A new method for yeast phagocytosis analysis by flow cytometry

Detalhes bibliográficos
Autor(a) principal: Carneiro, Maria Catarina Santos
Data de Publicação: 2014
Outros Autores: Vaz, Catarina Oliveira, Pais, Célia, Sampaio, Paula, Pereira, Joana Isabel Carvalho
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/1822/50943
Resumo: Herein we developed a method based on the quenching effect of propidium iodide over Sytox-Green fluorescence to assess yeast phagocytosis by flow cytometry. It allows accurate quantification of living from dead phagocytes; internalized from non-internalized cells, maintaining yeast fluorescence within phagocytes; and the different associations between phagocytes and fungal cells. (C) 2014 Elsevier B.V. All rights reserved.
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spelling A new method for yeast phagocytosis analysis by flow cytometryCandida sppFlow cytometrySytox greenPropidium iodidePhagocytosisPAMPsScience & TechnologyHerein we developed a method based on the quenching effect of propidium iodide over Sytox-Green fluorescence to assess yeast phagocytosis by flow cytometry. It allows accurate quantification of living from dead phagocytes; internalized from non-internalized cells, maintaining yeast fluorescence within phagocytes; and the different associations between phagocytes and fungal cells. (C) 2014 Elsevier B.V. All rights reserved.This research was supported by FEDER Funds through the Operational Programme COMPETE and national funds through the Fundação para a Ciência e Tecnologia (FCT), in the scope of project PEst-OE/BIA/ UI4050/2014.info:eu-repo/semantics/publishedVersionElsevierUniversidade do MinhoCarneiro, Maria Catarina SantosVaz, Catarina OliveiraPais, CéliaSampaio, PaulaPereira, Joana Isabel Carvalho20142014-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/1822/50943eng0167-701210.1016/j.mimet.2014.03.01324704495info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-21T12:01:16Zoai:repositorium.sdum.uminho.pt:1822/50943Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T18:51:11.025249Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv A new method for yeast phagocytosis analysis by flow cytometry
title A new method for yeast phagocytosis analysis by flow cytometry
spellingShingle A new method for yeast phagocytosis analysis by flow cytometry
Carneiro, Maria Catarina Santos
Candida spp
Flow cytometry
Sytox green
Propidium iodide
Phagocytosis
PAMPs
Science & Technology
title_short A new method for yeast phagocytosis analysis by flow cytometry
title_full A new method for yeast phagocytosis analysis by flow cytometry
title_fullStr A new method for yeast phagocytosis analysis by flow cytometry
title_full_unstemmed A new method for yeast phagocytosis analysis by flow cytometry
title_sort A new method for yeast phagocytosis analysis by flow cytometry
author Carneiro, Maria Catarina Santos
author_facet Carneiro, Maria Catarina Santos
Vaz, Catarina Oliveira
Pais, Célia
Sampaio, Paula
Pereira, Joana Isabel Carvalho
author_role author
author2 Vaz, Catarina Oliveira
Pais, Célia
Sampaio, Paula
Pereira, Joana Isabel Carvalho
author2_role author
author
author
author
dc.contributor.none.fl_str_mv Universidade do Minho
dc.contributor.author.fl_str_mv Carneiro, Maria Catarina Santos
Vaz, Catarina Oliveira
Pais, Célia
Sampaio, Paula
Pereira, Joana Isabel Carvalho
dc.subject.por.fl_str_mv Candida spp
Flow cytometry
Sytox green
Propidium iodide
Phagocytosis
PAMPs
Science & Technology
topic Candida spp
Flow cytometry
Sytox green
Propidium iodide
Phagocytosis
PAMPs
Science & Technology
description Herein we developed a method based on the quenching effect of propidium iodide over Sytox-Green fluorescence to assess yeast phagocytosis by flow cytometry. It allows accurate quantification of living from dead phagocytes; internalized from non-internalized cells, maintaining yeast fluorescence within phagocytes; and the different associations between phagocytes and fungal cells. (C) 2014 Elsevier B.V. All rights reserved.
publishDate 2014
dc.date.none.fl_str_mv 2014
2014-01-01T00:00:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/1822/50943
url http://hdl.handle.net/1822/50943
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 0167-7012
10.1016/j.mimet.2014.03.013
24704495
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
repository.mail.fl_str_mv
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