Investigation of urinary volatomic alterations in head and neck cancer: a non-invasive approach towards diagnosis and prognosis
Autor(a) principal: | |
---|---|
Data de Publicação: | 2017 |
Outros Autores: | , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10400.13/3878 |
Resumo: | Introduction Head and neck cancer (HNC), like many other forms of cancer, is usually detected in advanced stages, causing poor survival outcomes. Lack of specific and sensi tive screening markers for early detection of HNC has wors ened the scenario for the patients as well as the clinicians. Therefore, identification of efficient, noninvasive and afford able screening marker/methodology with high specificity and sensitivity is imminent need of situation. Objectives This study aims to identify and characterize urinary volatomic alterations specific to HNC. Methods Volatomic analysis of urine samples collected from HNC patients (n=29) and healthy controls (n=31) was performed using headspace solid phase microextraction coupled to gas chromatography mass spectrometry (GC– MS). Both univariate and multivariate statistical approaches were used to investigate HNC specific volatomic alterations. Results Statistical analysis revealed a total of 28 metabo lites with highest contribution towards discrimination of HNC patients from healthy controls (VIP >1, p < 0.05, Log2 FC ≥0.58/≤−0.57). The discrimination efficiency and accuracy of urinary VOCs was ascertained by ROC curve analysis that allowed the identification of four metabolites viz. 2,6-dimethyl-7-octen-2-ol, 1-butanol, p-xylene and 4-methyl-2-heptanone with highest sensitivity and speci ficity to discriminate HNC patients from healthy controls. Further, the metabolic pathway analysis identified several dysregulated pathways in HNC patients and their detailed investigations could unravel novel mechanistic insights into the disease pathophysiology. Conclusion Overall, this study provides valuable finger print of the volatile profile of HNC patients, which in turn, might help in improving the current understanding of this form of cancer and lead to the development of non-invasive approaches for HNC diagnosis. |
id |
RCAP_e9293be77791a5e8d23d02d993ca5b43 |
---|---|
oai_identifier_str |
oai:digituma.uma.pt:10400.13/3878 |
network_acronym_str |
RCAP |
network_name_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository_id_str |
7160 |
spelling |
Investigation of urinary volatomic alterations in head and neck cancer: a non-invasive approach towards diagnosis and prognosisHead and neck cancerVolatomicsVOMsGC–MSUrinary markers.Faculdade de Ciências Exatas e da EngenhariaCentro de Química da MadeiraIntroduction Head and neck cancer (HNC), like many other forms of cancer, is usually detected in advanced stages, causing poor survival outcomes. Lack of specific and sensi tive screening markers for early detection of HNC has wors ened the scenario for the patients as well as the clinicians. Therefore, identification of efficient, noninvasive and afford able screening marker/methodology with high specificity and sensitivity is imminent need of situation. Objectives This study aims to identify and characterize urinary volatomic alterations specific to HNC. Methods Volatomic analysis of urine samples collected from HNC patients (n=29) and healthy controls (n=31) was performed using headspace solid phase microextraction coupled to gas chromatography mass spectrometry (GC– MS). Both univariate and multivariate statistical approaches were used to investigate HNC specific volatomic alterations. Results Statistical analysis revealed a total of 28 metabo lites with highest contribution towards discrimination of HNC patients from healthy controls (VIP >1, p < 0.05, Log2 FC ≥0.58/≤−0.57). The discrimination efficiency and accuracy of urinary VOCs was ascertained by ROC curve analysis that allowed the identification of four metabolites viz. 2,6-dimethyl-7-octen-2-ol, 1-butanol, p-xylene and 4-methyl-2-heptanone with highest sensitivity and speci ficity to discriminate HNC patients from healthy controls. Further, the metabolic pathway analysis identified several dysregulated pathways in HNC patients and their detailed investigations could unravel novel mechanistic insights into the disease pathophysiology. Conclusion Overall, this study provides valuable finger print of the volatile profile of HNC patients, which in turn, might help in improving the current understanding of this form of cancer and lead to the development of non-invasive approaches for HNC diagnosis.SpringerDigitUMaTaware, RavindraTaunk, KhushmanPereira, Jorge A. M.Dhakne, RahulKannan, NarayananSoneji, DharmeshCâmara, José S.Nagarajaram, H. A.Rapole, Srikanth2021-12-02T12:05:21Z20172017-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10400.13/3878engTaware, R., Taunk, K., Pereira, J. A., Dhakne, R., Kannan, N., Soneji, D., ... & Rapole, S. (2017). Investigation of urinary volatomic alterations in head and neck cancer: a non-invasive approach towards diagnosis and prognosis. Metabolomics, 13(10), 1-11. DOI 10.1007/s11306-017-1251-610.1007/s11306-017-1251-6info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-03-17T05:57:53Zoai:digituma.uma.pt:10400.13/3878Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T15:07:27.090350Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Investigation of urinary volatomic alterations in head and neck cancer: a non-invasive approach towards diagnosis and prognosis |
title |
Investigation of urinary volatomic alterations in head and neck cancer: a non-invasive approach towards diagnosis and prognosis |
spellingShingle |
Investigation of urinary volatomic alterations in head and neck cancer: a non-invasive approach towards diagnosis and prognosis Taware, Ravindra Head and neck cancer Volatomics VOMs GC–MS Urinary markers . Faculdade de Ciências Exatas e da Engenharia Centro de Química da Madeira |
title_short |
Investigation of urinary volatomic alterations in head and neck cancer: a non-invasive approach towards diagnosis and prognosis |
title_full |
Investigation of urinary volatomic alterations in head and neck cancer: a non-invasive approach towards diagnosis and prognosis |
title_fullStr |
Investigation of urinary volatomic alterations in head and neck cancer: a non-invasive approach towards diagnosis and prognosis |
title_full_unstemmed |
Investigation of urinary volatomic alterations in head and neck cancer: a non-invasive approach towards diagnosis and prognosis |
title_sort |
Investigation of urinary volatomic alterations in head and neck cancer: a non-invasive approach towards diagnosis and prognosis |
author |
Taware, Ravindra |
author_facet |
Taware, Ravindra Taunk, Khushman Pereira, Jorge A. M. Dhakne, Rahul Kannan, Narayanan Soneji, Dharmesh Câmara, José S. Nagarajaram, H. A. Rapole, Srikanth |
author_role |
author |
author2 |
Taunk, Khushman Pereira, Jorge A. M. Dhakne, Rahul Kannan, Narayanan Soneji, Dharmesh Câmara, José S. Nagarajaram, H. A. Rapole, Srikanth |
author2_role |
author author author author author author author author |
dc.contributor.none.fl_str_mv |
DigitUMa |
dc.contributor.author.fl_str_mv |
Taware, Ravindra Taunk, Khushman Pereira, Jorge A. M. Dhakne, Rahul Kannan, Narayanan Soneji, Dharmesh Câmara, José S. Nagarajaram, H. A. Rapole, Srikanth |
dc.subject.por.fl_str_mv |
Head and neck cancer Volatomics VOMs GC–MS Urinary markers . Faculdade de Ciências Exatas e da Engenharia Centro de Química da Madeira |
topic |
Head and neck cancer Volatomics VOMs GC–MS Urinary markers . Faculdade de Ciências Exatas e da Engenharia Centro de Química da Madeira |
description |
Introduction Head and neck cancer (HNC), like many other forms of cancer, is usually detected in advanced stages, causing poor survival outcomes. Lack of specific and sensi tive screening markers for early detection of HNC has wors ened the scenario for the patients as well as the clinicians. Therefore, identification of efficient, noninvasive and afford able screening marker/methodology with high specificity and sensitivity is imminent need of situation. Objectives This study aims to identify and characterize urinary volatomic alterations specific to HNC. Methods Volatomic analysis of urine samples collected from HNC patients (n=29) and healthy controls (n=31) was performed using headspace solid phase microextraction coupled to gas chromatography mass spectrometry (GC– MS). Both univariate and multivariate statistical approaches were used to investigate HNC specific volatomic alterations. Results Statistical analysis revealed a total of 28 metabo lites with highest contribution towards discrimination of HNC patients from healthy controls (VIP >1, p < 0.05, Log2 FC ≥0.58/≤−0.57). The discrimination efficiency and accuracy of urinary VOCs was ascertained by ROC curve analysis that allowed the identification of four metabolites viz. 2,6-dimethyl-7-octen-2-ol, 1-butanol, p-xylene and 4-methyl-2-heptanone with highest sensitivity and speci ficity to discriminate HNC patients from healthy controls. Further, the metabolic pathway analysis identified several dysregulated pathways in HNC patients and their detailed investigations could unravel novel mechanistic insights into the disease pathophysiology. Conclusion Overall, this study provides valuable finger print of the volatile profile of HNC patients, which in turn, might help in improving the current understanding of this form of cancer and lead to the development of non-invasive approaches for HNC diagnosis. |
publishDate |
2017 |
dc.date.none.fl_str_mv |
2017 2017-01-01T00:00:00Z 2021-12-02T12:05:21Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10400.13/3878 |
url |
http://hdl.handle.net/10400.13/3878 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Taware, R., Taunk, K., Pereira, J. A., Dhakne, R., Kannan, N., Soneji, D., ... & Rapole, S. (2017). Investigation of urinary volatomic alterations in head and neck cancer: a non-invasive approach towards diagnosis and prognosis. Metabolomics, 13(10), 1-11. DOI 10.1007/s11306-017-1251-6 10.1007/s11306-017-1251-6 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Springer |
publisher.none.fl_str_mv |
Springer |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
instname_str |
Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
instacron_str |
RCAAP |
institution |
RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
collection |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository.name.fl_str_mv |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
repository.mail.fl_str_mv |
|
_version_ |
1799129943657938944 |