Cytotoxicity of Aspergillus section Fumigati isolated from health care environments
Autor(a) principal: | |
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Data de Publicação: | 2021 |
Outros Autores: | , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10362/127188 |
Resumo: | Funding Information: Funding: It was co-supported by FCT—Fundação para Ciência e Tecnologia for funding the project EXPOsE—Establishing protocols to assess occupational exposure to microbiota in clinical settings (02/SAICT/2016—Project no 23222), by the Instituto Politécnico de Lisboa, Lisbon, Portugal for funding the Project “Occupational exposure of ambulance drivers to bioburden” (IPL/2020/BIO-AmbuDrivers_ESTeSL) and by the Polish Minister of Science and Higher Education, under the program “Regional Initiative of Excellence” in 2019–2022 (Grant No. 008/RID/2018/19). Publisher Copyright: © 2021 by the authors. Licensee MDPI, Basel, Switzerland. |
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Cytotoxicity of Aspergillus section Fumigati isolated from health care environmentsAspergillus section FumigatiCytotoxicityHealth care environmentLung epithelial cellsEcology, Evolution, Behavior and SystematicsPlant ScienceMicrobiology (medical)Funding Information: Funding: It was co-supported by FCT—Fundação para Ciência e Tecnologia for funding the project EXPOsE—Establishing protocols to assess occupational exposure to microbiota in clinical settings (02/SAICT/2016—Project no 23222), by the Instituto Politécnico de Lisboa, Lisbon, Portugal for funding the Project “Occupational exposure of ambulance drivers to bioburden” (IPL/2020/BIO-AmbuDrivers_ESTeSL) and by the Polish Minister of Science and Higher Education, under the program “Regional Initiative of Excellence” in 2019–2022 (Grant No. 008/RID/2018/19). Publisher Copyright: © 2021 by the authors. Licensee MDPI, Basel, Switzerland.This study analyzed 57 Aspergillus section Fumigati (AF) isolates collected by active and passive sampling (N = 450) in several health care facilities and from biological sampling of health care workers (N = 25) and controls (N = 22) in Portugal. All isolates were cultured in different me-dia and screened for azole resistance. Cytotoxicity was assessed for 40 isolates in lung epithelial cells and kidney cells using the MTT assay. Aspergillus section Fumigati was prevalent in the health care facilities and in nasal swabs from health care workers and controls. All AF isolates reduced cell viability and presented medium to high cytotoxicity, with cytotoxicity being significantly higher in A549 lung epithelial cells. The cytotoxicity of isolates from air and nasal swab samples suggested the inhalation route as a risk factor. Notably, 42% of AF isolates exhibited a pattern of reduced susceptibility to some of the most used antifungals available for the treatment of patients infected with these fungi. In sum, the epidemiology and clinical relevance of Aspergillus section Fumigati should continue to be addressed. A deeper understanding of the mechanisms underlying Aspergillus-mediated cytotoxicity is necessary.Comprehensive Health Research Centre (CHRC) - Pólo ENSPCentro de Investigação em Saúde Pública (CISP/PHRC)Escola Nacional de Saúde Pública (ENSP)RUNViegas, CarlaTwarużek, MagdalenaAlmeida, BeatrizDias, MartaRibeiro, EdnaCarolino, ElisabeteSoszczyńska, EwelinaCaetano, Liliana Aranha2021-11-04T23:38:42Z2021-102021-10-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10362/127188eng2309-608XPURE: 34680530https://doi.org/10.3390/jof7100839info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-03-11T05:07:16Zoai:run.unl.pt:10362/127188Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T03:46:04.177293Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Cytotoxicity of Aspergillus section Fumigati isolated from health care environments |
title |
Cytotoxicity of Aspergillus section Fumigati isolated from health care environments |
spellingShingle |
Cytotoxicity of Aspergillus section Fumigati isolated from health care environments Viegas, Carla Aspergillus section Fumigati Cytotoxicity Health care environment Lung epithelial cells Ecology, Evolution, Behavior and Systematics Plant Science Microbiology (medical) |
title_short |
Cytotoxicity of Aspergillus section Fumigati isolated from health care environments |
title_full |
Cytotoxicity of Aspergillus section Fumigati isolated from health care environments |
title_fullStr |
Cytotoxicity of Aspergillus section Fumigati isolated from health care environments |
title_full_unstemmed |
Cytotoxicity of Aspergillus section Fumigati isolated from health care environments |
title_sort |
Cytotoxicity of Aspergillus section Fumigati isolated from health care environments |
author |
Viegas, Carla |
author_facet |
Viegas, Carla Twarużek, Magdalena Almeida, Beatriz Dias, Marta Ribeiro, Edna Carolino, Elisabete Soszczyńska, Ewelina Caetano, Liliana Aranha |
author_role |
author |
author2 |
Twarużek, Magdalena Almeida, Beatriz Dias, Marta Ribeiro, Edna Carolino, Elisabete Soszczyńska, Ewelina Caetano, Liliana Aranha |
author2_role |
author author author author author author author |
dc.contributor.none.fl_str_mv |
Comprehensive Health Research Centre (CHRC) - Pólo ENSP Centro de Investigação em Saúde Pública (CISP/PHRC) Escola Nacional de Saúde Pública (ENSP) RUN |
dc.contributor.author.fl_str_mv |
Viegas, Carla Twarużek, Magdalena Almeida, Beatriz Dias, Marta Ribeiro, Edna Carolino, Elisabete Soszczyńska, Ewelina Caetano, Liliana Aranha |
dc.subject.por.fl_str_mv |
Aspergillus section Fumigati Cytotoxicity Health care environment Lung epithelial cells Ecology, Evolution, Behavior and Systematics Plant Science Microbiology (medical) |
topic |
Aspergillus section Fumigati Cytotoxicity Health care environment Lung epithelial cells Ecology, Evolution, Behavior and Systematics Plant Science Microbiology (medical) |
description |
Funding Information: Funding: It was co-supported by FCT—Fundação para Ciência e Tecnologia for funding the project EXPOsE—Establishing protocols to assess occupational exposure to microbiota in clinical settings (02/SAICT/2016—Project no 23222), by the Instituto Politécnico de Lisboa, Lisbon, Portugal for funding the Project “Occupational exposure of ambulance drivers to bioburden” (IPL/2020/BIO-AmbuDrivers_ESTeSL) and by the Polish Minister of Science and Higher Education, under the program “Regional Initiative of Excellence” in 2019–2022 (Grant No. 008/RID/2018/19). Publisher Copyright: © 2021 by the authors. Licensee MDPI, Basel, Switzerland. |
publishDate |
2021 |
dc.date.none.fl_str_mv |
2021-11-04T23:38:42Z 2021-10 2021-10-01T00:00:00Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10362/127188 |
url |
http://hdl.handle.net/10362/127188 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
2309-608X PURE: 34680530 https://doi.org/10.3390/jof7100839 |
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info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
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application/pdf |
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reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
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Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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RCAAP |
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RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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