Lupin protein concentrate as a novel functional food additive that can reduce colitis-induced inflammation and oxidative stress

Detalhes bibliográficos
Autor(a) principal: Mota, Joana
Data de Publicação: 2022
Outros Autores: Casimiro, Sandra, Fernandes, João, Hartmann, Renata M., Schemitt, Elizângela, Picada, Jaqueline, Costa, Luis, Marroni, Norma, Raymundo, Anabela, Lima, Ana, Ferreira, Ricardo Boavida
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10451/54615
Resumo: © 2022 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
id RCAP_e46d8f87c1f25e96031873caab12d260
oai_identifier_str oai:repositorio.ul.pt:10451/54615
network_acronym_str RCAP
network_name_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository_id_str 7160
spelling Lupin protein concentrate as a novel functional food additive that can reduce colitis-induced inflammation and oxidative stressHT29IBDMMP-9AntioxidantColitisCookiesFunctional foodLupin© 2022 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).Food fortification with bioactive compounds may constitute a way to ameliorate inflammatory bowel diseases (IBDs). Lupin seeds contain an oligomer named deflamin that can reduce IBD's symptoms via MMP-9 inhibition. Here, our goal was to develop a lupin protein concentrate (LPC) enriched in deflamin and to test its application as a food additive to be used as a functional food against colitis. The nutritional profile of the LPC was evaluated, and its efficacy in vivo was tested, either alone or as added to wheat cookies. The LPC presented high protein and carbohydrate contents (20.09 g/100 g and 62.05/100 g, respectively), as well as antioxidant activity (FRAP: 351.19 mg AAE/10 mg and DPPH: 273.9 mg AAE/10 mg). It was also effective against TNBS-induced colitis in a dose dependent-manner, reducing DAI scores by more than 50% and concomitantly inhibiting MMP-9 activity. When added to cookies, the LPC activities were maintained after baking, and a 4-day diet with LPC cookies induced a significant protective effect against acetic acid-induced colitis, overall bringing lesions, oxidative stress and DNA damage levels to values significantly similar to controls (p < 0.001). The results show that the LPC is an efficient way to deliver deflamin in IBD-targeted diets.This work was supported by national funds from Fundação para a Ciência e a Tecnologia (Lisbon, Portugal) through the research unit UID/AGR/04129/2020 (LEAF), project PTDC/BAA-AGR/28608/2017 and PhD grant to the first author (SFRH/BD/132832/2017).MDPIRepositório da Universidade de LisboaMota, JoanaCasimiro, SandraFernandes, JoãoHartmann, Renata M.Schemitt, ElizângelaPicada, JaquelineCosta, LuisMarroni, NormaRaymundo, AnabelaLima, AnaFerreira, Ricardo Boavida2022-09-28T13:44:24Z20222022-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10451/54615engNutrients. 2022 May 18;14(10):210210.3390/nu141021022072-6643info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-11-20T18:16:51Zoai:repositorio.ul.pt:10451/54615Portal AgregadorONGhttps://www.rcaap.pt/oai/openairemluisa.alvim@gmail.comopendoar:71602024-11-20T18:16:51Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Lupin protein concentrate as a novel functional food additive that can reduce colitis-induced inflammation and oxidative stress
title Lupin protein concentrate as a novel functional food additive that can reduce colitis-induced inflammation and oxidative stress
spellingShingle Lupin protein concentrate as a novel functional food additive that can reduce colitis-induced inflammation and oxidative stress
Mota, Joana
HT29
IBD
MMP-9
Antioxidant
Colitis
Cookies
Functional food
Lupin
title_short Lupin protein concentrate as a novel functional food additive that can reduce colitis-induced inflammation and oxidative stress
title_full Lupin protein concentrate as a novel functional food additive that can reduce colitis-induced inflammation and oxidative stress
title_fullStr Lupin protein concentrate as a novel functional food additive that can reduce colitis-induced inflammation and oxidative stress
title_full_unstemmed Lupin protein concentrate as a novel functional food additive that can reduce colitis-induced inflammation and oxidative stress
title_sort Lupin protein concentrate as a novel functional food additive that can reduce colitis-induced inflammation and oxidative stress
author Mota, Joana
author_facet Mota, Joana
Casimiro, Sandra
Fernandes, João
Hartmann, Renata M.
Schemitt, Elizângela
Picada, Jaqueline
Costa, Luis
Marroni, Norma
Raymundo, Anabela
Lima, Ana
Ferreira, Ricardo Boavida
author_role author
author2 Casimiro, Sandra
Fernandes, João
Hartmann, Renata M.
Schemitt, Elizângela
Picada, Jaqueline
Costa, Luis
Marroni, Norma
Raymundo, Anabela
Lima, Ana
Ferreira, Ricardo Boavida
author2_role author
author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Repositório da Universidade de Lisboa
dc.contributor.author.fl_str_mv Mota, Joana
Casimiro, Sandra
Fernandes, João
Hartmann, Renata M.
Schemitt, Elizângela
Picada, Jaqueline
Costa, Luis
Marroni, Norma
Raymundo, Anabela
Lima, Ana
Ferreira, Ricardo Boavida
dc.subject.por.fl_str_mv HT29
IBD
MMP-9
Antioxidant
Colitis
Cookies
Functional food
Lupin
topic HT29
IBD
MMP-9
Antioxidant
Colitis
Cookies
Functional food
Lupin
description © 2022 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
publishDate 2022
dc.date.none.fl_str_mv 2022-09-28T13:44:24Z
2022
2022-01-01T00:00:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10451/54615
url http://hdl.handle.net/10451/54615
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Nutrients. 2022 May 18;14(10):2102
10.3390/nu14102102
2072-6643
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv MDPI
publisher.none.fl_str_mv MDPI
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
repository.mail.fl_str_mv mluisa.alvim@gmail.com
_version_ 1817549204189347840

Registros relacionados