Impact of a novel sugarcane straw extract-based ingredient on skin microbiota via a new preclinical in vitro model
Autor(a) principal: | |
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Data de Publicação: | 2023 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10400.14/43482 |
Resumo: | During cosmetic product development, the impact on the skin microbiota needs to be evaluated, as it plays an important role in skin health. Clinical studies are frequently used to assess cosmetic effects, but are expensive, time-consuming and require finished ingredients, which may be a limiting factor. Thus, this study had two main objectives, the development of a preclinical in vitro model for the evaluation of the effect of cosmetic ingredients on the skin microbiota, and its use to evaluate a novel sugarcane straw extract-based ingredient. In the development of the preclinical in vitro model, the microbiota incubation time and atmospheric conditions were optimized. The model was validated using a benchmark ingredient with reported in vivo effects on skin microbiota. Then, the new sugarcane straw extract-based ingredient was tested in the model, using skin microbiota samples from 12 healthy female volunteers. The impact on microbial groups was analyzed using qPCR and next-generation sequencing. The best conditions for the in vitro model were 24 h incubation under aerobic conditions. Furthermore, the results obtained with the benchmark ingredient agreed with those obtained in vivo, thus validating our model. Sugarcane straw extract-based ingredient was found to have no effect on community α-diversity, however it appears to affect S. epidermidis. In conclusion, the developed model can be used as a tool to assess the impact of novel cosmetic ingredients on skin microbiota. Also, the novel ingredient seems to have an impact on commensal S. epidermidis. |
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Impact of a novel sugarcane straw extract-based ingredient on skin microbiota via a new preclinical in vitro modelCosmeticIn vitro modelNatural ingredientsQPCRSkin microbiotaDuring cosmetic product development, the impact on the skin microbiota needs to be evaluated, as it plays an important role in skin health. Clinical studies are frequently used to assess cosmetic effects, but are expensive, time-consuming and require finished ingredients, which may be a limiting factor. Thus, this study had two main objectives, the development of a preclinical in vitro model for the evaluation of the effect of cosmetic ingredients on the skin microbiota, and its use to evaluate a novel sugarcane straw extract-based ingredient. In the development of the preclinical in vitro model, the microbiota incubation time and atmospheric conditions were optimized. The model was validated using a benchmark ingredient with reported in vivo effects on skin microbiota. Then, the new sugarcane straw extract-based ingredient was tested in the model, using skin microbiota samples from 12 healthy female volunteers. The impact on microbial groups was analyzed using qPCR and next-generation sequencing. The best conditions for the in vitro model were 24 h incubation under aerobic conditions. Furthermore, the results obtained with the benchmark ingredient agreed with those obtained in vivo, thus validating our model. Sugarcane straw extract-based ingredient was found to have no effect on community α-diversity, however it appears to affect S. epidermidis. In conclusion, the developed model can be used as a tool to assess the impact of novel cosmetic ingredients on skin microbiota. Also, the novel ingredient seems to have an impact on commensal S. epidermidis.Veritati - Repositório Institucional da Universidade Católica PortuguesaCarvalho, Maria JoãoPinto-Ribeiro, InêsCastro, CláudiaPedrosa, Sílvia SantosOliveira, Ana L. S.Pintado, ManuelaMadureira, Ana Raquel2024-01-04T12:50:58Z2023-122023-12-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10400.14/43482eng2950-194610.1016/j.microb.2023.100017info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-01-09T01:37:40Zoai:repositorio.ucp.pt:10400.14/43482Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T01:30:52.809853Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Impact of a novel sugarcane straw extract-based ingredient on skin microbiota via a new preclinical in vitro model |
title |
Impact of a novel sugarcane straw extract-based ingredient on skin microbiota via a new preclinical in vitro model |
spellingShingle |
Impact of a novel sugarcane straw extract-based ingredient on skin microbiota via a new preclinical in vitro model Carvalho, Maria João Cosmetic In vitro model Natural ingredients QPCR Skin microbiota |
title_short |
Impact of a novel sugarcane straw extract-based ingredient on skin microbiota via a new preclinical in vitro model |
title_full |
Impact of a novel sugarcane straw extract-based ingredient on skin microbiota via a new preclinical in vitro model |
title_fullStr |
Impact of a novel sugarcane straw extract-based ingredient on skin microbiota via a new preclinical in vitro model |
title_full_unstemmed |
Impact of a novel sugarcane straw extract-based ingredient on skin microbiota via a new preclinical in vitro model |
title_sort |
Impact of a novel sugarcane straw extract-based ingredient on skin microbiota via a new preclinical in vitro model |
author |
Carvalho, Maria João |
author_facet |
Carvalho, Maria João Pinto-Ribeiro, Inês Castro, Cláudia Pedrosa, Sílvia Santos Oliveira, Ana L. S. Pintado, Manuela Madureira, Ana Raquel |
author_role |
author |
author2 |
Pinto-Ribeiro, Inês Castro, Cláudia Pedrosa, Sílvia Santos Oliveira, Ana L. S. Pintado, Manuela Madureira, Ana Raquel |
author2_role |
author author author author author author |
dc.contributor.none.fl_str_mv |
Veritati - Repositório Institucional da Universidade Católica Portuguesa |
dc.contributor.author.fl_str_mv |
Carvalho, Maria João Pinto-Ribeiro, Inês Castro, Cláudia Pedrosa, Sílvia Santos Oliveira, Ana L. S. Pintado, Manuela Madureira, Ana Raquel |
dc.subject.por.fl_str_mv |
Cosmetic In vitro model Natural ingredients QPCR Skin microbiota |
topic |
Cosmetic In vitro model Natural ingredients QPCR Skin microbiota |
description |
During cosmetic product development, the impact on the skin microbiota needs to be evaluated, as it plays an important role in skin health. Clinical studies are frequently used to assess cosmetic effects, but are expensive, time-consuming and require finished ingredients, which may be a limiting factor. Thus, this study had two main objectives, the development of a preclinical in vitro model for the evaluation of the effect of cosmetic ingredients on the skin microbiota, and its use to evaluate a novel sugarcane straw extract-based ingredient. In the development of the preclinical in vitro model, the microbiota incubation time and atmospheric conditions were optimized. The model was validated using a benchmark ingredient with reported in vivo effects on skin microbiota. Then, the new sugarcane straw extract-based ingredient was tested in the model, using skin microbiota samples from 12 healthy female volunteers. The impact on microbial groups was analyzed using qPCR and next-generation sequencing. The best conditions for the in vitro model were 24 h incubation under aerobic conditions. Furthermore, the results obtained with the benchmark ingredient agreed with those obtained in vivo, thus validating our model. Sugarcane straw extract-based ingredient was found to have no effect on community α-diversity, however it appears to affect S. epidermidis. In conclusion, the developed model can be used as a tool to assess the impact of novel cosmetic ingredients on skin microbiota. Also, the novel ingredient seems to have an impact on commensal S. epidermidis. |
publishDate |
2023 |
dc.date.none.fl_str_mv |
2023-12 2023-12-01T00:00:00Z 2024-01-04T12:50:58Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10400.14/43482 |
url |
http://hdl.handle.net/10400.14/43482 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
2950-1946 10.1016/j.microb.2023.100017 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
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Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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RCAAP |
institution |
RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
collection |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository.name.fl_str_mv |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
repository.mail.fl_str_mv |
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1799136790443982848 |