Transient HES5 Activity Instructs Mesodermal Cells toward a Cardiac Fate

Detalhes bibliográficos
Autor(a) principal: Freire, Ana G.
Data de Publicação: 2017
Outros Autores: Waghray, Avinash, Soares-da-Silva, Francisca, Resende, Tatiana P., Lee, Dung-Fang, Pereira, Carlos Filipe, Nascimento, Diana S., Lemischka, Ihor R., Pinto-do-Ó, Perpétua
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10316/108395
https://doi.org/10.1016/j.stemcr.2017.05.025
Resumo: Notch signaling plays a role in specifying a cardiac fate but the downstream effectors remain unknown. In this study we implicate the Notch downstream effector HES5 in cardiogenesis. We show transient Hes5 expression in early mesoderm of gastrulating embryos and demonstrate, by loss and gain-of-function experiments in mouse embryonic stem cells, that HES5 favors cardiac over primitive erythroid fate. Hes5 overexpression promotes upregulation of the cardiac gene Isl1, while the hematopoietic regulator Scl is downregulated. Moreover, whereas a pulse of Hes5 instructs cardiac commitment, sustained expression after lineage specification impairs progression of differentiation to contracting cardiomyocytes. These findings establish a role for HES5 in cardiogenesis and provide insights into the early cardiac molecular network.
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spelling Transient HES5 Activity Instructs Mesodermal Cells toward a Cardiac FateHes5; cardiac fate specification; nascent mesoderm; notch signaling pathwayAnimalsBasic Helix-Loop-Helix Transcription FactorsCell LineCell ProliferationErythropoiesisGastrulationGene Expression Regulation, DevelopmentalGene Knockdown TechniquesMesodermMiceMouse Embryonic Stem CellsMyocytes, CardiacRepressor ProteinsSignal TransductionCell DifferentiationNotch signaling plays a role in specifying a cardiac fate but the downstream effectors remain unknown. In this study we implicate the Notch downstream effector HES5 in cardiogenesis. We show transient Hes5 expression in early mesoderm of gastrulating embryos and demonstrate, by loss and gain-of-function experiments in mouse embryonic stem cells, that HES5 favors cardiac over primitive erythroid fate. Hes5 overexpression promotes upregulation of the cardiac gene Isl1, while the hematopoietic regulator Scl is downregulated. Moreover, whereas a pulse of Hes5 instructs cardiac commitment, sustained expression after lineage specification impairs progression of differentiation to contracting cardiomyocytes. These findings establish a role for HES5 in cardiogenesis and provide insights into the early cardiac molecular network.Elsevier2017-07-11info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://hdl.handle.net/10316/108395http://hdl.handle.net/10316/108395https://doi.org/10.1016/j.stemcr.2017.05.025eng22136711Freire, Ana G.Waghray, AvinashSoares-da-Silva, FranciscaResende, Tatiana P.Lee, Dung-FangPereira, Carlos FilipeNascimento, Diana S.Lemischka, Ihor R.Pinto-do-Ó, Perpétuainfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-08-29T08:01:16Zoai:estudogeral.uc.pt:10316/108395Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T21:24:41.726034Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Transient HES5 Activity Instructs Mesodermal Cells toward a Cardiac Fate
title Transient HES5 Activity Instructs Mesodermal Cells toward a Cardiac Fate
spellingShingle Transient HES5 Activity Instructs Mesodermal Cells toward a Cardiac Fate
Freire, Ana G.
Hes5; cardiac fate specification; nascent mesoderm; notch signaling pathway
Animals
Basic Helix-Loop-Helix Transcription Factors
Cell Line
Cell Proliferation
Erythropoiesis
Gastrulation
Gene Expression Regulation, Developmental
Gene Knockdown Techniques
Mesoderm
Mice
Mouse Embryonic Stem Cells
Myocytes, Cardiac
Repressor Proteins
Signal Transduction
Cell Differentiation
title_short Transient HES5 Activity Instructs Mesodermal Cells toward a Cardiac Fate
title_full Transient HES5 Activity Instructs Mesodermal Cells toward a Cardiac Fate
title_fullStr Transient HES5 Activity Instructs Mesodermal Cells toward a Cardiac Fate
title_full_unstemmed Transient HES5 Activity Instructs Mesodermal Cells toward a Cardiac Fate
title_sort Transient HES5 Activity Instructs Mesodermal Cells toward a Cardiac Fate
author Freire, Ana G.
author_facet Freire, Ana G.
Waghray, Avinash
Soares-da-Silva, Francisca
Resende, Tatiana P.
Lee, Dung-Fang
Pereira, Carlos Filipe
Nascimento, Diana S.
Lemischka, Ihor R.
Pinto-do-Ó, Perpétua
author_role author
author2 Waghray, Avinash
Soares-da-Silva, Francisca
Resende, Tatiana P.
Lee, Dung-Fang
Pereira, Carlos Filipe
Nascimento, Diana S.
Lemischka, Ihor R.
Pinto-do-Ó, Perpétua
author2_role author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Freire, Ana G.
Waghray, Avinash
Soares-da-Silva, Francisca
Resende, Tatiana P.
Lee, Dung-Fang
Pereira, Carlos Filipe
Nascimento, Diana S.
Lemischka, Ihor R.
Pinto-do-Ó, Perpétua
dc.subject.por.fl_str_mv Hes5; cardiac fate specification; nascent mesoderm; notch signaling pathway
Animals
Basic Helix-Loop-Helix Transcription Factors
Cell Line
Cell Proliferation
Erythropoiesis
Gastrulation
Gene Expression Regulation, Developmental
Gene Knockdown Techniques
Mesoderm
Mice
Mouse Embryonic Stem Cells
Myocytes, Cardiac
Repressor Proteins
Signal Transduction
Cell Differentiation
topic Hes5; cardiac fate specification; nascent mesoderm; notch signaling pathway
Animals
Basic Helix-Loop-Helix Transcription Factors
Cell Line
Cell Proliferation
Erythropoiesis
Gastrulation
Gene Expression Regulation, Developmental
Gene Knockdown Techniques
Mesoderm
Mice
Mouse Embryonic Stem Cells
Myocytes, Cardiac
Repressor Proteins
Signal Transduction
Cell Differentiation
description Notch signaling plays a role in specifying a cardiac fate but the downstream effectors remain unknown. In this study we implicate the Notch downstream effector HES5 in cardiogenesis. We show transient Hes5 expression in early mesoderm of gastrulating embryos and demonstrate, by loss and gain-of-function experiments in mouse embryonic stem cells, that HES5 favors cardiac over primitive erythroid fate. Hes5 overexpression promotes upregulation of the cardiac gene Isl1, while the hematopoietic regulator Scl is downregulated. Moreover, whereas a pulse of Hes5 instructs cardiac commitment, sustained expression after lineage specification impairs progression of differentiation to contracting cardiomyocytes. These findings establish a role for HES5 in cardiogenesis and provide insights into the early cardiac molecular network.
publishDate 2017
dc.date.none.fl_str_mv 2017-07-11
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10316/108395
http://hdl.handle.net/10316/108395
https://doi.org/10.1016/j.stemcr.2017.05.025
url http://hdl.handle.net/10316/108395
https://doi.org/10.1016/j.stemcr.2017.05.025
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 22136711
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
repository.mail.fl_str_mv
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