Expression of NR1/NR2B N-Methyl-d-Aspartate Receptors Enhances Heroin Toxicity in HEK293 Cells
Autor(a) principal: | |
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Data de Publicação: | 2012 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10316/18514 https://doi.org/10.1196/annals.1369.046 |
Resumo: | Repeated use of drugs of abuse, namely opiates, has been shown to affect glutamate-releasing neurons. Moreover, blockade of N-methyl-D-aspartate (NMDA) receptors (NMDAR) prevents cell death by apoptosis induced by morphine, a heroin metabolite. Thus, in this article we investigated the involvement of different NMDAR subunits in heroin cytotoxicity. Human embryonic kidney (HEK293)cells, which do not express native NMDAR, were transfected with NR1/NR2A or NR1/NR2B subunits. As a control, cells were transfected with NR1 alone, which does not form functional channels. Incubation with heroin for 24 h induced a dose-dependent decrease in cell viability both in NR1-transfected and nontransfected cells. The loss of membrane integrity induced by heroin was more evident in cells transfected with NR1/NR2B than in cells transfected with NR1 alone or NR1/NR2A. This decrease in cell viability was blocked by MK-801, a selective and noncompetitive antagonist of NMDAR. Nevertheless, no significant changes in intracellular adenosine 5′-triphosphate (ATP) were observed in cells treated with heroin. These data implicate NR2B-composed NMDAR as important mediators of heroin neurotoxicity. |
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Expression of NR1/NR2B N-Methyl-d-Aspartate Receptors Enhances Heroin Toxicity in HEK293 CellsRepeated use of drugs of abuse, namely opiates, has been shown to affect glutamate-releasing neurons. Moreover, blockade of N-methyl-D-aspartate (NMDA) receptors (NMDAR) prevents cell death by apoptosis induced by morphine, a heroin metabolite. Thus, in this article we investigated the involvement of different NMDAR subunits in heroin cytotoxicity. Human embryonic kidney (HEK293)cells, which do not express native NMDAR, were transfected with NR1/NR2A or NR1/NR2B subunits. As a control, cells were transfected with NR1 alone, which does not form functional channels. Incubation with heroin for 24 h induced a dose-dependent decrease in cell viability both in NR1-transfected and nontransfected cells. The loss of membrane integrity induced by heroin was more evident in cells transfected with NR1/NR2B than in cells transfected with NR1 alone or NR1/NR2A. This decrease in cell viability was blocked by MK-801, a selective and noncompetitive antagonist of NMDAR. Nevertheless, no significant changes in intracellular adenosine 5′-triphosphate (ATP) were observed in cells treated with heroin. These data implicate NR2B-composed NMDAR as important mediators of heroin neurotoxicity.2012-03-08info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://hdl.handle.net/10316/18514http://hdl.handle.net/10316/18514https://doi.org/10.1196/annals.1369.046enghttp://onlinelibrary.wiley.com/doi/10.1196/annals.1369.046/abstractDomingues, AntónioCunha-Oliveira, TeresaLaço, Mário L. N.Macedo, TiceOliveira, Catarina R.Rego, A. Cristinainfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2021-10-06T08:10:41Zoai:estudogeral.uc.pt:10316/18514Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T20:53:34.144428Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Expression of NR1/NR2B N-Methyl-d-Aspartate Receptors Enhances Heroin Toxicity in HEK293 Cells |
title |
Expression of NR1/NR2B N-Methyl-d-Aspartate Receptors Enhances Heroin Toxicity in HEK293 Cells |
spellingShingle |
Expression of NR1/NR2B N-Methyl-d-Aspartate Receptors Enhances Heroin Toxicity in HEK293 Cells Domingues, António |
title_short |
Expression of NR1/NR2B N-Methyl-d-Aspartate Receptors Enhances Heroin Toxicity in HEK293 Cells |
title_full |
Expression of NR1/NR2B N-Methyl-d-Aspartate Receptors Enhances Heroin Toxicity in HEK293 Cells |
title_fullStr |
Expression of NR1/NR2B N-Methyl-d-Aspartate Receptors Enhances Heroin Toxicity in HEK293 Cells |
title_full_unstemmed |
Expression of NR1/NR2B N-Methyl-d-Aspartate Receptors Enhances Heroin Toxicity in HEK293 Cells |
title_sort |
Expression of NR1/NR2B N-Methyl-d-Aspartate Receptors Enhances Heroin Toxicity in HEK293 Cells |
author |
Domingues, António |
author_facet |
Domingues, António Cunha-Oliveira, Teresa Laço, Mário L. N. Macedo, Tice Oliveira, Catarina R. Rego, A. Cristina |
author_role |
author |
author2 |
Cunha-Oliveira, Teresa Laço, Mário L. N. Macedo, Tice Oliveira, Catarina R. Rego, A. Cristina |
author2_role |
author author author author author |
dc.contributor.author.fl_str_mv |
Domingues, António Cunha-Oliveira, Teresa Laço, Mário L. N. Macedo, Tice Oliveira, Catarina R. Rego, A. Cristina |
description |
Repeated use of drugs of abuse, namely opiates, has been shown to affect glutamate-releasing neurons. Moreover, blockade of N-methyl-D-aspartate (NMDA) receptors (NMDAR) prevents cell death by apoptosis induced by morphine, a heroin metabolite. Thus, in this article we investigated the involvement of different NMDAR subunits in heroin cytotoxicity. Human embryonic kidney (HEK293)cells, which do not express native NMDAR, were transfected with NR1/NR2A or NR1/NR2B subunits. As a control, cells were transfected with NR1 alone, which does not form functional channels. Incubation with heroin for 24 h induced a dose-dependent decrease in cell viability both in NR1-transfected and nontransfected cells. The loss of membrane integrity induced by heroin was more evident in cells transfected with NR1/NR2B than in cells transfected with NR1 alone or NR1/NR2A. This decrease in cell viability was blocked by MK-801, a selective and noncompetitive antagonist of NMDAR. Nevertheless, no significant changes in intracellular adenosine 5′-triphosphate (ATP) were observed in cells treated with heroin. These data implicate NR2B-composed NMDAR as important mediators of heroin neurotoxicity. |
publishDate |
2012 |
dc.date.none.fl_str_mv |
2012-03-08 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10316/18514 http://hdl.handle.net/10316/18514 https://doi.org/10.1196/annals.1369.046 |
url |
http://hdl.handle.net/10316/18514 https://doi.org/10.1196/annals.1369.046 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
http://onlinelibrary.wiley.com/doi/10.1196/annals.1369.046/abstract |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
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reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
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Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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RCAAP |
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RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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1799133822308057088 |