Development and application of a novel peptide nucleic acid Probe for the specific detection of cronobacter genomospecies (Enterobacter sakazakii) in powdered infant formula

Detalhes bibliográficos
Autor(a) principal: Almeida, Carina
Data de Publicação: 2009
Outros Autores: Azevedo, N. F., Iversen, C., Fanning, S., Keevil, C. W., Vieira, M. J.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/1822/9454
Resumo: Here, we report a fluorescence in situ hybridization (FISH) method for rapid detection of Cronobacter strains in powdered infant formula (PIF) using a novel peptide nucleic acid (PNA) probe. Laboratory tests with several Enterobacteriaceae species showed that the specificity and sensitivity of the method were 100%. FISH using PNA could detect as few as 1 CFU per 10 g of Cronobacter in PIF after an 8-h enrichment step, even in a mixed population containing bacterial contaminants.
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spelling Development and application of a novel peptide nucleic acid Probe for the specific detection of cronobacter genomospecies (Enterobacter sakazakii) in powdered infant formulaScience & TechnologyHere, we report a fluorescence in situ hybridization (FISH) method for rapid detection of Cronobacter strains in powdered infant formula (PIF) using a novel peptide nucleic acid (PNA) probe. Laboratory tests with several Enterobacteriaceae species showed that the specificity and sensitivity of the method were 100%. FISH using PNA could detect as few as 1 CFU per 10 g of Cronobacter in PIF after an 8-h enrichment step, even in a mixed population containing bacterial contaminants.Fundação para a Ciência e a Tecnologia (FCT)American Society for Microbiology (ASM)Universidade do MinhoAlmeida, CarinaAzevedo, N. F.Iversen, C.Fanning, S.Keevil, C. W.Vieira, M. J.2009-052009-05-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/1822/9454eng"Applied and Environmental Microbiology". ISSN 0099-2240. 75:9 (May 2009) 2925-2930.0099-224010.1128/AEM.02470-0819270117info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-21T12:24:50Zoai:repositorium.sdum.uminho.pt:1822/9454Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T19:18:56.170153Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Development and application of a novel peptide nucleic acid Probe for the specific detection of cronobacter genomospecies (Enterobacter sakazakii) in powdered infant formula
title Development and application of a novel peptide nucleic acid Probe for the specific detection of cronobacter genomospecies (Enterobacter sakazakii) in powdered infant formula
spellingShingle Development and application of a novel peptide nucleic acid Probe for the specific detection of cronobacter genomospecies (Enterobacter sakazakii) in powdered infant formula
Almeida, Carina
Science & Technology
title_short Development and application of a novel peptide nucleic acid Probe for the specific detection of cronobacter genomospecies (Enterobacter sakazakii) in powdered infant formula
title_full Development and application of a novel peptide nucleic acid Probe for the specific detection of cronobacter genomospecies (Enterobacter sakazakii) in powdered infant formula
title_fullStr Development and application of a novel peptide nucleic acid Probe for the specific detection of cronobacter genomospecies (Enterobacter sakazakii) in powdered infant formula
title_full_unstemmed Development and application of a novel peptide nucleic acid Probe for the specific detection of cronobacter genomospecies (Enterobacter sakazakii) in powdered infant formula
title_sort Development and application of a novel peptide nucleic acid Probe for the specific detection of cronobacter genomospecies (Enterobacter sakazakii) in powdered infant formula
author Almeida, Carina
author_facet Almeida, Carina
Azevedo, N. F.
Iversen, C.
Fanning, S.
Keevil, C. W.
Vieira, M. J.
author_role author
author2 Azevedo, N. F.
Iversen, C.
Fanning, S.
Keevil, C. W.
Vieira, M. J.
author2_role author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade do Minho
dc.contributor.author.fl_str_mv Almeida, Carina
Azevedo, N. F.
Iversen, C.
Fanning, S.
Keevil, C. W.
Vieira, M. J.
dc.subject.por.fl_str_mv Science & Technology
topic Science & Technology
description Here, we report a fluorescence in situ hybridization (FISH) method for rapid detection of Cronobacter strains in powdered infant formula (PIF) using a novel peptide nucleic acid (PNA) probe. Laboratory tests with several Enterobacteriaceae species showed that the specificity and sensitivity of the method were 100%. FISH using PNA could detect as few as 1 CFU per 10 g of Cronobacter in PIF after an 8-h enrichment step, even in a mixed population containing bacterial contaminants.
publishDate 2009
dc.date.none.fl_str_mv 2009-05
2009-05-01T00:00:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/1822/9454
url http://hdl.handle.net/1822/9454
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv "Applied and Environmental Microbiology". ISSN 0099-2240. 75:9 (May 2009) 2925-2930.
0099-2240
10.1128/AEM.02470-08
19270117
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv American Society for Microbiology (ASM)
publisher.none.fl_str_mv American Society for Microbiology (ASM)
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
repository.mail.fl_str_mv
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