Production of hyperimmune egg samples followed by aqueous biphasic systems fractionation

Detalhes bibliográficos
Autor(a) principal: Silva, Diana Patrícia Gil da
Data de Publicação: 2015
Tipo de documento: Dissertação
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10773/15463
Resumo: Antibodies are glycoproteins that belong to the family of immunoglobulins (Ig). They are found in plasma and extracellular fluids of vertebrates and are produced by B cells to identify and to neutralize pathogens and foreign molecules (antigens) in our body. The production of monoclonal and polyclonal antibodies and their ability to bind to a specific antigen, allows their use in quantitative and/or qualitative analyses of specific antigens, in purification methods and in modulating physiological effects in research, diagnosis or therapy. Hen antibodies (IgY) have been studied for such type of applications because they can be produced at a lower cost and in larger amounts. The production of IgY for use in passive immunization has been suggested because there are many antibioticresistant microorganisms, diseases that do not respond to the applied drugs and individuals who are unable to respond to vaccination. The major objective of this work consists on the injection of hens with specific antigens (produced with the aid of fusion tags) which leads to a humoral immune response and to the production of hyperimmune eggs, which were followed a long time. Finally, the stability of IgY was evaluated in aqueous solutions of salts and polymers that could be used in the creation of aqueous biphasic systems for purification purposes.
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spelling Production of hyperimmune egg samples followed by aqueous biphasic systems fractionationBioquímicaImunoglobulinasSoluções iónicasOvos - ProduçãoAntibodies are glycoproteins that belong to the family of immunoglobulins (Ig). They are found in plasma and extracellular fluids of vertebrates and are produced by B cells to identify and to neutralize pathogens and foreign molecules (antigens) in our body. The production of monoclonal and polyclonal antibodies and their ability to bind to a specific antigen, allows their use in quantitative and/or qualitative analyses of specific antigens, in purification methods and in modulating physiological effects in research, diagnosis or therapy. Hen antibodies (IgY) have been studied for such type of applications because they can be produced at a lower cost and in larger amounts. The production of IgY for use in passive immunization has been suggested because there are many antibioticresistant microorganisms, diseases that do not respond to the applied drugs and individuals who are unable to respond to vaccination. The major objective of this work consists on the injection of hens with specific antigens (produced with the aid of fusion tags) which leads to a humoral immune response and to the production of hyperimmune eggs, which were followed a long time. Finally, the stability of IgY was evaluated in aqueous solutions of salts and polymers that could be used in the creation of aqueous biphasic systems for purification purposes.Os anticorpos pertencem à família das Imunoglobulinas (Ig), e que se encontram no plasma e no fluído extracelular dos vertebrados, sendo produzidos pelas células B de modo a identificar e neutralizar as moléculas estranhas e patogénicas (antigénios) ao nosso organismo. A produção de anticorpos monoclonais e policlonais e a sua capacidade para se ligarem a antigénios específicos possibilitou a sua aplicação em análises quantitativas e/ou qualitativas, em métodos de purificação de antigénios e na modulação de efeitos fisiológicos em investigação, de diagnóstico ou terapêutica. Os anticorpos das aves (IgY) têm sido muito estudados neste tipo de aplicações por serem uma fonte mais económica e existirem em maior quantidade. A produção de IgY para aplicação em imunização passiva tem vindo a ser estudada nos últimos anos, e isto deve-se ao fato de existirem muitos microrganismos resistentes a antibióticos, doenças que não reagem aos fármacos aplicados e de indivíduos que são incapazes de responder ao método de vacinação tradicional. Para a produção de anticorpos específicos, expõem-se a ave a um determinado antigénio, através de injeção, levando posteriormente a uma resposta imunitária humoral e assim à produção de ovos hiperimunes. O grande objetivo deste trabalho consistiu na produção de ovos hiperimunes, na qual se acompanhou o perfil dos mesmos ao longo do tempo, seguido da avaliação da estabilidade de IgY em soluções aquosas de sais e polímeros utilizados em sistemas aquosos bifásicos que poderão ser estratégias promissoras para a sua purificação.Universidade de Aveiro2015-09-242015-09-24T00:00:00Z2020-09-24T20:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesisapplication/pdfhttp://hdl.handle.net/10773/15463TID:201572516engSilva, Diana Patrícia Gil dainfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-02-22T11:28:35Zoai:ria.ua.pt:10773/15463Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T02:50:50.392042Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Production of hyperimmune egg samples followed by aqueous biphasic systems fractionation
title Production of hyperimmune egg samples followed by aqueous biphasic systems fractionation
spellingShingle Production of hyperimmune egg samples followed by aqueous biphasic systems fractionation
Silva, Diana Patrícia Gil da
Bioquímica
Imunoglobulinas
Soluções iónicas
Ovos - Produção
title_short Production of hyperimmune egg samples followed by aqueous biphasic systems fractionation
title_full Production of hyperimmune egg samples followed by aqueous biphasic systems fractionation
title_fullStr Production of hyperimmune egg samples followed by aqueous biphasic systems fractionation
title_full_unstemmed Production of hyperimmune egg samples followed by aqueous biphasic systems fractionation
title_sort Production of hyperimmune egg samples followed by aqueous biphasic systems fractionation
author Silva, Diana Patrícia Gil da
author_facet Silva, Diana Patrícia Gil da
author_role author
dc.contributor.author.fl_str_mv Silva, Diana Patrícia Gil da
dc.subject.por.fl_str_mv Bioquímica
Imunoglobulinas
Soluções iónicas
Ovos - Produção
topic Bioquímica
Imunoglobulinas
Soluções iónicas
Ovos - Produção
description Antibodies are glycoproteins that belong to the family of immunoglobulins (Ig). They are found in plasma and extracellular fluids of vertebrates and are produced by B cells to identify and to neutralize pathogens and foreign molecules (antigens) in our body. The production of monoclonal and polyclonal antibodies and their ability to bind to a specific antigen, allows their use in quantitative and/or qualitative analyses of specific antigens, in purification methods and in modulating physiological effects in research, diagnosis or therapy. Hen antibodies (IgY) have been studied for such type of applications because they can be produced at a lower cost and in larger amounts. The production of IgY for use in passive immunization has been suggested because there are many antibioticresistant microorganisms, diseases that do not respond to the applied drugs and individuals who are unable to respond to vaccination. The major objective of this work consists on the injection of hens with specific antigens (produced with the aid of fusion tags) which leads to a humoral immune response and to the production of hyperimmune eggs, which were followed a long time. Finally, the stability of IgY was evaluated in aqueous solutions of salts and polymers that could be used in the creation of aqueous biphasic systems for purification purposes.
publishDate 2015
dc.date.none.fl_str_mv 2015-09-24
2015-09-24T00:00:00Z
2020-09-24T20:00:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/masterThesis
format masterThesis
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10773/15463
TID:201572516
url http://hdl.handle.net/10773/15463
identifier_str_mv TID:201572516
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Universidade de Aveiro
publisher.none.fl_str_mv Universidade de Aveiro
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
repository.mail.fl_str_mv
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