Cytochrome c nitrite reductase from desulfovibrio desulfuricans ATCC 27774
Autor(a) principal: | |
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Data de Publicação: | 2003 |
Outros Autores: | , , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10362/1658 |
Resumo: | The Journal of Biological Chemistry Vol. 278, No. 19, Issue of May 9, pp. 17455–17465, 2003 |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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7160 |
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Cytochrome c nitrite reductase from desulfovibrio desulfuricans ATCC 27774The relevance of the two calcium sites inthe structure of the catalytic subunit (NrfA)The Journal of Biological Chemistry Vol. 278, No. 19, Issue of May 9, pp. 17455–17465, 2003The gene encoding cytochrome c nitrite reductase(NrfA) from Desulfovibrio desulfuricans ATCC 27774 was sequenced and the crystal structure of the enzyme was determined to 2.3-Å resolution. In comparison with homologous structures, it presents structural differences mainly located at the regions surrounding the putative substrate inlet and product outlet, and includes a well defined second calcium site with octahedral geometry, coordinated to propionates of hemes 3 and 4, and caged by a loop non-existent in the previous structures. The highly negative electrostatic potential in the environment around hemes 3 and 4 suggests that the main role of this calcium ion may not be electrostatic but structural, namely in the stabilization of the conformation of the additional loop that cages it and influences the solvent accessibility of heme 4. The NrfA active site is similar to that of peroxidases with a nearby calcium site at the heme distal side nearly in the same location as occurs in the class II and class III peroxidases. This fact suggests that the calcium ion at the distal side of the active site in the NrfA enzymes may have a similar physiological role to that reported for the peroxidases.ASBMBRUNMoura, IsabelCunha, Carlos A.Macieira, SofiaDias, João M.Almeida, Gabriela Machado deGonçalves, Luisa L.Costa, CristinaLampreia, JorgeHuber, RobertMoura, José J. G.Romão, Maria J.2008-09-23T14:33:20Z20032003-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10362/1658enginfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-03-11T03:31:38Zoai:run.unl.pt:10362/1658Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T03:14:47.005912Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Cytochrome c nitrite reductase from desulfovibrio desulfuricans ATCC 27774 The relevance of the two calcium sites inthe structure of the catalytic subunit (NrfA) |
title |
Cytochrome c nitrite reductase from desulfovibrio desulfuricans ATCC 27774 |
spellingShingle |
Cytochrome c nitrite reductase from desulfovibrio desulfuricans ATCC 27774 Moura, Isabel |
title_short |
Cytochrome c nitrite reductase from desulfovibrio desulfuricans ATCC 27774 |
title_full |
Cytochrome c nitrite reductase from desulfovibrio desulfuricans ATCC 27774 |
title_fullStr |
Cytochrome c nitrite reductase from desulfovibrio desulfuricans ATCC 27774 |
title_full_unstemmed |
Cytochrome c nitrite reductase from desulfovibrio desulfuricans ATCC 27774 |
title_sort |
Cytochrome c nitrite reductase from desulfovibrio desulfuricans ATCC 27774 |
author |
Moura, Isabel |
author_facet |
Moura, Isabel Cunha, Carlos A. Macieira, Sofia Dias, João M. Almeida, Gabriela Machado de Gonçalves, Luisa L. Costa, Cristina Lampreia, Jorge Huber, Robert Moura, José J. G. Romão, Maria J. |
author_role |
author |
author2 |
Cunha, Carlos A. Macieira, Sofia Dias, João M. Almeida, Gabriela Machado de Gonçalves, Luisa L. Costa, Cristina Lampreia, Jorge Huber, Robert Moura, José J. G. Romão, Maria J. |
author2_role |
author author author author author author author author author author |
dc.contributor.none.fl_str_mv |
RUN |
dc.contributor.author.fl_str_mv |
Moura, Isabel Cunha, Carlos A. Macieira, Sofia Dias, João M. Almeida, Gabriela Machado de Gonçalves, Luisa L. Costa, Cristina Lampreia, Jorge Huber, Robert Moura, José J. G. Romão, Maria J. |
description |
The Journal of Biological Chemistry Vol. 278, No. 19, Issue of May 9, pp. 17455–17465, 2003 |
publishDate |
2003 |
dc.date.none.fl_str_mv |
2003 2003-01-01T00:00:00Z 2008-09-23T14:33:20Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10362/1658 |
url |
http://hdl.handle.net/10362/1658 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
ASBMB |
publisher.none.fl_str_mv |
ASBMB |
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reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
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Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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RCAAP |
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RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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