Ciliarmove

Detalhes bibliográficos
Autor(a) principal: Sampaio, Pedro
Data de Publicação: 2021
Outros Autores: da Silva, Mónica Ferro, Vale, Inês, Roxo-Rosa, Mónica, Pinto, Andreia, Constant, Carolina, Pereira, Luisa, Quintão, Carla M., Lopes, Susana S.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10362/144787
Resumo: This study was supported by the Fundação para a Ciência e a Tecnologia (PTDC/BEXBID/1411/ 2014 research grant). S.S. Lopes was funded by FCT Investigator IF/00951/2012, by NOVA Medical School and by FCT CEEC-IND 2018. P. Sampaio was funded by the PhD fellowship FCT: SFRH/BD/111611/2015. M. Roxo-Rosa was funded by the UID/Multi/04462/2013-LISBOA-01-0145-FEDER-007344 grant (iNOVA4Health). C.M. Quintão was funded by Fundação para a Ciência e Tecnologia (UID/FIS/04559/2013). S.S. Lopes participates in and acknowledge financial support from the COST Action BEAT-PCD (BM1407). S.S. Lopes received funding from project LysoCil funded by the European Union Horizon 2020 research and innovation under grant agreement No 811087. Funding information for this article has been deposited with the Crossref Funder Registry.
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spelling CiliarmoveNew software for evaluating ciliary beat frequency helps find novel mutations by a portuguese multidisciplinary team on primary ciliary dyskinesiaPulmonary and Respiratory MedicineThis study was supported by the Fundação para a Ciência e a Tecnologia (PTDC/BEXBID/1411/ 2014 research grant). S.S. Lopes was funded by FCT Investigator IF/00951/2012, by NOVA Medical School and by FCT CEEC-IND 2018. P. Sampaio was funded by the PhD fellowship FCT: SFRH/BD/111611/2015. M. Roxo-Rosa was funded by the UID/Multi/04462/2013-LISBOA-01-0145-FEDER-007344 grant (iNOVA4Health). C.M. Quintão was funded by Fundação para a Ciência e Tecnologia (UID/FIS/04559/2013). S.S. Lopes participates in and acknowledge financial support from the COST Action BEAT-PCD (BM1407). S.S. Lopes received funding from project LysoCil funded by the European Union Horizon 2020 research and innovation under grant agreement No 811087. Funding information for this article has been deposited with the Crossref Funder Registry.Evaluation of ciliary beat frequency (CBF) performed by high-speed videomicroscopy analysis (HVMA) is one of the techniques required for the correct diagnosis of primary ciliary dyskinesia (PCD). Currently, due to lack of open-source software, this technique is widely performed by visually counting the ciliary beatings per a given time-window. Our aim was to generate open-source, fast and intuitive software for evaluating CBF, validated in Portuguese PCD patients and healthy volunteers. Nasal brushings collected from 17 adult healthy volunteers and 34 PCD-referred subjects were recorded using HVMA. Evaluation of CBF was compared by two different methodologies: the new semi-automated computer software CiliarMove and the manual observation method using slow-motion movies. Clinical history, nasal nitric oxide and transmission electron microscopy were performed for diagnosis of PCD in the patient group. Genetic analysis was performed in a subset (n=8) of suspected PCD patients. The correlation coefficient between the two methods was R2=0.9895. The interval of CBF values obtained from the healthy control group (n=17) was 6.18–9.17 Hz at 25°C. In the PCD-excluded group (n=16), CBF ranged from 6.84 to 10.93 Hz and in the PCD group (n=18), CBF ranged from 0 to 14.30 Hz. We offer an automated open-source programme named CiliarMove, validated by the manual observation method in a healthy volunteer control group, a PCD-excluded group and a PCD-confirmed group. In our hands, comparisons between CBF intervals alone could discern between healthy and PCD groups in 78% of the cases.NOVA Medical School|Faculdade de Ciências Médicas (NMS|FCM)Centro de Estudos de Doenças Crónicas (CEDOC)iNOVA4Health - pólo NMSDF – Departamento de FísicaCTS - Centro de Tecnologia e SistemasLIBPhys-UNLRUNSampaio, Pedroda Silva, Mónica FerroVale, InêsRoxo-Rosa, MónicaPinto, AndreiaConstant, CarolinaPereira, LuisaQuintão, Carla M.Lopes, Susana S.2022-10-17T22:11:48Z20212021-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article12application/pdfhttp://hdl.handle.net/10362/144787eng2312-0541PURE: 47160234https://doi.org/10.1183/23120541.00792-2020info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-03-11T05:24:39Zoai:run.unl.pt:10362/144787Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T03:51:44.695482Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Ciliarmove
New software for evaluating ciliary beat frequency helps find novel mutations by a portuguese multidisciplinary team on primary ciliary dyskinesia
title Ciliarmove
spellingShingle Ciliarmove
Sampaio, Pedro
Pulmonary and Respiratory Medicine
title_short Ciliarmove
title_full Ciliarmove
title_fullStr Ciliarmove
title_full_unstemmed Ciliarmove
title_sort Ciliarmove
author Sampaio, Pedro
author_facet Sampaio, Pedro
da Silva, Mónica Ferro
Vale, Inês
Roxo-Rosa, Mónica
Pinto, Andreia
Constant, Carolina
Pereira, Luisa
Quintão, Carla M.
Lopes, Susana S.
author_role author
author2 da Silva, Mónica Ferro
Vale, Inês
Roxo-Rosa, Mónica
Pinto, Andreia
Constant, Carolina
Pereira, Luisa
Quintão, Carla M.
Lopes, Susana S.
author2_role author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv NOVA Medical School|Faculdade de Ciências Médicas (NMS|FCM)
Centro de Estudos de Doenças Crónicas (CEDOC)
iNOVA4Health - pólo NMS
DF – Departamento de Física
CTS - Centro de Tecnologia e Sistemas
LIBPhys-UNL
RUN
dc.contributor.author.fl_str_mv Sampaio, Pedro
da Silva, Mónica Ferro
Vale, Inês
Roxo-Rosa, Mónica
Pinto, Andreia
Constant, Carolina
Pereira, Luisa
Quintão, Carla M.
Lopes, Susana S.
dc.subject.por.fl_str_mv Pulmonary and Respiratory Medicine
topic Pulmonary and Respiratory Medicine
description This study was supported by the Fundação para a Ciência e a Tecnologia (PTDC/BEXBID/1411/ 2014 research grant). S.S. Lopes was funded by FCT Investigator IF/00951/2012, by NOVA Medical School and by FCT CEEC-IND 2018. P. Sampaio was funded by the PhD fellowship FCT: SFRH/BD/111611/2015. M. Roxo-Rosa was funded by the UID/Multi/04462/2013-LISBOA-01-0145-FEDER-007344 grant (iNOVA4Health). C.M. Quintão was funded by Fundação para a Ciência e Tecnologia (UID/FIS/04559/2013). S.S. Lopes participates in and acknowledge financial support from the COST Action BEAT-PCD (BM1407). S.S. Lopes received funding from project LysoCil funded by the European Union Horizon 2020 research and innovation under grant agreement No 811087. Funding information for this article has been deposited with the Crossref Funder Registry.
publishDate 2021
dc.date.none.fl_str_mv 2021
2021-01-01T00:00:00Z
2022-10-17T22:11:48Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10362/144787
url http://hdl.handle.net/10362/144787
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 2312-0541
PURE: 47160234
https://doi.org/10.1183/23120541.00792-2020
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 12
application/pdf
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instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
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reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
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