Substituted borosilicate glasses with improved osteogenic capacity for bone tissue engineering
Autor(a) principal: | |
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Data de Publicação: | 2017 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/1822/46996 |
Resumo: | Borosilicate bioactive glasses (BBGs) have shown the capacity to promote higher formation of new bone when compared with silicate bioactive glasses. Herein, we assessed the capacity of BBGs to induce osteogenic differentiation of bone marrow mesenchymal stem cells (BM-MSCs) as a function of their substituted divalent cations (Mg2+, Ca2+, Sr2+). To this purpose, we synthesized BBG particles by melt quenching. The cell viability, proliferation, and morphology (i.e., PrestoBlue®, PicoGreen®, and DAPI and Phalloidin stainings, respectively), as well as protein expression (i.e., alkaline phosphatase, ALP; osteopontin, OP; and osteocalcin, OC), of BM-MSCs in contact with BBGs were evaluated for 21 days. We observed an enhanced expression of bone-specific proteins (ALP, OP, and OC) and high mineralization of BM-MSCs under BBG-Mg and BBG-Sr-conditioned osteogenic media for concentrations of 20 and 50 mg/mL with low cytotoxic effects. Moreover, BBG-Sr, at a concentration of 50 mg/mL, was able to increase the mineralization and expression of the same bone-specific proteins even under basal medium conditions. These results indicated that the proposed BBGs improved osteogenic differentiation of BM-MSCs, therefore showing their potential as relevant biomaterials for bone tissue regeneration, not only by bonding to bone tissue but also by stimulating new bone formation. |
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Substituted borosilicate glasses with improved osteogenic capacity for bone tissue engineeringBM-MSCsBorosilicate glassesMineralisationOsteogenic inductionStrontiummineralizationNineralizationScience & TechnologyBorosilicate bioactive glasses (BBGs) have shown the capacity to promote higher formation of new bone when compared with silicate bioactive glasses. Herein, we assessed the capacity of BBGs to induce osteogenic differentiation of bone marrow mesenchymal stem cells (BM-MSCs) as a function of their substituted divalent cations (Mg2+, Ca2+, Sr2+). To this purpose, we synthesized BBG particles by melt quenching. The cell viability, proliferation, and morphology (i.e., PrestoBlue®, PicoGreen®, and DAPI and Phalloidin stainings, respectively), as well as protein expression (i.e., alkaline phosphatase, ALP; osteopontin, OP; and osteocalcin, OC), of BM-MSCs in contact with BBGs were evaluated for 21 days. We observed an enhanced expression of bone-specific proteins (ALP, OP, and OC) and high mineralization of BM-MSCs under BBG-Mg and BBG-Sr-conditioned osteogenic media for concentrations of 20 and 50 mg/mL with low cytotoxic effects. Moreover, BBG-Sr, at a concentration of 50 mg/mL, was able to increase the mineralization and expression of the same bone-specific proteins even under basal medium conditions. These results indicated that the proposed BBGs improved osteogenic differentiation of BM-MSCs, therefore showing their potential as relevant biomaterials for bone tissue regeneration, not only by bonding to bone tissue but also by stimulating new bone formation.The authors gratefully acknowledge financial support from Portuguese Foundation for Science and Technology (PhD grant BD/73162/2010), the European Commission Seventh Framework Programme (FP7/2007-2013) under Grant No. REGPOT-CT2012-31633-POLARIS, and Horizon 2020 Programme under Grant No. WIDESPREAD-2014-2-668983-FORECAST. This work was also supported by the European Research Council grant agreement ERC-2012-ADG-20120216-321266 for the project ComplexiTE and is associated with the UK EPSRC Centre for Innovative Manufacturing of Medical Devices-MeDe Innovation (EPSRC grant EP/K029592/1) where Fernandes was a visiting researcher.info:eu-repo/semantics/publishedVersionMary Ann Liebert Inc.Universidade do MinhoFernandes, João S.Gentile, PiergiorgioCrawford, AileenPires, R. A.Hatton, Paul V.Reis, R. L.20172017-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/1822/46996engFernandes J. S., Gentile P., Crawford A., Pires R. A., Hatton P. V., Reis R. L. Substituted Borosilicate Glasses with Improved Osteogenic Capacity for Bone Tissue Engineering, Tissue Engineering, doi:10.1089/ten.TEA.2016.0386, 2017.2152-495510.1089/ten.tea.2016.038628346797info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-21T12:41:56Zoai:repositorium.sdum.uminho.pt:1822/46996Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T19:39:02.031087Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Substituted borosilicate glasses with improved osteogenic capacity for bone tissue engineering |
title |
Substituted borosilicate glasses with improved osteogenic capacity for bone tissue engineering |
spellingShingle |
Substituted borosilicate glasses with improved osteogenic capacity for bone tissue engineering Fernandes, João S. BM-MSCs Borosilicate glasses Mineralisation Osteogenic induction Strontium mineralization Nineralization Science & Technology |
title_short |
Substituted borosilicate glasses with improved osteogenic capacity for bone tissue engineering |
title_full |
Substituted borosilicate glasses with improved osteogenic capacity for bone tissue engineering |
title_fullStr |
Substituted borosilicate glasses with improved osteogenic capacity for bone tissue engineering |
title_full_unstemmed |
Substituted borosilicate glasses with improved osteogenic capacity for bone tissue engineering |
title_sort |
Substituted borosilicate glasses with improved osteogenic capacity for bone tissue engineering |
author |
Fernandes, João S. |
author_facet |
Fernandes, João S. Gentile, Piergiorgio Crawford, Aileen Pires, R. A. Hatton, Paul V. Reis, R. L. |
author_role |
author |
author2 |
Gentile, Piergiorgio Crawford, Aileen Pires, R. A. Hatton, Paul V. Reis, R. L. |
author2_role |
author author author author author |
dc.contributor.none.fl_str_mv |
Universidade do Minho |
dc.contributor.author.fl_str_mv |
Fernandes, João S. Gentile, Piergiorgio Crawford, Aileen Pires, R. A. Hatton, Paul V. Reis, R. L. |
dc.subject.por.fl_str_mv |
BM-MSCs Borosilicate glasses Mineralisation Osteogenic induction Strontium mineralization Nineralization Science & Technology |
topic |
BM-MSCs Borosilicate glasses Mineralisation Osteogenic induction Strontium mineralization Nineralization Science & Technology |
description |
Borosilicate bioactive glasses (BBGs) have shown the capacity to promote higher formation of new bone when compared with silicate bioactive glasses. Herein, we assessed the capacity of BBGs to induce osteogenic differentiation of bone marrow mesenchymal stem cells (BM-MSCs) as a function of their substituted divalent cations (Mg2+, Ca2+, Sr2+). To this purpose, we synthesized BBG particles by melt quenching. The cell viability, proliferation, and morphology (i.e., PrestoBlue®, PicoGreen®, and DAPI and Phalloidin stainings, respectively), as well as protein expression (i.e., alkaline phosphatase, ALP; osteopontin, OP; and osteocalcin, OC), of BM-MSCs in contact with BBGs were evaluated for 21 days. We observed an enhanced expression of bone-specific proteins (ALP, OP, and OC) and high mineralization of BM-MSCs under BBG-Mg and BBG-Sr-conditioned osteogenic media for concentrations of 20 and 50 mg/mL with low cytotoxic effects. Moreover, BBG-Sr, at a concentration of 50 mg/mL, was able to increase the mineralization and expression of the same bone-specific proteins even under basal medium conditions. These results indicated that the proposed BBGs improved osteogenic differentiation of BM-MSCs, therefore showing their potential as relevant biomaterials for bone tissue regeneration, not only by bonding to bone tissue but also by stimulating new bone formation. |
publishDate |
2017 |
dc.date.none.fl_str_mv |
2017 2017-01-01T00:00:00Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/1822/46996 |
url |
http://hdl.handle.net/1822/46996 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Fernandes J. S., Gentile P., Crawford A., Pires R. A., Hatton P. V., Reis R. L. Substituted Borosilicate Glasses with Improved Osteogenic Capacity for Bone Tissue Engineering, Tissue Engineering, doi:10.1089/ten.TEA.2016.0386, 2017. 2152-4955 10.1089/ten.tea.2016.0386 28346797 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Mary Ann Liebert Inc. |
publisher.none.fl_str_mv |
Mary Ann Liebert Inc. |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
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Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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