Transient HES5 Activity Instructs Mesodermal Cells toward a Cardiac Fate
Autor(a) principal: | |
---|---|
Data de Publicação: | 2017 |
Outros Autores: | , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10316/108395 https://doi.org/10.1016/j.stemcr.2017.05.025 |
Resumo: | Notch signaling plays a role in specifying a cardiac fate but the downstream effectors remain unknown. In this study we implicate the Notch downstream effector HES5 in cardiogenesis. We show transient Hes5 expression in early mesoderm of gastrulating embryos and demonstrate, by loss and gain-of-function experiments in mouse embryonic stem cells, that HES5 favors cardiac over primitive erythroid fate. Hes5 overexpression promotes upregulation of the cardiac gene Isl1, while the hematopoietic regulator Scl is downregulated. Moreover, whereas a pulse of Hes5 instructs cardiac commitment, sustained expression after lineage specification impairs progression of differentiation to contracting cardiomyocytes. These findings establish a role for HES5 in cardiogenesis and provide insights into the early cardiac molecular network. |
id |
RCAP_e3df6fcab2d213ca99a1265f2576ca41 |
---|---|
oai_identifier_str |
oai:estudogeral.uc.pt:10316/108395 |
network_acronym_str |
RCAP |
network_name_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository_id_str |
7160 |
spelling |
Transient HES5 Activity Instructs Mesodermal Cells toward a Cardiac FateHes5; cardiac fate specification; nascent mesoderm; notch signaling pathwayAnimalsBasic Helix-Loop-Helix Transcription FactorsCell LineCell ProliferationErythropoiesisGastrulationGene Expression Regulation, DevelopmentalGene Knockdown TechniquesMesodermMiceMouse Embryonic Stem CellsMyocytes, CardiacRepressor ProteinsSignal TransductionCell DifferentiationNotch signaling plays a role in specifying a cardiac fate but the downstream effectors remain unknown. In this study we implicate the Notch downstream effector HES5 in cardiogenesis. We show transient Hes5 expression in early mesoderm of gastrulating embryos and demonstrate, by loss and gain-of-function experiments in mouse embryonic stem cells, that HES5 favors cardiac over primitive erythroid fate. Hes5 overexpression promotes upregulation of the cardiac gene Isl1, while the hematopoietic regulator Scl is downregulated. Moreover, whereas a pulse of Hes5 instructs cardiac commitment, sustained expression after lineage specification impairs progression of differentiation to contracting cardiomyocytes. These findings establish a role for HES5 in cardiogenesis and provide insights into the early cardiac molecular network.Elsevier2017-07-11info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://hdl.handle.net/10316/108395http://hdl.handle.net/10316/108395https://doi.org/10.1016/j.stemcr.2017.05.025eng22136711Freire, Ana G.Waghray, AvinashSoares-da-Silva, FranciscaResende, Tatiana P.Lee, Dung-FangPereira, Carlos FilipeNascimento, Diana S.Lemischka, Ihor R.Pinto-do-Ó, Perpétuainfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-08-29T08:01:16Zoai:estudogeral.uc.pt:10316/108395Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T21:24:41.726034Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Transient HES5 Activity Instructs Mesodermal Cells toward a Cardiac Fate |
title |
Transient HES5 Activity Instructs Mesodermal Cells toward a Cardiac Fate |
spellingShingle |
Transient HES5 Activity Instructs Mesodermal Cells toward a Cardiac Fate Freire, Ana G. Hes5; cardiac fate specification; nascent mesoderm; notch signaling pathway Animals Basic Helix-Loop-Helix Transcription Factors Cell Line Cell Proliferation Erythropoiesis Gastrulation Gene Expression Regulation, Developmental Gene Knockdown Techniques Mesoderm Mice Mouse Embryonic Stem Cells Myocytes, Cardiac Repressor Proteins Signal Transduction Cell Differentiation |
title_short |
Transient HES5 Activity Instructs Mesodermal Cells toward a Cardiac Fate |
title_full |
Transient HES5 Activity Instructs Mesodermal Cells toward a Cardiac Fate |
title_fullStr |
Transient HES5 Activity Instructs Mesodermal Cells toward a Cardiac Fate |
title_full_unstemmed |
Transient HES5 Activity Instructs Mesodermal Cells toward a Cardiac Fate |
title_sort |
Transient HES5 Activity Instructs Mesodermal Cells toward a Cardiac Fate |
author |
Freire, Ana G. |
author_facet |
Freire, Ana G. Waghray, Avinash Soares-da-Silva, Francisca Resende, Tatiana P. Lee, Dung-Fang Pereira, Carlos Filipe Nascimento, Diana S. Lemischka, Ihor R. Pinto-do-Ó, Perpétua |
author_role |
author |
author2 |
Waghray, Avinash Soares-da-Silva, Francisca Resende, Tatiana P. Lee, Dung-Fang Pereira, Carlos Filipe Nascimento, Diana S. Lemischka, Ihor R. Pinto-do-Ó, Perpétua |
author2_role |
author author author author author author author author |
dc.contributor.author.fl_str_mv |
Freire, Ana G. Waghray, Avinash Soares-da-Silva, Francisca Resende, Tatiana P. Lee, Dung-Fang Pereira, Carlos Filipe Nascimento, Diana S. Lemischka, Ihor R. Pinto-do-Ó, Perpétua |
dc.subject.por.fl_str_mv |
Hes5; cardiac fate specification; nascent mesoderm; notch signaling pathway Animals Basic Helix-Loop-Helix Transcription Factors Cell Line Cell Proliferation Erythropoiesis Gastrulation Gene Expression Regulation, Developmental Gene Knockdown Techniques Mesoderm Mice Mouse Embryonic Stem Cells Myocytes, Cardiac Repressor Proteins Signal Transduction Cell Differentiation |
topic |
Hes5; cardiac fate specification; nascent mesoderm; notch signaling pathway Animals Basic Helix-Loop-Helix Transcription Factors Cell Line Cell Proliferation Erythropoiesis Gastrulation Gene Expression Regulation, Developmental Gene Knockdown Techniques Mesoderm Mice Mouse Embryonic Stem Cells Myocytes, Cardiac Repressor Proteins Signal Transduction Cell Differentiation |
description |
Notch signaling plays a role in specifying a cardiac fate but the downstream effectors remain unknown. In this study we implicate the Notch downstream effector HES5 in cardiogenesis. We show transient Hes5 expression in early mesoderm of gastrulating embryos and demonstrate, by loss and gain-of-function experiments in mouse embryonic stem cells, that HES5 favors cardiac over primitive erythroid fate. Hes5 overexpression promotes upregulation of the cardiac gene Isl1, while the hematopoietic regulator Scl is downregulated. Moreover, whereas a pulse of Hes5 instructs cardiac commitment, sustained expression after lineage specification impairs progression of differentiation to contracting cardiomyocytes. These findings establish a role for HES5 in cardiogenesis and provide insights into the early cardiac molecular network. |
publishDate |
2017 |
dc.date.none.fl_str_mv |
2017-07-11 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10316/108395 http://hdl.handle.net/10316/108395 https://doi.org/10.1016/j.stemcr.2017.05.025 |
url |
http://hdl.handle.net/10316/108395 https://doi.org/10.1016/j.stemcr.2017.05.025 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
22136711 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.publisher.none.fl_str_mv |
Elsevier |
publisher.none.fl_str_mv |
Elsevier |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
instname_str |
Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
instacron_str |
RCAAP |
institution |
RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
collection |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository.name.fl_str_mv |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
repository.mail.fl_str_mv |
|
_version_ |
1817552496865837056 |