Peptidylprolyl isomerase C (Ppic) regulates invariant Natural Killer T cell (iNKT) differentiation in mice

Detalhes bibliográficos
Autor(a) principal: Paiva, Ricardo S.
Data de Publicação: 2021
Outros Autores: Ramos, Camila V., Azenha, Sara R., Alves, Carolina, Basto, Afonso P., Graça, Luís, Martins, Vera C.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10400.5/22540
Resumo: Research Areas: Immunology
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spelling Peptidylprolyl isomerase C (Ppic) regulates invariant Natural Killer T cell (iNKT) differentiation in micePeptidylprolyl isomerase CCyclophilin CHematopoiesisPpicT-cell developmentThymopoiesisResearch Areas: ImmunologyPeptidyl-prolyl cis-trans isomerase C (Ppic) is expressed in several bone marrow (BM) hematopoietic progenitors and in T-cell precursors. Since the expression profile of Ppic in the hematoimmune system was suggestive that it could play a role in hematopoiesis and/or T lymphocyte differentiation, we sought to test that hypothesis in vivo. Specifically, we generated a Ppic-deficient mouse model by targeting the endogenous locus by CRISPR/Cas9 and tested the requirement of Ppic in hematopoiesis. Several immune cell lineages covering BM progenitors, lymphocyte precursors, as well as mature cells at the periphery were analyzed. While most lineages were unaffected, invariant NKT (iNKT) cells were reduced in percentage and absolute cell numbers in the Ppic-deficient thymus. This affected the most mature stages in the thymus, S2 and S3, and the phenotype was maintained at the periphery. Additionally, immature transitional T1 and T2 B lymphocytes were increased in the Ppic-deficient spleen, but the phenotype was lost in mature B lymphocytes. In sum, our data show that Ppic is dispensable for myeloid cells, platelets, erythrocytes, αβ, and γδ T lymphocytes in vivo in the steady state, while being involved in B- and iNKT cell differentiation.WileyRepositório da Universidade de LisboaPaiva, Ricardo S.Ramos, Camila V.Azenha, Sara R.Alves, CarolinaBasto, Afonso P.Graça, LuísMartins, Vera C.2021-11-10T23:10:32Z2021-082021-08-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10400.5/22540engPaiva RS, Ramos CV, Azenha SR, Alves C, Basto AP, Graça L, Martins VC. 2021. Peptidylprolyl isomerase C (Ppic) regulates invariant Natural Killer T cell (iNKT) differentiation in mice. European Journal of Immunology, 51(8):1968–1979. DOI:10.1002/eji.2020489240014-298010.1002/eji.2020489241521-4141info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-03-06T14:52:04Zoai:www.repository.utl.pt:10400.5/22540Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T17:06:56.657948Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Peptidylprolyl isomerase C (Ppic) regulates invariant Natural Killer T cell (iNKT) differentiation in mice
title Peptidylprolyl isomerase C (Ppic) regulates invariant Natural Killer T cell (iNKT) differentiation in mice
spellingShingle Peptidylprolyl isomerase C (Ppic) regulates invariant Natural Killer T cell (iNKT) differentiation in mice
Paiva, Ricardo S.
Peptidylprolyl isomerase C
Cyclophilin C
Hematopoiesis
Ppic
T-cell development
Thymopoiesis
title_short Peptidylprolyl isomerase C (Ppic) regulates invariant Natural Killer T cell (iNKT) differentiation in mice
title_full Peptidylprolyl isomerase C (Ppic) regulates invariant Natural Killer T cell (iNKT) differentiation in mice
title_fullStr Peptidylprolyl isomerase C (Ppic) regulates invariant Natural Killer T cell (iNKT) differentiation in mice
title_full_unstemmed Peptidylprolyl isomerase C (Ppic) regulates invariant Natural Killer T cell (iNKT) differentiation in mice
title_sort Peptidylprolyl isomerase C (Ppic) regulates invariant Natural Killer T cell (iNKT) differentiation in mice
author Paiva, Ricardo S.
author_facet Paiva, Ricardo S.
Ramos, Camila V.
Azenha, Sara R.
Alves, Carolina
Basto, Afonso P.
Graça, Luís
Martins, Vera C.
author_role author
author2 Ramos, Camila V.
Azenha, Sara R.
Alves, Carolina
Basto, Afonso P.
Graça, Luís
Martins, Vera C.
author2_role author
author
author
author
author
author
dc.contributor.none.fl_str_mv Repositório da Universidade de Lisboa
dc.contributor.author.fl_str_mv Paiva, Ricardo S.
Ramos, Camila V.
Azenha, Sara R.
Alves, Carolina
Basto, Afonso P.
Graça, Luís
Martins, Vera C.
dc.subject.por.fl_str_mv Peptidylprolyl isomerase C
Cyclophilin C
Hematopoiesis
Ppic
T-cell development
Thymopoiesis
topic Peptidylprolyl isomerase C
Cyclophilin C
Hematopoiesis
Ppic
T-cell development
Thymopoiesis
description Research Areas: Immunology
publishDate 2021
dc.date.none.fl_str_mv 2021-11-10T23:10:32Z
2021-08
2021-08-01T00:00:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10400.5/22540
url http://hdl.handle.net/10400.5/22540
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Paiva RS, Ramos CV, Azenha SR, Alves C, Basto AP, Graça L, Martins VC. 2021. Peptidylprolyl isomerase C (Ppic) regulates invariant Natural Killer T cell (iNKT) differentiation in mice. European Journal of Immunology, 51(8):1968–1979. DOI:10.1002/eji.202048924
0014-2980
10.1002/eji.202048924
1521-4141
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Wiley
publisher.none.fl_str_mv Wiley
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
repository.mail.fl_str_mv
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