BSA-based sample clean-up columns for ochratoxin A determination in wine: method development and validation
Autor(a) principal: | |
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Data de Publicação: | 2019 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/1822/60971 |
Resumo: | Analytical chromatographic techniques for mycotoxins control are well established, but they often depend on costly immunoaffinity sample clean-up. Serum albumins, particularly that from bovine origin (BSA), have stable binding affinity towards some mycotoxins, and can be cheaper alternative receptors for sample clean-up due to their wide availability. Thus, this work used BSA immobilized in agarose beads as a novel solid-phase extraction method for quantification of ochratoxin A (OTA) in wine. Constructed BSA-agarose columns could extract OTA efficiently from red wine after its dilution (4-fold) in 0.1 M Tris pH 8.0. The method was linear (R2=0.9999) in the OTA concentration range studied (0.05 to 3.0 g L-1), with recovery rates above 98%. It also showed low detection (0.017 g L-1) and quantification (0.051 g L-1) limits. The efficacy of the BSA-based method was further validated by direct comparison with commercial immunoaffinity columns. Portuguese wines analyzed by both methods had agreeing results. |
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BSA-based sample clean-up columns for ochratoxin A determination in wine: method development and validationOchratoxin Abovine serum albuminsolid-phase extractionwine analysismethod validationScience & TechnologyAnalytical chromatographic techniques for mycotoxins control are well established, but they often depend on costly immunoaffinity sample clean-up. Serum albumins, particularly that from bovine origin (BSA), have stable binding affinity towards some mycotoxins, and can be cheaper alternative receptors for sample clean-up due to their wide availability. Thus, this work used BSA immobilized in agarose beads as a novel solid-phase extraction method for quantification of ochratoxin A (OTA) in wine. Constructed BSA-agarose columns could extract OTA efficiently from red wine after its dilution (4-fold) in 0.1 M Tris pH 8.0. The method was linear (R2=0.9999) in the OTA concentration range studied (0.05 to 3.0 g L-1), with recovery rates above 98%. It also showed low detection (0.017 g L-1) and quantification (0.051 g L-1) limits. The efficacy of the BSA-based method was further validated by direct comparison with commercial immunoaffinity columns. Portuguese wines analyzed by both methods had agreeing results.This study was supported by the Portuguese Foundation for Scienceand Technology (FCT) under the scope of the strategic funding of UID/BIO/04469 unit and COMPETE 2020 (POCI-01-0145-FEDER-006684)and BioTecNorte operation (NORTE-01-0145-FEDER-000004) funded by the European Regional Development Fund under the scope of Norte2020 - Programa Operacional Regional do Norte. Luís Abrunhosawas supported by grant UMINHO/BPD/51/2015 from project UID/BIO/04469/2013 financed by FCT/MEC (OE). Carla Oliveira was sup-ported by the FCT grant SFRH/BPD/110640/2015.info:eu-repo/semantics/publishedVersionElsevierUniversidade do MinhoLeal, TâniaAbrunhosa, LuísDomingues, LucíliaVenâncio, ArmandoOliveira, Carla2019-122019-12-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/1822/60971engLeal, Tânia; Abrunhosa, Luís; Domingues, Lucília; Venâncio, Armando; Oliveira, Carla, BSA-based sample clean-up columns for ochratoxin A determination in wine: method development and validation. Food Chemistry, 300(125204), 20190308-814610.1016/j.foodchem.2019.12520431336275http://www.elsevier.com/locate/issn/03088146info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-21T12:15:51Zoai:repositorium.sdum.uminho.pt:1822/60971Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T19:08:22.518955Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
BSA-based sample clean-up columns for ochratoxin A determination in wine: method development and validation |
title |
BSA-based sample clean-up columns for ochratoxin A determination in wine: method development and validation |
spellingShingle |
BSA-based sample clean-up columns for ochratoxin A determination in wine: method development and validation Leal, Tânia Ochratoxin A bovine serum albumin solid-phase extraction wine analysis method validation Science & Technology |
title_short |
BSA-based sample clean-up columns for ochratoxin A determination in wine: method development and validation |
title_full |
BSA-based sample clean-up columns for ochratoxin A determination in wine: method development and validation |
title_fullStr |
BSA-based sample clean-up columns for ochratoxin A determination in wine: method development and validation |
title_full_unstemmed |
BSA-based sample clean-up columns for ochratoxin A determination in wine: method development and validation |
title_sort |
BSA-based sample clean-up columns for ochratoxin A determination in wine: method development and validation |
author |
Leal, Tânia |
author_facet |
Leal, Tânia Abrunhosa, Luís Domingues, Lucília Venâncio, Armando Oliveira, Carla |
author_role |
author |
author2 |
Abrunhosa, Luís Domingues, Lucília Venâncio, Armando Oliveira, Carla |
author2_role |
author author author author |
dc.contributor.none.fl_str_mv |
Universidade do Minho |
dc.contributor.author.fl_str_mv |
Leal, Tânia Abrunhosa, Luís Domingues, Lucília Venâncio, Armando Oliveira, Carla |
dc.subject.por.fl_str_mv |
Ochratoxin A bovine serum albumin solid-phase extraction wine analysis method validation Science & Technology |
topic |
Ochratoxin A bovine serum albumin solid-phase extraction wine analysis method validation Science & Technology |
description |
Analytical chromatographic techniques for mycotoxins control are well established, but they often depend on costly immunoaffinity sample clean-up. Serum albumins, particularly that from bovine origin (BSA), have stable binding affinity towards some mycotoxins, and can be cheaper alternative receptors for sample clean-up due to their wide availability. Thus, this work used BSA immobilized in agarose beads as a novel solid-phase extraction method for quantification of ochratoxin A (OTA) in wine. Constructed BSA-agarose columns could extract OTA efficiently from red wine after its dilution (4-fold) in 0.1 M Tris pH 8.0. The method was linear (R2=0.9999) in the OTA concentration range studied (0.05 to 3.0 g L-1), with recovery rates above 98%. It also showed low detection (0.017 g L-1) and quantification (0.051 g L-1) limits. The efficacy of the BSA-based method was further validated by direct comparison with commercial immunoaffinity columns. Portuguese wines analyzed by both methods had agreeing results. |
publishDate |
2019 |
dc.date.none.fl_str_mv |
2019-12 2019-12-01T00:00:00Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/1822/60971 |
url |
http://hdl.handle.net/1822/60971 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Leal, Tânia; Abrunhosa, Luís; Domingues, Lucília; Venâncio, Armando; Oliveira, Carla, BSA-based sample clean-up columns for ochratoxin A determination in wine: method development and validation. Food Chemistry, 300(125204), 2019 0308-8146 10.1016/j.foodchem.2019.125204 31336275 http://www.elsevier.com/locate/issn/03088146 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Elsevier |
publisher.none.fl_str_mv |
Elsevier |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
instname_str |
Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
instacron_str |
RCAAP |
institution |
RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
collection |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository.name.fl_str_mv |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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1799132505589153792 |