Assessing the antibiotic susceptibility of freshwater Cyanobacteria spp.
Autor(a) principal: | |
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Data de Publicação: | 2015 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10400.18/3557 |
Resumo: | Freshwater is a vehicle for the emergence and dissemination of Antibiotic resistance. Cyanobacteria are ubiquitous in freshwater, where they are exposed to antibiotics and resistant organisms, but their role on water resistome was never evaluated. Data concerning the effects of antibiotics on cyanobacteria, obtained by distinct methodologies, is often contradictory. This emphasizes the importance of developing procedures to understand the trends of antibiotic susceptibility in cyanobacteria. In this study we aimed to evaluate the susceptibility of four cyanobacterial isolates from different genera (Microcystis aeruginosa, Aphanizomenon gracile, Chrisosporum bergii, Planktothix agradhii), and among them nine isolates from the same specie (M.aeruginosa) to distinct antibiotics (amoxicillin, ceftazidime, ceftriaxone, kanamycine, gentamicine, tetracycline, trimethoprim, nalidixicacid, norfloxacin). We used a method adapted from the bacteria standard broth microdilution. Cyanobacteria were exposed to serial dilution of each antibiotic (0.0015–1.6mg/L) in Z8 medium (20±1◦C; 14/10hL/D cycle; light intensity 16±421μEm−s−). Cell growth was followed overtime (OD450nm/microscopic examination) and the minimum inhibitory concentrations (MICs) were calculated for each antibiotic/ isolate. We found that β-lactams exhibited the lower MICs, aminoglycosides, tetracycline and norfloxacine presented intermediate MICs; none of the isolates were susceptible to trimethoprim and nalidixic acid. The reduced susceptibility of all tested cyanobacteria to some antibiotics suggests that they might be naturally non-susceptible to these compounds, or that they might became non-susceptible due to antibiotic contamination pressure, or to the transfer of genes from resistant bacteria present in the environment. |
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Assessing the antibiotic susceptibility of freshwater Cyanobacteria spp.CyanobacteriaAntibioticsMinimum Inhibitory ConcentrationsReduced SusceptibilityWaterResistência aos AntimicrobianosÁgua e SoloFreshwater is a vehicle for the emergence and dissemination of Antibiotic resistance. Cyanobacteria are ubiquitous in freshwater, where they are exposed to antibiotics and resistant organisms, but their role on water resistome was never evaluated. Data concerning the effects of antibiotics on cyanobacteria, obtained by distinct methodologies, is often contradictory. This emphasizes the importance of developing procedures to understand the trends of antibiotic susceptibility in cyanobacteria. In this study we aimed to evaluate the susceptibility of four cyanobacterial isolates from different genera (Microcystis aeruginosa, Aphanizomenon gracile, Chrisosporum bergii, Planktothix agradhii), and among them nine isolates from the same specie (M.aeruginosa) to distinct antibiotics (amoxicillin, ceftazidime, ceftriaxone, kanamycine, gentamicine, tetracycline, trimethoprim, nalidixicacid, norfloxacin). We used a method adapted from the bacteria standard broth microdilution. Cyanobacteria were exposed to serial dilution of each antibiotic (0.0015–1.6mg/L) in Z8 medium (20±1◦C; 14/10hL/D cycle; light intensity 16±421μEm−s−). Cell growth was followed overtime (OD450nm/microscopic examination) and the minimum inhibitory concentrations (MICs) were calculated for each antibiotic/ isolate. We found that β-lactams exhibited the lower MICs, aminoglycosides, tetracycline and norfloxacine presented intermediate MICs; none of the isolates were susceptible to trimethoprim and nalidixic acid. The reduced susceptibility of all tested cyanobacteria to some antibiotics suggests that they might be naturally non-susceptible to these compounds, or that they might became non-susceptible due to antibiotic contamination pressure, or to the transfer of genes from resistant bacteria present in the environment.ED, VM, and DJ have received research funding from Fundação para a Ciência e Tecnologia via grants SFRH/BPD/77981/2011, SFRH/BPD/77486/2011, and SFRH/BD/80001/2011, respectively. The authors thank Fundação para a Ciência e a Tecnologia (FCT) for project grant PEst-OE/AGR/UI0211/2011-2014, Strategic Project UI211-2011-2014.Frontiers MediaRepositório Científico do Instituto Nacional de SaúdeDias, ElsaOliveira, MicaelaJones-Dias, DanielaVasconcelos, VitorFerreira, EugéniaManageiro, VeraCaniça, Manuela2016-03-01T17:04:02Z2015-08-112015-08-11T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10400.18/3557engFront Microbiol. 2015 Aug 11;6:799. doi: 10.3389/fmicb.2015.00799. eCollection 20151664-302X10.3389/fmicb.2015.00799info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-20T15:39:51Zoai:repositorio.insa.pt:10400.18/3557Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T18:38:25.302752Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Assessing the antibiotic susceptibility of freshwater Cyanobacteria spp. |
title |
Assessing the antibiotic susceptibility of freshwater Cyanobacteria spp. |
spellingShingle |
Assessing the antibiotic susceptibility of freshwater Cyanobacteria spp. Dias, Elsa Cyanobacteria Antibiotics Minimum Inhibitory Concentrations Reduced Susceptibility Water Resistência aos Antimicrobianos Água e Solo |
title_short |
Assessing the antibiotic susceptibility of freshwater Cyanobacteria spp. |
title_full |
Assessing the antibiotic susceptibility of freshwater Cyanobacteria spp. |
title_fullStr |
Assessing the antibiotic susceptibility of freshwater Cyanobacteria spp. |
title_full_unstemmed |
Assessing the antibiotic susceptibility of freshwater Cyanobacteria spp. |
title_sort |
Assessing the antibiotic susceptibility of freshwater Cyanobacteria spp. |
author |
Dias, Elsa |
author_facet |
Dias, Elsa Oliveira, Micaela Jones-Dias, Daniela Vasconcelos, Vitor Ferreira, Eugénia Manageiro, Vera Caniça, Manuela |
author_role |
author |
author2 |
Oliveira, Micaela Jones-Dias, Daniela Vasconcelos, Vitor Ferreira, Eugénia Manageiro, Vera Caniça, Manuela |
author2_role |
author author author author author author |
dc.contributor.none.fl_str_mv |
Repositório Científico do Instituto Nacional de Saúde |
dc.contributor.author.fl_str_mv |
Dias, Elsa Oliveira, Micaela Jones-Dias, Daniela Vasconcelos, Vitor Ferreira, Eugénia Manageiro, Vera Caniça, Manuela |
dc.subject.por.fl_str_mv |
Cyanobacteria Antibiotics Minimum Inhibitory Concentrations Reduced Susceptibility Water Resistência aos Antimicrobianos Água e Solo |
topic |
Cyanobacteria Antibiotics Minimum Inhibitory Concentrations Reduced Susceptibility Water Resistência aos Antimicrobianos Água e Solo |
description |
Freshwater is a vehicle for the emergence and dissemination of Antibiotic resistance. Cyanobacteria are ubiquitous in freshwater, where they are exposed to antibiotics and resistant organisms, but their role on water resistome was never evaluated. Data concerning the effects of antibiotics on cyanobacteria, obtained by distinct methodologies, is often contradictory. This emphasizes the importance of developing procedures to understand the trends of antibiotic susceptibility in cyanobacteria. In this study we aimed to evaluate the susceptibility of four cyanobacterial isolates from different genera (Microcystis aeruginosa, Aphanizomenon gracile, Chrisosporum bergii, Planktothix agradhii), and among them nine isolates from the same specie (M.aeruginosa) to distinct antibiotics (amoxicillin, ceftazidime, ceftriaxone, kanamycine, gentamicine, tetracycline, trimethoprim, nalidixicacid, norfloxacin). We used a method adapted from the bacteria standard broth microdilution. Cyanobacteria were exposed to serial dilution of each antibiotic (0.0015–1.6mg/L) in Z8 medium (20±1◦C; 14/10hL/D cycle; light intensity 16±421μEm−s−). Cell growth was followed overtime (OD450nm/microscopic examination) and the minimum inhibitory concentrations (MICs) were calculated for each antibiotic/ isolate. We found that β-lactams exhibited the lower MICs, aminoglycosides, tetracycline and norfloxacine presented intermediate MICs; none of the isolates were susceptible to trimethoprim and nalidixic acid. The reduced susceptibility of all tested cyanobacteria to some antibiotics suggests that they might be naturally non-susceptible to these compounds, or that they might became non-susceptible due to antibiotic contamination pressure, or to the transfer of genes from resistant bacteria present in the environment. |
publishDate |
2015 |
dc.date.none.fl_str_mv |
2015-08-11 2015-08-11T00:00:00Z 2016-03-01T17:04:02Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10400.18/3557 |
url |
http://hdl.handle.net/10400.18/3557 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Front Microbiol. 2015 Aug 11;6:799. doi: 10.3389/fmicb.2015.00799. eCollection 2015 1664-302X 10.3389/fmicb.2015.00799 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Frontiers Media |
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Frontiers Media |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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