A role of supraspinal galanin in behavioural hyperalgesia in the rat

Detalhes bibliográficos
Autor(a) principal: Amorim, Diana
Data de Publicação: 2014
Outros Autores: Pereira, Ana David, Marques, Patrícia, Puga, Sónia Andreia Silva, Rebelo, Patrícia, Costa, Patrício Soares, Pertovaara, Antti, Almeida, Armando, Ribeiro, Filipa Pinto
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/1822/32480
Resumo: Data from the manuscript: "A role of supraspinal galanin in behavioural hyperalgesia in the rat" – by Amorim et al, PLoS One. Available at: http://hdl.handle.net/1822/30584
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spelling A role of supraspinal galanin in behavioural hyperalgesia in the ratScience & TechnologyData from the manuscript: "A role of supraspinal galanin in behavioural hyperalgesia in the rat" – by Amorim et al, PLoS One. Available at: http://hdl.handle.net/1822/30584INTRODUCTION: In chronic pain disorders, galanin (GAL) is able to either facilitate or inhibit nociception in the spinal cord but the contribution of supraspinal galanin to pain signalling is mostly unknown. The dorsomedial nucleus of the hypothalamus (DMH) is rich in galanin receptors (GALR) and is involved in behavioural hyperalgesia. In this study, we evaluated the contribution of supraspinal GAL to behavioural hyperalgesia in experimental monoarthritis. METHODS: In Wistar-Han males with a four week kaolin/carrageenan-induced monoarthritis (ARTH), paw-withdrawal latency (PWL) was assessed before and after DMH administration of exogenous GAL, a non-specific GALR antagonist (M40), a specific GALR1 agonist (M617) and a specific GALR2 antagonist (M871). Additionally, the analysis of c-Fos expression after GAL injection in the DMH was used to investigate the potential involvement of brainstem pain control centres. Finally, electrophysiological recordings were performed to evaluate whether pronociceptive On- or antinociceptive Off-like cells in the rostral ventromedial medulla (RVM) relay the effect of GAL. RESULTS: Exogenous GAL in the DMH decreased PWL in ARTH and SHAM animals, an effect that was mimicked by a GALR1 agonist (M617). In SHAM animals, an unselective GALR antagonist (M40) increased PWL, while a GALR2 antagonist (M871) decreased PWL. M40 or M871 failed to influence PWL in ARTH animals. Exogenous GAL increased c-Fos expression in the RVM and dorsal raphe nucleus (DRN), with effects being more prominent in SHAM than ARTH animals. Exogenous GAL failed to influence activity of RVM On- or Off-like cells of SHAM and ARTH animals. CONCLUSIONS: Overall, exogenous GAL in the DMH had a pronociceptive effect that is mediated by GALR1 in healthy and arthritic animals and is associated with alterations of c-Fos expression in RVM and DRN that are serotonergic brainstem nuclei known to be involved in the regulation of pain.This study was supported by grants from the Portuguese Science Foundation (FCT) Project nu PTDC/SAU-NEU/108557/2008, FEDER-COMPETE, by the Academy of Finland and the Sigrid Jusélius Foundation, Helsinki, Finland. DA was supported by FCT grant SFRH/BD/71219/2010 and ADP was supported by FCT grant SFRH/BD/90374/2012. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.Public Library of ScienceUniversidade do MinhoAmorim, DianaPereira, Ana DavidMarques, PatríciaPuga, Sónia Andreia SilvaRebelo, PatríciaCosta, Patrício SoaresPertovaara, AnttiAlmeida, ArmandoRibeiro, Filipa Pinto20142014-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/1822/32480eng1932-620310.1371/journal.pone.011307725405608http://www.plosone.org/info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-21T11:57:28Zoai:repositorium.sdum.uminho.pt:1822/32480Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T18:47:07.774195Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv A role of supraspinal galanin in behavioural hyperalgesia in the rat
title A role of supraspinal galanin in behavioural hyperalgesia in the rat
spellingShingle A role of supraspinal galanin in behavioural hyperalgesia in the rat
Amorim, Diana
Science & Technology
title_short A role of supraspinal galanin in behavioural hyperalgesia in the rat
title_full A role of supraspinal galanin in behavioural hyperalgesia in the rat
title_fullStr A role of supraspinal galanin in behavioural hyperalgesia in the rat
title_full_unstemmed A role of supraspinal galanin in behavioural hyperalgesia in the rat
title_sort A role of supraspinal galanin in behavioural hyperalgesia in the rat
author Amorim, Diana
author_facet Amorim, Diana
Pereira, Ana David
Marques, Patrícia
Puga, Sónia Andreia Silva
Rebelo, Patrícia
Costa, Patrício Soares
Pertovaara, Antti
Almeida, Armando
Ribeiro, Filipa Pinto
author_role author
author2 Pereira, Ana David
Marques, Patrícia
Puga, Sónia Andreia Silva
Rebelo, Patrícia
Costa, Patrício Soares
Pertovaara, Antti
Almeida, Armando
Ribeiro, Filipa Pinto
author2_role author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade do Minho
dc.contributor.author.fl_str_mv Amorim, Diana
Pereira, Ana David
Marques, Patrícia
Puga, Sónia Andreia Silva
Rebelo, Patrícia
Costa, Patrício Soares
Pertovaara, Antti
Almeida, Armando
Ribeiro, Filipa Pinto
dc.subject.por.fl_str_mv Science & Technology
topic Science & Technology
description Data from the manuscript: "A role of supraspinal galanin in behavioural hyperalgesia in the rat" – by Amorim et al, PLoS One. Available at: http://hdl.handle.net/1822/30584
publishDate 2014
dc.date.none.fl_str_mv 2014
2014-01-01T00:00:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/1822/32480
url http://hdl.handle.net/1822/32480
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 1932-6203
10.1371/journal.pone.0113077
25405608
http://www.plosone.org/
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Public Library of Science
publisher.none.fl_str_mv Public Library of Science
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
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