Ciliarmove
Autor(a) principal: | |
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Data de Publicação: | 2021 |
Outros Autores: | , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10362/144787 |
Resumo: | This study was supported by the Fundação para a Ciência e a Tecnologia (PTDC/BEXBID/1411/ 2014 research grant). S.S. Lopes was funded by FCT Investigator IF/00951/2012, by NOVA Medical School and by FCT CEEC-IND 2018. P. Sampaio was funded by the PhD fellowship FCT: SFRH/BD/111611/2015. M. Roxo-Rosa was funded by the UID/Multi/04462/2013-LISBOA-01-0145-FEDER-007344 grant (iNOVA4Health). C.M. Quintão was funded by Fundação para a Ciência e Tecnologia (UID/FIS/04559/2013). S.S. Lopes participates in and acknowledge financial support from the COST Action BEAT-PCD (BM1407). S.S. Lopes received funding from project LysoCil funded by the European Union Horizon 2020 research and innovation under grant agreement No 811087. Funding information for this article has been deposited with the Crossref Funder Registry. |
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CiliarmoveNew software for evaluating ciliary beat frequency helps find novel mutations by a portuguese multidisciplinary team on primary ciliary dyskinesiaPulmonary and Respiratory MedicineThis study was supported by the Fundação para a Ciência e a Tecnologia (PTDC/BEXBID/1411/ 2014 research grant). S.S. Lopes was funded by FCT Investigator IF/00951/2012, by NOVA Medical School and by FCT CEEC-IND 2018. P. Sampaio was funded by the PhD fellowship FCT: SFRH/BD/111611/2015. M. Roxo-Rosa was funded by the UID/Multi/04462/2013-LISBOA-01-0145-FEDER-007344 grant (iNOVA4Health). C.M. Quintão was funded by Fundação para a Ciência e Tecnologia (UID/FIS/04559/2013). S.S. Lopes participates in and acknowledge financial support from the COST Action BEAT-PCD (BM1407). S.S. Lopes received funding from project LysoCil funded by the European Union Horizon 2020 research and innovation under grant agreement No 811087. Funding information for this article has been deposited with the Crossref Funder Registry.Evaluation of ciliary beat frequency (CBF) performed by high-speed videomicroscopy analysis (HVMA) is one of the techniques required for the correct diagnosis of primary ciliary dyskinesia (PCD). Currently, due to lack of open-source software, this technique is widely performed by visually counting the ciliary beatings per a given time-window. Our aim was to generate open-source, fast and intuitive software for evaluating CBF, validated in Portuguese PCD patients and healthy volunteers. Nasal brushings collected from 17 adult healthy volunteers and 34 PCD-referred subjects were recorded using HVMA. Evaluation of CBF was compared by two different methodologies: the new semi-automated computer software CiliarMove and the manual observation method using slow-motion movies. Clinical history, nasal nitric oxide and transmission electron microscopy were performed for diagnosis of PCD in the patient group. Genetic analysis was performed in a subset (n=8) of suspected PCD patients. The correlation coefficient between the two methods was R2=0.9895. The interval of CBF values obtained from the healthy control group (n=17) was 6.18–9.17 Hz at 25°C. In the PCD-excluded group (n=16), CBF ranged from 6.84 to 10.93 Hz and in the PCD group (n=18), CBF ranged from 0 to 14.30 Hz. We offer an automated open-source programme named CiliarMove, validated by the manual observation method in a healthy volunteer control group, a PCD-excluded group and a PCD-confirmed group. In our hands, comparisons between CBF intervals alone could discern between healthy and PCD groups in 78% of the cases.NOVA Medical School|Faculdade de Ciências Médicas (NMS|FCM)Centro de Estudos de Doenças Crónicas (CEDOC)iNOVA4Health - pólo NMSDF – Departamento de FísicaCTS - Centro de Tecnologia e SistemasLIBPhys-UNLRUNSampaio, Pedroda Silva, Mónica FerroVale, InêsRoxo-Rosa, MónicaPinto, AndreiaConstant, CarolinaPereira, LuisaQuintão, Carla M.Lopes, Susana S.2022-10-17T22:11:48Z20212021-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article12application/pdfhttp://hdl.handle.net/10362/144787eng2312-0541PURE: 47160234https://doi.org/10.1183/23120541.00792-2020info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-03-11T05:24:39Zoai:run.unl.pt:10362/144787Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T03:51:44.695482Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Ciliarmove New software for evaluating ciliary beat frequency helps find novel mutations by a portuguese multidisciplinary team on primary ciliary dyskinesia |
title |
Ciliarmove |
spellingShingle |
Ciliarmove Sampaio, Pedro Pulmonary and Respiratory Medicine |
title_short |
Ciliarmove |
title_full |
Ciliarmove |
title_fullStr |
Ciliarmove |
title_full_unstemmed |
Ciliarmove |
title_sort |
Ciliarmove |
author |
Sampaio, Pedro |
author_facet |
Sampaio, Pedro da Silva, Mónica Ferro Vale, Inês Roxo-Rosa, Mónica Pinto, Andreia Constant, Carolina Pereira, Luisa Quintão, Carla M. Lopes, Susana S. |
author_role |
author |
author2 |
da Silva, Mónica Ferro Vale, Inês Roxo-Rosa, Mónica Pinto, Andreia Constant, Carolina Pereira, Luisa Quintão, Carla M. Lopes, Susana S. |
author2_role |
author author author author author author author author |
dc.contributor.none.fl_str_mv |
NOVA Medical School|Faculdade de Ciências Médicas (NMS|FCM) Centro de Estudos de Doenças Crónicas (CEDOC) iNOVA4Health - pólo NMS DF – Departamento de Física CTS - Centro de Tecnologia e Sistemas LIBPhys-UNL RUN |
dc.contributor.author.fl_str_mv |
Sampaio, Pedro da Silva, Mónica Ferro Vale, Inês Roxo-Rosa, Mónica Pinto, Andreia Constant, Carolina Pereira, Luisa Quintão, Carla M. Lopes, Susana S. |
dc.subject.por.fl_str_mv |
Pulmonary and Respiratory Medicine |
topic |
Pulmonary and Respiratory Medicine |
description |
This study was supported by the Fundação para a Ciência e a Tecnologia (PTDC/BEXBID/1411/ 2014 research grant). S.S. Lopes was funded by FCT Investigator IF/00951/2012, by NOVA Medical School and by FCT CEEC-IND 2018. P. Sampaio was funded by the PhD fellowship FCT: SFRH/BD/111611/2015. M. Roxo-Rosa was funded by the UID/Multi/04462/2013-LISBOA-01-0145-FEDER-007344 grant (iNOVA4Health). C.M. Quintão was funded by Fundação para a Ciência e Tecnologia (UID/FIS/04559/2013). S.S. Lopes participates in and acknowledge financial support from the COST Action BEAT-PCD (BM1407). S.S. Lopes received funding from project LysoCil funded by the European Union Horizon 2020 research and innovation under grant agreement No 811087. Funding information for this article has been deposited with the Crossref Funder Registry. |
publishDate |
2021 |
dc.date.none.fl_str_mv |
2021 2021-01-01T00:00:00Z 2022-10-17T22:11:48Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10362/144787 |
url |
http://hdl.handle.net/10362/144787 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
2312-0541 PURE: 47160234 https://doi.org/10.1183/23120541.00792-2020 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
12 application/pdf |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
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Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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RCAAP |
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RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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