Protein crystallization as a process step in a novel meso oscillatory flow reactor: study of lysozyme phase behavior
Autor(a) principal: | |
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Data de Publicação: | 2016 |
Outros Autores: | , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/1822/42213 |
Resumo: | In the present work, it is reported for the first time the study of the applicability of a novel meso oscillatory flow reactor (meso-OFR) for protein crystallization as a process step. Crystallization assays carried out in the designed device enabled to derive a two-dimensional lysozyme phase diagram (lysozyme concentration against sodium chloride concentration). Results evidence the formation of several types of crystals (different size and shape), with a strong influence of salt concentration on crystal shape. Results also show that lysozyme remains active at the end of the experiments. Furthermore, it was possible to verify the reduction of the metastability zone when compared to lysozyme crystallization conducted under quiescent conditions. Induction times were also measured by online monitoring of the turbidity of the crystallization solution, obtained values being between 41 and 900 minutes. Beyond providing improved understanding of protein phase behavior under oscillatory flow mixing, the results are very promising regarding the feasibility of the designed methodology for protein crystallization as a process step. |
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Protein crystallization as a process step in a novel meso oscillatory flow reactor: study of lysozyme phase behaviorScience & TechnologyIn the present work, it is reported for the first time the study of the applicability of a novel meso oscillatory flow reactor (meso-OFR) for protein crystallization as a process step. Crystallization assays carried out in the designed device enabled to derive a two-dimensional lysozyme phase diagram (lysozyme concentration against sodium chloride concentration). Results evidence the formation of several types of crystals (different size and shape), with a strong influence of salt concentration on crystal shape. Results also show that lysozyme remains active at the end of the experiments. Furthermore, it was possible to verify the reduction of the metastability zone when compared to lysozyme crystallization conducted under quiescent conditions. Induction times were also measured by online monitoring of the turbidity of the crystallization solution, obtained values being between 41 and 900 minutes. Beyond providing improved understanding of protein phase behavior under oscillatory flow mixing, the results are very promising regarding the feasibility of the designed methodology for protein crystallization as a process step.This work was financially supported by the European Regional Development Fund (FEDER) through COMPETE 2020-Operational Programme Competitiveness and Internationalization (POCI) (UID/EQU/00511/2013-LEPABE-Laboratory for Process Engineering, Environment, Biotechnology and Energy-EQU/00511; POCI-01-0145-FEDER-006684) and by national funds through FCT-Portuguese Foundation for Science and Technology-under the projects: UID/BIO/04469/2013; IF exploratory project [IF/01087/2014]; postdoctoral Fellowship [SFRH/BPD/96132/2013]. A. Ferreira is an Investigator FCT (IF/01087/2014).American Chemical SocietyUniversidade do MinhoCastro, FilipaFerreira, AntónioTeixeira, J. A.Rocha, Fernando20162016-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/1822/42213engCastro, Filipa; Ferreira, António; Teixeira, J. A.; Rocha, Fernando, Protein crystallization as a process step in a novel meso oscillatory flow reactor: study of lysozyme phase behavior. Crystal Growth and Design, 16(7), 3748-3755, 20161528-74831528-750510.1021/acs.cgd.6b00262http://pubs.acs.org/journal/cgdefuinfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-21T12:23:42Zoai:repositorium.sdum.uminho.pt:1822/42213Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T19:17:30.404659Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Protein crystallization as a process step in a novel meso oscillatory flow reactor: study of lysozyme phase behavior |
title |
Protein crystallization as a process step in a novel meso oscillatory flow reactor: study of lysozyme phase behavior |
spellingShingle |
Protein crystallization as a process step in a novel meso oscillatory flow reactor: study of lysozyme phase behavior Castro, Filipa Science & Technology |
title_short |
Protein crystallization as a process step in a novel meso oscillatory flow reactor: study of lysozyme phase behavior |
title_full |
Protein crystallization as a process step in a novel meso oscillatory flow reactor: study of lysozyme phase behavior |
title_fullStr |
Protein crystallization as a process step in a novel meso oscillatory flow reactor: study of lysozyme phase behavior |
title_full_unstemmed |
Protein crystallization as a process step in a novel meso oscillatory flow reactor: study of lysozyme phase behavior |
title_sort |
Protein crystallization as a process step in a novel meso oscillatory flow reactor: study of lysozyme phase behavior |
author |
Castro, Filipa |
author_facet |
Castro, Filipa Ferreira, António Teixeira, J. A. Rocha, Fernando |
author_role |
author |
author2 |
Ferreira, António Teixeira, J. A. Rocha, Fernando |
author2_role |
author author author |
dc.contributor.none.fl_str_mv |
Universidade do Minho |
dc.contributor.author.fl_str_mv |
Castro, Filipa Ferreira, António Teixeira, J. A. Rocha, Fernando |
dc.subject.por.fl_str_mv |
Science & Technology |
topic |
Science & Technology |
description |
In the present work, it is reported for the first time the study of the applicability of a novel meso oscillatory flow reactor (meso-OFR) for protein crystallization as a process step. Crystallization assays carried out in the designed device enabled to derive a two-dimensional lysozyme phase diagram (lysozyme concentration against sodium chloride concentration). Results evidence the formation of several types of crystals (different size and shape), with a strong influence of salt concentration on crystal shape. Results also show that lysozyme remains active at the end of the experiments. Furthermore, it was possible to verify the reduction of the metastability zone when compared to lysozyme crystallization conducted under quiescent conditions. Induction times were also measured by online monitoring of the turbidity of the crystallization solution, obtained values being between 41 and 900 minutes. Beyond providing improved understanding of protein phase behavior under oscillatory flow mixing, the results are very promising regarding the feasibility of the designed methodology for protein crystallization as a process step. |
publishDate |
2016 |
dc.date.none.fl_str_mv |
2016 2016-01-01T00:00:00Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/1822/42213 |
url |
http://hdl.handle.net/1822/42213 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Castro, Filipa; Ferreira, António; Teixeira, J. A.; Rocha, Fernando, Protein crystallization as a process step in a novel meso oscillatory flow reactor: study of lysozyme phase behavior. Crystal Growth and Design, 16(7), 3748-3755, 2016 1528-7483 1528-7505 10.1021/acs.cgd.6b00262 http://pubs.acs.org/journal/cgdefu |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
American Chemical Society |
publisher.none.fl_str_mv |
American Chemical Society |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
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Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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RCAAP |
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RCAAP |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository.name.fl_str_mv |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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1799132627557416960 |