Characterization of triacylglycerols in Madeira laurel oil by HPLC-atmospheric pressure chemical ionization-MS

• Autor(a) principal: Castilho, Paula C.
• Data de Publicação: 2004
• Outros Autores: Costa, Maria do Céu, Rodrigues, Ana, Branco, Pedro Costa, Costa, Mafalaa
• Tipo de documento: Artigo
• Idioma: eng
• Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
• Texto Completo: http://hdl.handle.net/10400.13/3271
• topic: APCI-MS; HPLC; Madeira laurel oil; Triacylglycerols; .; Faculdade de Ciências Exatas e da Engenharia

Madeira laurel oil was fractionated by liquid ex traction combined with TLC, and TAGs were analyzed by HPLC coupled with atmospheric pressure chemical ionization-MS (APCI-MS). Eluted molecular species compositions of the eluted TAG in the complex natural mixture were determined by GC identification of FAME and by LC-atmospheric pressure chemi cal ionization (APCI)-MS analysis of the lipid. The APCI-MS spectra of most TAG exhibited [M + H]+ and [M − RCOO]+ ions, which defined the M.W. and the molecular association of fatty acyl residues, respectively. Despite the relatively high de gree of saturation, with a saturated/unsaturated ratio of 0.70, no totally saturated TAG nor mixed asymmetric TAG with two sat urated FA (SSM or SSU, where S is saturated, M is monounsatu rated, and U is unsaturated) were found. This type of molecular structure provides a possible explanation for the relatively low m.p. (12–15°C) and also the high oxidative resistance observed.