Surface properties of Yarrowia lipolytica and their relevance to γ-decalactone formation from methyl ricinoleate

• Autor(a) principal: Aguedo, Mário
• Data de Publicação: 2005
• Outros Autores: Waché, Yves, Belin, Jean-Marc, Teixeira, J. A.
• Tipo de documento: Artigo
• Idioma: eng
• Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
• Texto Completo: http://hdl.handle.net/1822/2566
• topic: Biotransformation; Contact angles; Surface properties; Yarrowia lipolytica; Zeta potential; Science & Technology

The surface of the lipid-degrading yeast, Yarrowia lipolytica, was characterized by contact angle and zeta potential (ζ) measurements. The cells were mainly hydrophilic with a negative charge that was only affected from pH 2 to 4. To study the effects of the surface charges on the biotransformation of methyl ricinoleate into the aroma compound, γ-decalactone, the ζ values of the substrate droplets were modified by adding a cationic surfactant into the medium at concentrations that did not diminish cell viability: the adhesion of the lipid substrate to the cells was increased but not the overall performance of the process, therefore the adhesion is not the rate limiting here. Our methodology offers interesting perspectives for further applications.