Thyroid axis disruption by goitrogens: a molecular and functional approach

Detalhes bibliográficos
Autor(a) principal: Guerreiro, Eduarda Mazagão
Data de Publicação: 2008
Tipo de documento: Dissertação
Idioma: por
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10400.1/558
Resumo: Dissertação mest., Engenharia Biológica, Universidade do Algarve, 2008
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spelling Thyroid axis disruption by goitrogens: a molecular and functional approachTesesEngenharia biológicaPoluição ambientalZebra (Peixes)Dissertação mest., Engenharia Biológica, Universidade do Algarve, 2008The number of chemicals to which organisms are exposed as a consequence of environmental contamination by Industry and Agriculture is increasing. However, there is a severe lack of information about the biological effects of many frequently occurring chemicals. The present thesis is focused on the effects on the thyroid tissue of adult zebra fish of the antimicrobial agent Triclosan (TCS) which is frequently found in personnel hygiene products and foodstuff. After 21 days exposure of adult zebrafish to TCS (100 mg/kg/day) and the thyroid disruptor, Propylthiouracil (PTU, positive control) (5 mg/kg/day), histological observation of thyroid tissue in sections of the pharynx was performed. These observations indicated significant (p<0.05) increase in follicle areas coupled with the decrease of thyrocyte height (thyrocyte inactivation) in TCS treated zebrafish. In the PTU treated zebrafish only a reduction in thyrocyte activity was observed. In order to understand how the test chemical TCS might bring about its effect, the gene expression of Thyroglobulin (Tg), Thyroid Peroxidase (TPO), Sodium-Iodine Symporter (NIS) and Cathepsin B (CtsB) together with Thyroid Stimulating Hormone (TSH) in whole zebrafish head was determined using quantitative real-time Polymerase Chain Reaction (qRT-PCR). After sample normalization against the zebrafish spermatogenic glyceraldehyde-3-phosphate dehydrogenases (GAPDH-2), validated as reference gene for this study, a significant increase (p<0.05) in expression of TSH and NIS were detected. The expression of pituitary TSH increased in the PTU and TCS groups compared to the control group, while NIS expression only increased in the TCS treated zebrafish. For the remaining genes, no significant changes in gene expression were detected, maybe as a consequence of high individual variation. In summary, the results of the study indicate that the drug TCS at a concentration of 100 mg/kg/day for 21 days appears to influence TH synthesis. The increase in TSH and NIS transcription coupled to the inactivation of the thyroid tissue observed in this work, are indicative of effects caused by a reduction in circulating THs. It remains to be established the mechanism by which TCS reduces thyroid tissue activity in adult zebra fish.Power, DeborahPinto, PatriciaSapientiaGuerreiro, Eduarda Mazagão2011-09-07T16:05:00Z20082008-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesisapplication/pdfapplication/pdfhttp://hdl.handle.net/10400.1/558por574.64 GUE*Thy Caveinfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-24T10:11:20Zoai:sapientia.ualg.pt:10400.1/558Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T19:54:54.395808Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Thyroid axis disruption by goitrogens: a molecular and functional approach
title Thyroid axis disruption by goitrogens: a molecular and functional approach
spellingShingle Thyroid axis disruption by goitrogens: a molecular and functional approach
Guerreiro, Eduarda Mazagão
Teses
Engenharia biológica
Poluição ambiental
Zebra (Peixes)
title_short Thyroid axis disruption by goitrogens: a molecular and functional approach
title_full Thyroid axis disruption by goitrogens: a molecular and functional approach
title_fullStr Thyroid axis disruption by goitrogens: a molecular and functional approach
title_full_unstemmed Thyroid axis disruption by goitrogens: a molecular and functional approach
title_sort Thyroid axis disruption by goitrogens: a molecular and functional approach
author Guerreiro, Eduarda Mazagão
author_facet Guerreiro, Eduarda Mazagão
author_role author
dc.contributor.none.fl_str_mv Power, Deborah
Pinto, Patricia
Sapientia
dc.contributor.author.fl_str_mv Guerreiro, Eduarda Mazagão
dc.subject.por.fl_str_mv Teses
Engenharia biológica
Poluição ambiental
Zebra (Peixes)
topic Teses
Engenharia biológica
Poluição ambiental
Zebra (Peixes)
description Dissertação mest., Engenharia Biológica, Universidade do Algarve, 2008
publishDate 2008
dc.date.none.fl_str_mv 2008
2008-01-01T00:00:00Z
2011-09-07T16:05:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/masterThesis
format masterThesis
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10400.1/558
url http://hdl.handle.net/10400.1/558
dc.language.iso.fl_str_mv por
language por
dc.relation.none.fl_str_mv 574.64 GUE*Thy Cave
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
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repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
repository.mail.fl_str_mv
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