Application of response surface methodological approach to optimise Reactive Black 5 decolouration by crude laccase from Trametes pubescens
Autor(a) principal: | |
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Data de Publicação: | 2009 |
Outros Autores: | , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/1822/9489 |
Resumo: | Response surface methodology (RSM) was applied to optimise the decolouration of the diazo dye Reactive Black 5 (RB5) by crude laccase from the white-rot fungus Trametes pubescens. The presence of the redox mediator 1-hydroxybenzotriazole (HBT) greatly improved the decolouration levels of RB5 by crude laccase from T. pubescens. Central composite design (CCD) using RSM with three variables namely redox mediator (HBT), dye (RB5) and enzyme (laccase) concentrations was used in this study to optimise significant correlation between the effects of these variables on the decolouration of RB5. The optimum concentrations of HBT, RB5 and laccase were 1.17 mM, 150 mg/l and 500 U/l, respectively, for a maximum decolouration of RB5 (about 60% in 20 min). A quadratic model was obtained for dye decolouration through this design. The experimental values were in good agreement with the predicted ones and the model was highly significant, the correlation coefficient being 0.965. Interaction between HBT and RB5 concentrations, HBT and laccase concentrations and RB5 and laccase concentrations were negligible. In addition, when the kinetic parameters for RB5 decolouration were calculated according to Hannes–Wolf plot, the following values were obtained: KM of 260.11 mg/l and Vmax 37.59 mg/l min. |
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Application of response surface methodological approach to optimise Reactive Black 5 decolouration by crude laccase from Trametes pubescensLaccaseCentral composite designDecolourationReactive Black 5Response surface methodologyScience & TechnologyResponse surface methodology (RSM) was applied to optimise the decolouration of the diazo dye Reactive Black 5 (RB5) by crude laccase from the white-rot fungus Trametes pubescens. The presence of the redox mediator 1-hydroxybenzotriazole (HBT) greatly improved the decolouration levels of RB5 by crude laccase from T. pubescens. Central composite design (CCD) using RSM with three variables namely redox mediator (HBT), dye (RB5) and enzyme (laccase) concentrations was used in this study to optimise significant correlation between the effects of these variables on the decolouration of RB5. The optimum concentrations of HBT, RB5 and laccase were 1.17 mM, 150 mg/l and 500 U/l, respectively, for a maximum decolouration of RB5 (about 60% in 20 min). A quadratic model was obtained for dye decolouration through this design. The experimental values were in good agreement with the predicted ones and the model was highly significant, the correlation coefficient being 0.965. Interaction between HBT and RB5 concentrations, HBT and laccase concentrations and RB5 and laccase concentrations were negligible. In addition, when the kinetic parameters for RB5 decolouration were calculated according to Hannes–Wolf plot, the following values were obtained: KM of 260.11 mg/l and Vmax 37.59 mg/l min.Erasmus Rovira i Virgili University (Tarragona, Spain)Ramón y Cajal Programme and the European Social FundSpanish Ministry of Education and ScienceElsevierUniversidade do MinhoRoriz, Margarida S.Osma, Johann F.Teixeira, J. A.Rodríguez Couto, S.2009-092009-09-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/1822/9489eng"Journal of Hazardous Materials. ISSN 0304-3894. 169:1-3 (Sept. 2009) 691-696.0304-389410.1016/j.jhazmat.2009.03.15019409701info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-21T12:33:11Zoai:repositorium.sdum.uminho.pt:1822/9489Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T19:28:40.976743Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Application of response surface methodological approach to optimise Reactive Black 5 decolouration by crude laccase from Trametes pubescens |
title |
Application of response surface methodological approach to optimise Reactive Black 5 decolouration by crude laccase from Trametes pubescens |
spellingShingle |
Application of response surface methodological approach to optimise Reactive Black 5 decolouration by crude laccase from Trametes pubescens Roriz, Margarida S. Laccase Central composite design Decolouration Reactive Black 5 Response surface methodology Science & Technology |
title_short |
Application of response surface methodological approach to optimise Reactive Black 5 decolouration by crude laccase from Trametes pubescens |
title_full |
Application of response surface methodological approach to optimise Reactive Black 5 decolouration by crude laccase from Trametes pubescens |
title_fullStr |
Application of response surface methodological approach to optimise Reactive Black 5 decolouration by crude laccase from Trametes pubescens |
title_full_unstemmed |
Application of response surface methodological approach to optimise Reactive Black 5 decolouration by crude laccase from Trametes pubescens |
title_sort |
Application of response surface methodological approach to optimise Reactive Black 5 decolouration by crude laccase from Trametes pubescens |
author |
Roriz, Margarida S. |
author_facet |
Roriz, Margarida S. Osma, Johann F. Teixeira, J. A. Rodríguez Couto, S. |
author_role |
author |
author2 |
Osma, Johann F. Teixeira, J. A. Rodríguez Couto, S. |
author2_role |
author author author |
dc.contributor.none.fl_str_mv |
Universidade do Minho |
dc.contributor.author.fl_str_mv |
Roriz, Margarida S. Osma, Johann F. Teixeira, J. A. Rodríguez Couto, S. |
dc.subject.por.fl_str_mv |
Laccase Central composite design Decolouration Reactive Black 5 Response surface methodology Science & Technology |
topic |
Laccase Central composite design Decolouration Reactive Black 5 Response surface methodology Science & Technology |
description |
Response surface methodology (RSM) was applied to optimise the decolouration of the diazo dye Reactive Black 5 (RB5) by crude laccase from the white-rot fungus Trametes pubescens. The presence of the redox mediator 1-hydroxybenzotriazole (HBT) greatly improved the decolouration levels of RB5 by crude laccase from T. pubescens. Central composite design (CCD) using RSM with three variables namely redox mediator (HBT), dye (RB5) and enzyme (laccase) concentrations was used in this study to optimise significant correlation between the effects of these variables on the decolouration of RB5. The optimum concentrations of HBT, RB5 and laccase were 1.17 mM, 150 mg/l and 500 U/l, respectively, for a maximum decolouration of RB5 (about 60% in 20 min). A quadratic model was obtained for dye decolouration through this design. The experimental values were in good agreement with the predicted ones and the model was highly significant, the correlation coefficient being 0.965. Interaction between HBT and RB5 concentrations, HBT and laccase concentrations and RB5 and laccase concentrations were negligible. In addition, when the kinetic parameters for RB5 decolouration were calculated according to Hannes–Wolf plot, the following values were obtained: KM of 260.11 mg/l and Vmax 37.59 mg/l min. |
publishDate |
2009 |
dc.date.none.fl_str_mv |
2009-09 2009-09-01T00:00:00Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/1822/9489 |
url |
http://hdl.handle.net/1822/9489 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
"Journal of Hazardous Materials. ISSN 0304-3894. 169:1-3 (Sept. 2009) 691-696. 0304-3894 10.1016/j.jhazmat.2009.03.150 19409701 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Elsevier |
publisher.none.fl_str_mv |
Elsevier |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
instname_str |
Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
instacron_str |
RCAAP |
institution |
RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
collection |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository.name.fl_str_mv |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
repository.mail.fl_str_mv |
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1799132783306604544 |