Fungal community in chestnut orchards with different Hypholoma fasciculare aboveground abundance: potential implications for sustainable production
Autor(a) principal: | |
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Data de Publicação: | 2019 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | https://doi.org/10.19084/RCA15153 |
Resumo: | European chestnut (Castanea sativa Mill.) trees have a significant impact in the Portuguese economy, due to the production of chestnuts and wood, and other related activities, such as mushroom collection and hunt. Hypholoma fasciculare (Huds.) is a saprophytic fungus widely distributed in Trás-os-Montes (northeast of Portugal) chestnut groves that displays an in vitro strong antagonistic activity against ectomycorrhizal (EMC) fungi. In this study, the above- and belowground fungal diversity was evaluated in three chestnut orchards, containing distinct H. fasciculare sporocarps abundances in an attempt to better understand the potential of this fungus to attain an improved chestnut tree sustainable productivity. Aboveground analysis was performed based on macrofungi collection during fruiting seasons (spring and autumn) of two consecutive years. Belowground evaluation was based on the metabarcoding of chestnut orchards soil DNA, using the fungal barcode ITS1 and a high-throughput sequencing (454-sequencing) approach. Although all collected fruitbodies were identified as being produced by Basidiomycota fungi, a more diversified fungal community was revealed by the belowground approach. Both approaches have revealed a rich and abundant ECM community in all chestnut orchards. The correlation between the abundance of H. fasciculare fruitbodies and specific fungal guilds fruitbodies/reads suggests that this fungus may affect soil fungal community, mainly ECM and phytoparasites, as well as species composition of fungal communities. Although not conclusive, the results suggest that H. fasciculare presence could be critical for sustainable chestnut ecosystems. |
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Fungal community in chestnut orchards with different Hypholoma fasciculare aboveground abundance: potential implications for sustainable productionComunidade fúngica de soutos com diferentes abundâncias de Hypholoma fasciculare: potenciais implicações para a produção sustentável de castanheiroGeralEuropean chestnut (Castanea sativa Mill.) trees have a significant impact in the Portuguese economy, due to the production of chestnuts and wood, and other related activities, such as mushroom collection and hunt. Hypholoma fasciculare (Huds.) is a saprophytic fungus widely distributed in Trás-os-Montes (northeast of Portugal) chestnut groves that displays an in vitro strong antagonistic activity against ectomycorrhizal (EMC) fungi. In this study, the above- and belowground fungal diversity was evaluated in three chestnut orchards, containing distinct H. fasciculare sporocarps abundances in an attempt to better understand the potential of this fungus to attain an improved chestnut tree sustainable productivity. Aboveground analysis was performed based on macrofungi collection during fruiting seasons (spring and autumn) of two consecutive years. Belowground evaluation was based on the metabarcoding of chestnut orchards soil DNA, using the fungal barcode ITS1 and a high-throughput sequencing (454-sequencing) approach. Although all collected fruitbodies were identified as being produced by Basidiomycota fungi, a more diversified fungal community was revealed by the belowground approach. Both approaches have revealed a rich and abundant ECM community in all chestnut orchards. The correlation between the abundance of H. fasciculare fruitbodies and specific fungal guilds fruitbodies/reads suggests that this fungus may affect soil fungal community, mainly ECM and phytoparasites, as well as species composition of fungal communities. Although not conclusive, the results suggest that H. fasciculare presence could be critical for sustainable chestnut ecosystems.O castanheiro europeu (Castanea sativa Mill.) tem um enorme impacto na economia Portuguesa, sobretudo devido à produção de castanha e de madeira, mas também de actividades relacionadas com a colheira de cogumelos e a caça. Hypholoma fasciculare (Huds.) é um fungo saprófita amplamente distribuído nos soutos da região de Trás-os-Montes (nordeste de Portugal) e que apresenta, em condições in vitro, uma forte actividade antagonista contra fungos ectomicorrízicos. Neste estudo pretendeu-se avaliar a comunidade fúngica presente na superfície e no sub-solo de soutos contendo diferentes abundâncias de carpóforos de H. fasciculare, numa tentativa de compreender melhor a possibilidade deste fungo melhorar a produção sustentável do castanheiro. A diversidade fúngica à superfície do solo foi avaliada recorrendo à colheita de cogumelos durante o outono e a primavera, ao longo de dois anos consecutivos. A diversidade no sub-solo foi avaliada molecularmente pela extração do DNA do solo de soutos, amplificação da região ITS1 e sequenciação de nova geração (sequenciação 454). Apesar dos cogumelos colhidos serem todos produzidos por fungos Basidiomicetes, uma comunidade fúngica mais diversificada foi revelada pela abordagem molecular. No entanto, ambas as abordagens revelaram uma comunidade rica e abundante de fungos ECM em todos os soutos. A correlação efetuada entre a abundância de cogumelos de H. fasciculare e os grupos tróficos dos fungos presentes, avaliada por cogumelos/sequências, sugere que este fungo pode afetar a comunidade fúngica do solo, principalmente os fungos ectomicorrízicos e fitoparasitas, assim como a composição em espécies da comunidade. Apesar de não serem conclusivos, os resultados sugerem que a presença de H. fasciculare poderá ser crítica para a sustentabilidade de ecossistemas de castanheiro.Sociedade de Ciências Agrárias de Portugal2019-01-09T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttps://doi.org/10.19084/RCA15153eng2183-041X0871-018XReis, FranciscaPereira, EricTavares, RuiBaptista, PaulaLino-Neto, Teresainfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2022-09-06T09:24:29Zoai:ojs.revistas.rcaap.pt:article/16435Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T15:30:52.748713Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Fungal community in chestnut orchards with different Hypholoma fasciculare aboveground abundance: potential implications for sustainable production Comunidade fúngica de soutos com diferentes abundâncias de Hypholoma fasciculare: potenciais implicações para a produção sustentável de castanheiro |
title |
Fungal community in chestnut orchards with different Hypholoma fasciculare aboveground abundance: potential implications for sustainable production |
spellingShingle |
Fungal community in chestnut orchards with different Hypholoma fasciculare aboveground abundance: potential implications for sustainable production Reis, Francisca Geral |
title_short |
Fungal community in chestnut orchards with different Hypholoma fasciculare aboveground abundance: potential implications for sustainable production |
title_full |
Fungal community in chestnut orchards with different Hypholoma fasciculare aboveground abundance: potential implications for sustainable production |
title_fullStr |
Fungal community in chestnut orchards with different Hypholoma fasciculare aboveground abundance: potential implications for sustainable production |
title_full_unstemmed |
Fungal community in chestnut orchards with different Hypholoma fasciculare aboveground abundance: potential implications for sustainable production |
title_sort |
Fungal community in chestnut orchards with different Hypholoma fasciculare aboveground abundance: potential implications for sustainable production |
author |
Reis, Francisca |
author_facet |
Reis, Francisca Pereira, Eric Tavares, Rui Baptista, Paula Lino-Neto, Teresa |
author_role |
author |
author2 |
Pereira, Eric Tavares, Rui Baptista, Paula Lino-Neto, Teresa |
author2_role |
author author author author |
dc.contributor.author.fl_str_mv |
Reis, Francisca Pereira, Eric Tavares, Rui Baptista, Paula Lino-Neto, Teresa |
dc.subject.por.fl_str_mv |
Geral |
topic |
Geral |
description |
European chestnut (Castanea sativa Mill.) trees have a significant impact in the Portuguese economy, due to the production of chestnuts and wood, and other related activities, such as mushroom collection and hunt. Hypholoma fasciculare (Huds.) is a saprophytic fungus widely distributed in Trás-os-Montes (northeast of Portugal) chestnut groves that displays an in vitro strong antagonistic activity against ectomycorrhizal (EMC) fungi. In this study, the above- and belowground fungal diversity was evaluated in three chestnut orchards, containing distinct H. fasciculare sporocarps abundances in an attempt to better understand the potential of this fungus to attain an improved chestnut tree sustainable productivity. Aboveground analysis was performed based on macrofungi collection during fruiting seasons (spring and autumn) of two consecutive years. Belowground evaluation was based on the metabarcoding of chestnut orchards soil DNA, using the fungal barcode ITS1 and a high-throughput sequencing (454-sequencing) approach. Although all collected fruitbodies were identified as being produced by Basidiomycota fungi, a more diversified fungal community was revealed by the belowground approach. Both approaches have revealed a rich and abundant ECM community in all chestnut orchards. The correlation between the abundance of H. fasciculare fruitbodies and specific fungal guilds fruitbodies/reads suggests that this fungus may affect soil fungal community, mainly ECM and phytoparasites, as well as species composition of fungal communities. Although not conclusive, the results suggest that H. fasciculare presence could be critical for sustainable chestnut ecosystems. |
publishDate |
2019 |
dc.date.none.fl_str_mv |
2019-01-09T00:00:00Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://doi.org/10.19084/RCA15153 |
url |
https://doi.org/10.19084/RCA15153 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
2183-041X 0871-018X |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.publisher.none.fl_str_mv |
Sociedade de Ciências Agrárias de Portugal |
publisher.none.fl_str_mv |
Sociedade de Ciências Agrárias de Portugal |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
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Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
instacron_str |
RCAAP |
institution |
RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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1799130175230705664 |