RpoB polymorphisms in Mycobacterium tuberculosis complex from a population in Guinea-Bissau

Detalhes bibliográficos
Autor(a) principal: Sutre, AF
Data de Publicação: 2013
Outros Autores: Sanca, A, Mané, A, Henriques, V, Portugal, C, Sancho, L, Cardoso, A, Paixão, E, Queico, CQF, Salem, JI, Antunes, A, Duarte, EL, David, S
Tipo de documento: Artigo de conferência
Idioma: por
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10174/8680
Resumo: The global tuberculosis (TB) threat is diffi cult to evaluate in countries with scarce laboratory facilities where little is known about the genetic basis of drug resistance, including the rpoB RRDR (rifampicin resistance determining region) of the Mycobacterium tuberculosis complex (MTC). The present study aimed at performing a preliminary evaluation of rpoB polymorphisms in a DNA set of MTC isolates from Guinea-Bissau patients. Ninety-four sputum specimens (74 bleach processed and 20 unprocessed) were sent to Lisbon for molecular analysis (n=94) and drug susceptibility testing (n=20). A 369bp region of the rpoB gene (including the 81bp RRDR), was amplifi ed. Sequencing was used as the gold standard for the identifi cation of point mutations. Two polymorphisms were identifi ed: The alteration S531L, present in 3.2% of the specimens, and a new mutation, present in 28.7%, corresponding to nucleotide and amino acid polymorphism C224T and S469L, respectively, detected upstream from the RRDR, and not associated with RMP resistance. An NCBI BLAST revealed M. tuberculosis K85 (M. africanum) as the only strain presenting this polymorphism. The circulation of S531L mutated stains, associated with RMP resistance, points to the urgent need to further investigate RMP resistance in this African region. Moreover, the strains presenting the S469L polymorphism showed no other mutations suggesting that its role on MP´susceptibility/ resistance or in providing a genetic background for the occurrence of RMP resistance associated mutations should be further investigated. Also, future genotyping studies could clarify whether this new mutation is a genetic signature of some M. africanum strains.
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spelling RpoB polymorphisms in Mycobacterium tuberculosis complex from a population in Guinea-BissauMycobacterium tuberculosisRPOBRifampicin resistanceThe global tuberculosis (TB) threat is diffi cult to evaluate in countries with scarce laboratory facilities where little is known about the genetic basis of drug resistance, including the rpoB RRDR (rifampicin resistance determining region) of the Mycobacterium tuberculosis complex (MTC). The present study aimed at performing a preliminary evaluation of rpoB polymorphisms in a DNA set of MTC isolates from Guinea-Bissau patients. Ninety-four sputum specimens (74 bleach processed and 20 unprocessed) were sent to Lisbon for molecular analysis (n=94) and drug susceptibility testing (n=20). A 369bp region of the rpoB gene (including the 81bp RRDR), was amplifi ed. Sequencing was used as the gold standard for the identifi cation of point mutations. Two polymorphisms were identifi ed: The alteration S531L, present in 3.2% of the specimens, and a new mutation, present in 28.7%, corresponding to nucleotide and amino acid polymorphism C224T and S469L, respectively, detected upstream from the RRDR, and not associated with RMP resistance. An NCBI BLAST revealed M. tuberculosis K85 (M. africanum) as the only strain presenting this polymorphism. The circulation of S531L mutated stains, associated with RMP resistance, points to the urgent need to further investigate RMP resistance in this African region. Moreover, the strains presenting the S469L polymorphism showed no other mutations suggesting that its role on MP´susceptibility/ resistance or in providing a genetic background for the occurrence of RMP resistance associated mutations should be further investigated. Also, future genotyping studies could clarify whether this new mutation is a genetic signature of some M. africanum strains.2013-08-20T09:26:55Z2013-08-202013-06-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/conferenceObjecthttp://hdl.handle.net/10174/8680http://hdl.handle.net/10174/8680porSutre AF, Sanca A, Mané A, Henriques V, Portugal C, Sancho L, Cardoso A, Paixão E, Leite CQF, Salem JI, Antunes A, Duarte EL & David. S. 2013. RpoB polymorphisms in Mycobacterium tuberculosis complex from a population in Guinea-Bissau Proceedings of the 34th annual congress of the European Society of Mycobacteriology, June 2013, Florence, Italy p. 84http://vorschau.agentur-konsens.de/ESM/downloads/ESM_Abstract_Book2013_6_web.pdfnaonaosimMVTndndndndndndndndndndndemld@uevora.ptndSutre, AFSanca, AMané, AHenriques, VPortugal, CSancho, LCardoso, APaixão, EQueico, CQFSalem, JIAntunes, ADuarte, ELDavid, Sinfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-01-03T18:49:51Zoai:dspace.uevora.pt:10174/8680Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T01:02:52.298019Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv RpoB polymorphisms in Mycobacterium tuberculosis complex from a population in Guinea-Bissau
title RpoB polymorphisms in Mycobacterium tuberculosis complex from a population in Guinea-Bissau
spellingShingle RpoB polymorphisms in Mycobacterium tuberculosis complex from a population in Guinea-Bissau
Sutre, AF
Mycobacterium tuberculosis
RPOB
Rifampicin resistance
title_short RpoB polymorphisms in Mycobacterium tuberculosis complex from a population in Guinea-Bissau
title_full RpoB polymorphisms in Mycobacterium tuberculosis complex from a population in Guinea-Bissau
title_fullStr RpoB polymorphisms in Mycobacterium tuberculosis complex from a population in Guinea-Bissau
title_full_unstemmed RpoB polymorphisms in Mycobacterium tuberculosis complex from a population in Guinea-Bissau
title_sort RpoB polymorphisms in Mycobacterium tuberculosis complex from a population in Guinea-Bissau
author Sutre, AF
author_facet Sutre, AF
Sanca, A
Mané, A
Henriques, V
Portugal, C
Sancho, L
Cardoso, A
Paixão, E
Queico, CQF
Salem, JI
Antunes, A
Duarte, EL
David, S
author_role author
author2 Sanca, A
Mané, A
Henriques, V
Portugal, C
Sancho, L
Cardoso, A
Paixão, E
Queico, CQF
Salem, JI
Antunes, A
Duarte, EL
David, S
author2_role author
author
author
author
author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Sutre, AF
Sanca, A
Mané, A
Henriques, V
Portugal, C
Sancho, L
Cardoso, A
Paixão, E
Queico, CQF
Salem, JI
Antunes, A
Duarte, EL
David, S
dc.subject.por.fl_str_mv Mycobacterium tuberculosis
RPOB
Rifampicin resistance
topic Mycobacterium tuberculosis
RPOB
Rifampicin resistance
description The global tuberculosis (TB) threat is diffi cult to evaluate in countries with scarce laboratory facilities where little is known about the genetic basis of drug resistance, including the rpoB RRDR (rifampicin resistance determining region) of the Mycobacterium tuberculosis complex (MTC). The present study aimed at performing a preliminary evaluation of rpoB polymorphisms in a DNA set of MTC isolates from Guinea-Bissau patients. Ninety-four sputum specimens (74 bleach processed and 20 unprocessed) were sent to Lisbon for molecular analysis (n=94) and drug susceptibility testing (n=20). A 369bp region of the rpoB gene (including the 81bp RRDR), was amplifi ed. Sequencing was used as the gold standard for the identifi cation of point mutations. Two polymorphisms were identifi ed: The alteration S531L, present in 3.2% of the specimens, and a new mutation, present in 28.7%, corresponding to nucleotide and amino acid polymorphism C224T and S469L, respectively, detected upstream from the RRDR, and not associated with RMP resistance. An NCBI BLAST revealed M. tuberculosis K85 (M. africanum) as the only strain presenting this polymorphism. The circulation of S531L mutated stains, associated with RMP resistance, points to the urgent need to further investigate RMP resistance in this African region. Moreover, the strains presenting the S469L polymorphism showed no other mutations suggesting that its role on MP´susceptibility/ resistance or in providing a genetic background for the occurrence of RMP resistance associated mutations should be further investigated. Also, future genotyping studies could clarify whether this new mutation is a genetic signature of some M. africanum strains.
publishDate 2013
dc.date.none.fl_str_mv 2013-08-20T09:26:55Z
2013-08-20
2013-06-01T00:00:00Z
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dc.identifier.uri.fl_str_mv http://hdl.handle.net/10174/8680
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dc.relation.none.fl_str_mv Sutre AF, Sanca A, Mané A, Henriques V, Portugal C, Sancho L, Cardoso A, Paixão E, Leite CQF, Salem JI, Antunes A, Duarte EL & David. S. 2013. RpoB polymorphisms in Mycobacterium tuberculosis complex from a population in Guinea-Bissau Proceedings of the 34th annual congress of the European Society of Mycobacteriology, June 2013, Florence, Italy p. 84
http://vorschau.agentur-konsens.de/ESM/downloads/ESM_Abstract_Book2013_6_web.pdf
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