenic Liposomes |
title_sort |
Delivery of Oligonucleotides into Bacteria by Fusogenic Liposomes |
author |
Sara Pereira |
author_facet |
Sara Pereira Rita S. Santos Luís Moreira Nuno M. Guimarães Kevin Braeckmans Stefaan C. De Smedt Nuno F. Azevedo |
author_role |
author |
author2 |
Rita S. Santos Luís Moreira Nuno M. Guimarães Kevin Braeckmans Stefaan C. De Smedt Nuno F. Azevedo |
author2_role |
author author author author author author |
dc.contributor.author.fl_str_mv |
Sara Pereira Rita S. Santos Luís Moreira Nuno M. Guimarães Kevin Braeckmans Stefaan C. De Smedt Nuno F. Azevedo |
description |
Oligonucleotides able to hybridize bacterial RNA via in situ hybridization may potentially act as new antimicrobials, replacing antibiotics, and as fast in vivo diagnostic probes, outperforming current clinical methodologies. Nonetheless, oligonucleotides are not able to efficiently permeate the multi-layered bacterial envelope to reach their target RNA in the cytosol. Cationic fusogenic liposomes are here suggested as vehicles to enable the internalization of oligonucleotides in bacteria. Here, we describe the formulation of DOTAP-DOPE liposomes, their complexation with small negatively charged oligonucleotides, and the evaluation of the intracellular delivery of the oligonucleotides in bacteria. This strategy uncovers the potential of performing FISH in vivo for real-time detection and treatment of infections. (c) 2020, Springer Science+Business Media, LLC, part of Springer Nature. |
publishDate |
2021 |
dc.date.none.fl_str_mv |
2021-02-13 2021-02-13T00:00:00Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/book |
format |
book |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://hdl.handle.net/10216/140770 |
url |
https://hdl.handle.net/10216/140770 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1007/978-1-0716-1115-9_6 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.source.none.fl_str_mv |
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Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
instacron_str |
RCAAP |
institution |
RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
collection |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository.name.fl_str_mv |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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1799135993805144064 |