Evaluation of DNA levels recovered from forensic bone samples through the optimization of a semi-automated extraction method

Detalhes bibliográficos
Autor(a) principal: Caldeira, M.J.
Data de Publicação: 2019
Outros Autores: Bento, A.M., Gouveia, N., Brito, P., Porto, M.J.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10773/37177
Resumo: DNA was extracted from eleven bone samples using PrepFiler Express BTA™ Forensic DNA Extraction kit with AutoMate Express™ Forensic DNA Extraction System with 20 and 50 μL of elution volume. The objective was to compare extraction efficiency with different elution volumes, evaluating DNA recovery, profile quality and observing if sample concentration alters the degradation index (DI) and the presence of inhibitors. The analyzed samples showed increased DNA levels with the elution volume of 20 μL, and the inhibition values did not change among the protocols, however some changes in the DI were observed. Undesirably, for low DNA concentrations, the 20 μL protocol demands more extractions per sample. This 20 μL method proved to be useful for difficult samples with low levels of DNA and high degradation, being able to increase DNA concentration and obtain genetic profiles with higher quality compared to the protocol in use, increasing the efficiency of analysis, important in cases of disaster victim identification (DVI).
id RCAP_ebe5262ac4ba53932a277319df7fe3fd
oai_identifier_str oai:ria.ua.pt:10773/37177
network_acronym_str RCAP
network_name_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository_id_str 7160
spelling Evaluation of DNA levels recovered from forensic bone samples through the optimization of a semi-automated extraction methodDVIElution volumeDNAExtractionDNA was extracted from eleven bone samples using PrepFiler Express BTA™ Forensic DNA Extraction kit with AutoMate Express™ Forensic DNA Extraction System with 20 and 50 μL of elution volume. The objective was to compare extraction efficiency with different elution volumes, evaluating DNA recovery, profile quality and observing if sample concentration alters the degradation index (DI) and the presence of inhibitors. The analyzed samples showed increased DNA levels with the elution volume of 20 μL, and the inhibition values did not change among the protocols, however some changes in the DI were observed. Undesirably, for low DNA concentrations, the 20 μL protocol demands more extractions per sample. This 20 μL method proved to be useful for difficult samples with low levels of DNA and high degradation, being able to increase DNA concentration and obtain genetic profiles with higher quality compared to the protocol in use, increasing the efficiency of analysis, important in cases of disaster victim identification (DVI).Elsevier2023-04-18T14:48:39Z2019-12-01T00:00:00Z2019-12info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10773/37177eng1875-176810.1016/j.fsigss.2019.10.111Caldeira, M.J.Bento, A.M.Gouveia, N.Brito, P.Porto, M.J.info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-02-22T12:11:41Zoai:ria.ua.pt:10773/37177Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T03:07:48.087859Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Evaluation of DNA levels recovered from forensic bone samples through the optimization of a semi-automated extraction method
title Evaluation of DNA levels recovered from forensic bone samples through the optimization of a semi-automated extraction method
spellingShingle Evaluation of DNA levels recovered from forensic bone samples through the optimization of a semi-automated extraction method
Caldeira, M.J.
DVI
Elution volume
DNA
Extraction
title_short Evaluation of DNA levels recovered from forensic bone samples through the optimization of a semi-automated extraction method
title_full Evaluation of DNA levels recovered from forensic bone samples through the optimization of a semi-automated extraction method
title_fullStr Evaluation of DNA levels recovered from forensic bone samples through the optimization of a semi-automated extraction method
title_full_unstemmed Evaluation of DNA levels recovered from forensic bone samples through the optimization of a semi-automated extraction method
title_sort Evaluation of DNA levels recovered from forensic bone samples through the optimization of a semi-automated extraction method
author Caldeira, M.J.
author_facet Caldeira, M.J.
Bento, A.M.
Gouveia, N.
Brito, P.
Porto, M.J.
author_role author
author2 Bento, A.M.
Gouveia, N.
Brito, P.
Porto, M.J.
author2_role author
author
author
author
dc.contributor.author.fl_str_mv Caldeira, M.J.
Bento, A.M.
Gouveia, N.
Brito, P.
Porto, M.J.
dc.subject.por.fl_str_mv DVI
Elution volume
DNA
Extraction
topic DVI
Elution volume
DNA
Extraction
description DNA was extracted from eleven bone samples using PrepFiler Express BTA™ Forensic DNA Extraction kit with AutoMate Express™ Forensic DNA Extraction System with 20 and 50 μL of elution volume. The objective was to compare extraction efficiency with different elution volumes, evaluating DNA recovery, profile quality and observing if sample concentration alters the degradation index (DI) and the presence of inhibitors. The analyzed samples showed increased DNA levels with the elution volume of 20 μL, and the inhibition values did not change among the protocols, however some changes in the DI were observed. Undesirably, for low DNA concentrations, the 20 μL protocol demands more extractions per sample. This 20 μL method proved to be useful for difficult samples with low levels of DNA and high degradation, being able to increase DNA concentration and obtain genetic profiles with higher quality compared to the protocol in use, increasing the efficiency of analysis, important in cases of disaster victim identification (DVI).
publishDate 2019
dc.date.none.fl_str_mv 2019-12-01T00:00:00Z
2019-12
2023-04-18T14:48:39Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10773/37177
url http://hdl.handle.net/10773/37177
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 1875-1768
10.1016/j.fsigss.2019.10.111
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
repository.mail.fl_str_mv
_version_ 1799137731839787008

Registros relacionados