A simple microfluidic device for the deformability assessment of blood cells in a continuous flow

Detalhes bibliográficos
Autor(a) principal: Rodrigues, Raquel Oliveira
Data de Publicação: 2015
Outros Autores: Pinho, Diana, Faustino, Vera, Lima, Rui A.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10198/16100
Resumo: Blood flow presents several interesting phenomena in microcirculation that can be used to develop microfluidic devices capable to promote blood cells separation and analysis in continuous flow. In the last decade there have been numerous microfluidic studies focused on the deformation of red blood cells (RBCs) flowing through geometries mimicking microvessels. In contrast, studies focusing on the deformation of white blood cells (WBCs) are scarce despite this phenomenon often happens in the microcirculation. In this work, we present a novel integrative microfluidic device able to perform continuous separation of a desired amount of blood cells, without clogging or jamming, and at the same time, capable to assess the deformation index (DI) of both WBCs and RBCs. To determine the DI of both WBCs and RBCs, a hyperbolic converging microchannel was used, as well as a suitable image analysis technique to measure the DIs of these blood cells along the regions of interest. The results show that the WBCs have a much lower deformability than RBCs when subjected to the same in vitro flow conditions, which is directly related to their cytoskeleton and nucleus contents. The proposed strategy can be easily transformed into a simple and inexpensive diagnostic microfluidic system to simultaneously separate and assess blood cells deformability.
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spelling A simple microfluidic device for the deformability assessment of blood cells in a continuous flowBlood on chipsCell separation and deformabilityHyperbolic microchannelMicrofluidic devicesRBCWBCBlood flow presents several interesting phenomena in microcirculation that can be used to develop microfluidic devices capable to promote blood cells separation and analysis in continuous flow. In the last decade there have been numerous microfluidic studies focused on the deformation of red blood cells (RBCs) flowing through geometries mimicking microvessels. In contrast, studies focusing on the deformation of white blood cells (WBCs) are scarce despite this phenomenon often happens in the microcirculation. In this work, we present a novel integrative microfluidic device able to perform continuous separation of a desired amount of blood cells, without clogging or jamming, and at the same time, capable to assess the deformation index (DI) of both WBCs and RBCs. To determine the DI of both WBCs and RBCs, a hyperbolic converging microchannel was used, as well as a suitable image analysis technique to measure the DIs of these blood cells along the regions of interest. The results show that the WBCs have a much lower deformability than RBCs when subjected to the same in vitro flow conditions, which is directly related to their cytoskeleton and nucleus contents. The proposed strategy can be easily transformed into a simple and inexpensive diagnostic microfluidic system to simultaneously separate and assess blood cells deformability.The authors acknowledge the financial support provided by PTDC/SAU-ENB/116929/2010 and EXPL/EMS-SIS/ 2215/2013 from FCT (Fundação para a Ciência e a Tecnologia), COMPETE, QREN and European Union (FEDER). R. O. Rodrigues, D. Pinho and V. Faustino acknowledge respectively, the PhD scholarships SFRH/BD/97658/2013, SFRH/BD/89077/2012 and SFRH/BD/99696/ 2014 granted by FCT. The authors would also like to thank Dr. Ângela Fernandes for providing the blood samples and Dr. Ricardo Calhelha for supplying the tissue culture medium used in this work.Biblioteca Digital do IPBRodrigues, Raquel OliveiraPinho, DianaFaustino, VeraLima, Rui A.2018-01-19T10:00:00Z20152015-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10198/16100engRodrigues, Raquel O.; Pinho, Diana; Faustino, Vera; Lima, Rui (2015). A simple microfluidic device for the deformability assessment of blood cells in a continuous flow. Biomedical Microdevices. ISSN 1387-2176. 17:108, p. 1-91387-217610.1007/s10544-015-0014-2info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-11-21T10:37:13Zoai:bibliotecadigital.ipb.pt:10198/16100Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T23:05:25.305676Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv A simple microfluidic device for the deformability assessment of blood cells in a continuous flow
title A simple microfluidic device for the deformability assessment of blood cells in a continuous flow
spellingShingle A simple microfluidic device for the deformability assessment of blood cells in a continuous flow
Rodrigues, Raquel Oliveira
Blood on chips
Cell separation and deformability
Hyperbolic microchannel
Microfluidic devices
RBC
WBC
title_short A simple microfluidic device for the deformability assessment of blood cells in a continuous flow
title_full A simple microfluidic device for the deformability assessment of blood cells in a continuous flow
title_fullStr A simple microfluidic device for the deformability assessment of blood cells in a continuous flow
title_full_unstemmed A simple microfluidic device for the deformability assessment of blood cells in a continuous flow
title_sort A simple microfluidic device for the deformability assessment of blood cells in a continuous flow
author Rodrigues, Raquel Oliveira
author_facet Rodrigues, Raquel Oliveira
Pinho, Diana
Faustino, Vera
Lima, Rui A.
author_role author
author2 Pinho, Diana
Faustino, Vera
Lima, Rui A.
author2_role author
author
author
dc.contributor.none.fl_str_mv Biblioteca Digital do IPB
dc.contributor.author.fl_str_mv Rodrigues, Raquel Oliveira
Pinho, Diana
Faustino, Vera
Lima, Rui A.
dc.subject.por.fl_str_mv Blood on chips
Cell separation and deformability
Hyperbolic microchannel
Microfluidic devices
RBC
WBC
topic Blood on chips
Cell separation and deformability
Hyperbolic microchannel
Microfluidic devices
RBC
WBC
description Blood flow presents several interesting phenomena in microcirculation that can be used to develop microfluidic devices capable to promote blood cells separation and analysis in continuous flow. In the last decade there have been numerous microfluidic studies focused on the deformation of red blood cells (RBCs) flowing through geometries mimicking microvessels. In contrast, studies focusing on the deformation of white blood cells (WBCs) are scarce despite this phenomenon often happens in the microcirculation. In this work, we present a novel integrative microfluidic device able to perform continuous separation of a desired amount of blood cells, without clogging or jamming, and at the same time, capable to assess the deformation index (DI) of both WBCs and RBCs. To determine the DI of both WBCs and RBCs, a hyperbolic converging microchannel was used, as well as a suitable image analysis technique to measure the DIs of these blood cells along the regions of interest. The results show that the WBCs have a much lower deformability than RBCs when subjected to the same in vitro flow conditions, which is directly related to their cytoskeleton and nucleus contents. The proposed strategy can be easily transformed into a simple and inexpensive diagnostic microfluidic system to simultaneously separate and assess blood cells deformability.
publishDate 2015
dc.date.none.fl_str_mv 2015
2015-01-01T00:00:00Z
2018-01-19T10:00:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10198/16100
url http://hdl.handle.net/10198/16100
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Rodrigues, Raquel O.; Pinho, Diana; Faustino, Vera; Lima, Rui (2015). A simple microfluidic device for the deformability assessment of blood cells in a continuous flow. Biomedical Microdevices. ISSN 1387-2176. 17:108, p. 1-9
1387-2176
10.1007/s10544-015-0014-2
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