Cytotoxicity of Aspergillus section Fumigati isolated from health care environments

Detalhes bibliográficos
Autor(a) principal: Viegas, Carla
Data de Publicação: 2021
Outros Autores: Twarużek, Magdalena, Almeida, Beatriz, Dias, Marta, Ribeiro, Edna, Carolino, Elisabete, Soszczyńska, Ewelina, Caetano, Liliana Aranha
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10362/127188
Resumo: Funding Information: Funding: It was co-supported by FCT—Fundação para Ciência e Tecnologia for funding the project EXPOsE—Establishing protocols to assess occupational exposure to microbiota in clinical settings (02/SAICT/2016—Project no 23222), by the Instituto Politécnico de Lisboa, Lisbon, Portugal for funding the Project “Occupational exposure of ambulance drivers to bioburden” (IPL/2020/BIO-AmbuDrivers_ESTeSL) and by the Polish Minister of Science and Higher Education, under the program “Regional Initiative of Excellence” in 2019–2022 (Grant No. 008/RID/2018/19). Publisher Copyright: © 2021 by the authors. Licensee MDPI, Basel, Switzerland.
id RCAP_e4c9fa1c953e4df6e8c9b5dc98a460b7
oai_identifier_str oai:run.unl.pt:10362/127188
network_acronym_str RCAP
network_name_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository_id_str 7160
spelling Cytotoxicity of Aspergillus section Fumigati isolated from health care environmentsAspergillus section FumigatiCytotoxicityHealth care environmentLung epithelial cellsEcology, Evolution, Behavior and SystematicsPlant ScienceMicrobiology (medical)Funding Information: Funding: It was co-supported by FCT—Fundação para Ciência e Tecnologia for funding the project EXPOsE—Establishing protocols to assess occupational exposure to microbiota in clinical settings (02/SAICT/2016—Project no 23222), by the Instituto Politécnico de Lisboa, Lisbon, Portugal for funding the Project “Occupational exposure of ambulance drivers to bioburden” (IPL/2020/BIO-AmbuDrivers_ESTeSL) and by the Polish Minister of Science and Higher Education, under the program “Regional Initiative of Excellence” in 2019–2022 (Grant No. 008/RID/2018/19). Publisher Copyright: © 2021 by the authors. Licensee MDPI, Basel, Switzerland.This study analyzed 57 Aspergillus section Fumigati (AF) isolates collected by active and passive sampling (N = 450) in several health care facilities and from biological sampling of health care workers (N = 25) and controls (N = 22) in Portugal. All isolates were cultured in different me-dia and screened for azole resistance. Cytotoxicity was assessed for 40 isolates in lung epithelial cells and kidney cells using the MTT assay. Aspergillus section Fumigati was prevalent in the health care facilities and in nasal swabs from health care workers and controls. All AF isolates reduced cell viability and presented medium to high cytotoxicity, with cytotoxicity being significantly higher in A549 lung epithelial cells. The cytotoxicity of isolates from air and nasal swab samples suggested the inhalation route as a risk factor. Notably, 42% of AF isolates exhibited a pattern of reduced susceptibility to some of the most used antifungals available for the treatment of patients infected with these fungi. In sum, the epidemiology and clinical relevance of Aspergillus section Fumigati should continue to be addressed. A deeper understanding of the mechanisms underlying Aspergillus-mediated cytotoxicity is necessary.Comprehensive Health Research Centre (CHRC) - Pólo ENSPCentro de Investigação em Saúde Pública (CISP/PHRC)Escola Nacional de Saúde Pública (ENSP)RUNViegas, CarlaTwarużek, MagdalenaAlmeida, BeatrizDias, MartaRibeiro, EdnaCarolino, ElisabeteSoszczyńska, EwelinaCaetano, Liliana Aranha2021-11-04T23:38:42Z2021-102021-10-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10362/127188eng2309-608XPURE: 34680530https://doi.org/10.3390/jof7100839info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-03-11T05:07:16Zoai:run.unl.pt:10362/127188Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T03:46:04.177293Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Cytotoxicity of Aspergillus section Fumigati isolated from health care environments
title Cytotoxicity of Aspergillus section Fumigati isolated from health care environments
spellingShingle Cytotoxicity of Aspergillus section Fumigati isolated from health care environments
Viegas, Carla
Aspergillus section Fumigati
Cytotoxicity
Health care environment
Lung epithelial cells
Ecology, Evolution, Behavior and Systematics
Plant Science
Microbiology (medical)
title_short Cytotoxicity of Aspergillus section Fumigati isolated from health care environments
title_full Cytotoxicity of Aspergillus section Fumigati isolated from health care environments
title_fullStr Cytotoxicity of Aspergillus section Fumigati isolated from health care environments
title_full_unstemmed Cytotoxicity of Aspergillus section Fumigati isolated from health care environments
title_sort Cytotoxicity of Aspergillus section Fumigati isolated from health care environments
author Viegas, Carla
author_facet Viegas, Carla
Twarużek, Magdalena
Almeida, Beatriz
Dias, Marta
Ribeiro, Edna
Carolino, Elisabete
Soszczyńska, Ewelina
Caetano, Liliana Aranha
author_role author
author2 Twarużek, Magdalena
Almeida, Beatriz
Dias, Marta
Ribeiro, Edna
Carolino, Elisabete
Soszczyńska, Ewelina
Caetano, Liliana Aranha
author2_role author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Comprehensive Health Research Centre (CHRC) - Pólo ENSP
Centro de Investigação em Saúde Pública (CISP/PHRC)
Escola Nacional de Saúde Pública (ENSP)
RUN
dc.contributor.author.fl_str_mv Viegas, Carla
Twarużek, Magdalena
Almeida, Beatriz
Dias, Marta
Ribeiro, Edna
Carolino, Elisabete
Soszczyńska, Ewelina
Caetano, Liliana Aranha
dc.subject.por.fl_str_mv Aspergillus section Fumigati
Cytotoxicity
Health care environment
Lung epithelial cells
Ecology, Evolution, Behavior and Systematics
Plant Science
Microbiology (medical)
topic Aspergillus section Fumigati
Cytotoxicity
Health care environment
Lung epithelial cells
Ecology, Evolution, Behavior and Systematics
Plant Science
Microbiology (medical)
description Funding Information: Funding: It was co-supported by FCT—Fundação para Ciência e Tecnologia for funding the project EXPOsE—Establishing protocols to assess occupational exposure to microbiota in clinical settings (02/SAICT/2016—Project no 23222), by the Instituto Politécnico de Lisboa, Lisbon, Portugal for funding the Project “Occupational exposure of ambulance drivers to bioburden” (IPL/2020/BIO-AmbuDrivers_ESTeSL) and by the Polish Minister of Science and Higher Education, under the program “Regional Initiative of Excellence” in 2019–2022 (Grant No. 008/RID/2018/19). Publisher Copyright: © 2021 by the authors. Licensee MDPI, Basel, Switzerland.
publishDate 2021
dc.date.none.fl_str_mv 2021-11-04T23:38:42Z
2021-10
2021-10-01T00:00:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10362/127188
url http://hdl.handle.net/10362/127188
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 2309-608X
PURE: 34680530
https://doi.org/10.3390/jof7100839
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
repository.mail.fl_str_mv
_version_ 1799138064545611776

Registros relacionados