Pervasive transcription read-through promotes aberrant expression of oncogenes and RNA chimeras in renal carcinoma

Detalhes bibliográficos
Autor(a) principal: Grosso, Ana Rita
Data de Publicação: 2015
Outros Autores: Leite, Ana Paula, Carvalho, Silvia, Ramos De Matos, Mafalda, Martins, Filipa B., Vítor, Alexandra, Desterro, Joana, Carmo-Fonseca, Maria, de Almeida, Sérgio F.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10451/51125
Resumo: © 2015, Grosso et al. This article is distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use and redistribution provided that the original author and source are credited.
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spelling Pervasive transcription read-through promotes aberrant expression of oncogenes and RNA chimeras in renal carcinomaBCL2 oncogeneRNA chimerasCancer genesChromosomesClear cell renal cell carcinomaGenesHumanHuman biologyMedicineTranscription read-through© 2015, Grosso et al. This article is distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use and redistribution provided that the original author and source are credited.Aberrant expression of cancer genes and non-canonical RNA species is a hallmark of cancer. However, the mechanisms driving such atypical gene expression programs are incompletely understood. Here, our transcriptional profiling of a cohort of 50 primary clear cell renal cell carcinoma (ccRCC) samples from The Cancer Genome Atlas (TCGA) reveals that transcription read-through beyond the termination site is a source of transcriptome diversity in cancer cells. Amongst the genes most frequently mutated in ccRCC, we identified SETD2 inactivation as a potent enhancer of transcription read-through. We further show that invasion of neighbouring genes and generation of RNA chimeras are functional outcomes of transcription read-through. We identified the BCL2 oncogene as one of such invaded genes and detected a novel chimera, the CTSC-RAB38, in 20% of ccRCC samples. Collectively, our data highlight a novel link between transcription read-through and aberrant expression of oncogenes and chimeric transcripts that is prevalent in cancer.This work was supported by Fundação para a Ciência e Tecnologia (FCT), Portugal (PTDC/BIM-ONC/0384-2012 to SFdA). MRM is a FCT PhD fellow (SFRH/BD/92208/2013). ACV is a Lisbon BioMed PhD fellow funded by FCT (SFRH/BD/52232/2013). ARG is the recipient of a FCT Investigator award (IF/00510/2014).eLifeRepositório da Universidade de LisboaGrosso, Ana RitaLeite, Ana PaulaCarvalho, SilviaRamos De Matos, MafaldaMartins, Filipa B.Vítor, AlexandraDesterro, JoanaCarmo-Fonseca, Mariade Almeida, Sérgio F.2022-02-04T15:21:41Z20152015-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10451/51125engElife. 2015 Nov 17;4:e09214.10.7554/eLife.092142050-084Xinfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-11-08T16:55:38Zoai:repositorio.ul.pt:10451/51125Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T22:02:26.673666Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Pervasive transcription read-through promotes aberrant expression of oncogenes and RNA chimeras in renal carcinoma
title Pervasive transcription read-through promotes aberrant expression of oncogenes and RNA chimeras in renal carcinoma
spellingShingle Pervasive transcription read-through promotes aberrant expression of oncogenes and RNA chimeras in renal carcinoma
Grosso, Ana Rita
BCL2 oncogene
RNA chimeras
Cancer genes
Chromosomes
Clear cell renal cell carcinoma
Genes
Human
Human biology
Medicine
Transcription read-through
title_short Pervasive transcription read-through promotes aberrant expression of oncogenes and RNA chimeras in renal carcinoma
title_full Pervasive transcription read-through promotes aberrant expression of oncogenes and RNA chimeras in renal carcinoma
title_fullStr Pervasive transcription read-through promotes aberrant expression of oncogenes and RNA chimeras in renal carcinoma
title_full_unstemmed Pervasive transcription read-through promotes aberrant expression of oncogenes and RNA chimeras in renal carcinoma
title_sort Pervasive transcription read-through promotes aberrant expression of oncogenes and RNA chimeras in renal carcinoma
author Grosso, Ana Rita
author_facet Grosso, Ana Rita
Leite, Ana Paula
Carvalho, Silvia
Ramos De Matos, Mafalda
Martins, Filipa B.
Vítor, Alexandra
Desterro, Joana
Carmo-Fonseca, Maria
de Almeida, Sérgio F.
author_role author
author2 Leite, Ana Paula
Carvalho, Silvia
Ramos De Matos, Mafalda
Martins, Filipa B.
Vítor, Alexandra
Desterro, Joana
Carmo-Fonseca, Maria
de Almeida, Sérgio F.
author2_role author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Repositório da Universidade de Lisboa
dc.contributor.author.fl_str_mv Grosso, Ana Rita
Leite, Ana Paula
Carvalho, Silvia
Ramos De Matos, Mafalda
Martins, Filipa B.
Vítor, Alexandra
Desterro, Joana
Carmo-Fonseca, Maria
de Almeida, Sérgio F.
dc.subject.por.fl_str_mv BCL2 oncogene
RNA chimeras
Cancer genes
Chromosomes
Clear cell renal cell carcinoma
Genes
Human
Human biology
Medicine
Transcription read-through
topic BCL2 oncogene
RNA chimeras
Cancer genes
Chromosomes
Clear cell renal cell carcinoma
Genes
Human
Human biology
Medicine
Transcription read-through
description © 2015, Grosso et al. This article is distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use and redistribution provided that the original author and source are credited.
publishDate 2015
dc.date.none.fl_str_mv 2015
2015-01-01T00:00:00Z
2022-02-04T15:21:41Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10451/51125
url http://hdl.handle.net/10451/51125
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Elife. 2015 Nov 17;4:e09214.
10.7554/eLife.09214
2050-084X
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv eLife
publisher.none.fl_str_mv eLife
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
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reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
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