Dynamics of Microbial Communities in Phototrophic Polyhydroxyalkanoate Accumulating Cultures

Detalhes bibliográficos
Autor(a) principal: Almeida, Juliana R.
Data de Publicação: 2022
Outros Autores: Fradinho, Joana C., Carvalho, Gilda, Oehmen, Adrian, Reis, Maria A. M.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10362/133196
Resumo: DFA/BD/8201/2020 UIDP/04378/2020 UIDB/04378/2020 LA/P/0140/2020
id RCAP_ecf3b7538590ddc3df0aaa3e8c11e1a2
oai_identifier_str oai:run.unl.pt:10362/133196
network_acronym_str RCAP
network_name_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository_id_str 7160
spelling Dynamics of Microbial Communities in Phototrophic Polyhydroxyalkanoate Accumulating CulturesDNA sequencingDomestic wastewaterFeast and famine (FF)Fluorescence in situ hybridization (FISH)Microbial communitiesPermanent feast (PF)Phototrophic mixed cultures (PMC)Phototrophic purple bacteria (PPB)Polyhydroxyalkanoates (PHA)MicrobiologyMicrobiology (medical)VirologyDFA/BD/8201/2020 UIDP/04378/2020 UIDB/04378/2020 LA/P/0140/2020Phototrophic mixed cultures (PMC) are versatile systems which can be applied for waste streams, valorisation and production of added-value compounds, such as polyhydroxyalkanoates (PHA). This work evaluates the influence of different operational conditions on the bacterial communities reported in PMC systems with PHA production capabilities. Eleven PMCs, fed either with acetate or fermented wastewater, and selected under either feast and famine (FF) or permanent feast (PF) regimes, were evaluated. Overall, results identified Chromatiaceae members as the main phototrophic PHA producers, along with Rhodopseudomonas, Rhodobacter and Rhizobium. The findings show that Chromatiaceae were favoured under operating conditions with high carbon concentrations, and particularly under the PF regime. In FF systems fed with fermented wastewater, the results indicate that increasing the organic loading rate enriches for Rhodopseudomonas, Rhizobium and Hyphomicrobiaceae, which together with Rhodobacter and Chromatiaceae, were likely responsible for PHA storage. In addition, high-sugar feedstock impairs PHA production under PF conditions (fermentative bacteria dominance), which does not occur under FF. This characterization of the communities responsible for PHA accumulation helps to define improved operational strategies for PHA production with PMC.UCIBIO - Applied Molecular Biosciences UnitDQ - Departamento de QuímicaRUNAlmeida, Juliana R.Fradinho, Joana C.Carvalho, GildaOehmen, AdrianReis, Maria A. M.2022-02-18T23:19:06Z2022-02-032022-02-03T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10362/133196eng2076-2607PURE: 36690330https://doi.org/10.3390/microorganisms10020351info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-03-11T05:11:54Zoai:run.unl.pt:10362/133196Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T03:47:44.659761Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Dynamics of Microbial Communities in Phototrophic Polyhydroxyalkanoate Accumulating Cultures
title Dynamics of Microbial Communities in Phototrophic Polyhydroxyalkanoate Accumulating Cultures
spellingShingle Dynamics of Microbial Communities in Phototrophic Polyhydroxyalkanoate Accumulating Cultures
Almeida, Juliana R.
DNA sequencing
Domestic wastewater
Feast and famine (FF)
Fluorescence in situ hybridization (FISH)
Microbial communities
Permanent feast (PF)
Phototrophic mixed cultures (PMC)
Phototrophic purple bacteria (PPB)
Polyhydroxyalkanoates (PHA)
Microbiology
Microbiology (medical)
Virology
title_short Dynamics of Microbial Communities in Phototrophic Polyhydroxyalkanoate Accumulating Cultures
title_full Dynamics of Microbial Communities in Phototrophic Polyhydroxyalkanoate Accumulating Cultures
title_fullStr Dynamics of Microbial Communities in Phototrophic Polyhydroxyalkanoate Accumulating Cultures
title_full_unstemmed Dynamics of Microbial Communities in Phototrophic Polyhydroxyalkanoate Accumulating Cultures
title_sort Dynamics of Microbial Communities in Phototrophic Polyhydroxyalkanoate Accumulating Cultures
author Almeida, Juliana R.
author_facet Almeida, Juliana R.
Fradinho, Joana C.
Carvalho, Gilda
Oehmen, Adrian
Reis, Maria A. M.
author_role author
author2 Fradinho, Joana C.
Carvalho, Gilda
Oehmen, Adrian
Reis, Maria A. M.
author2_role author
author
author
author
dc.contributor.none.fl_str_mv UCIBIO - Applied Molecular Biosciences Unit
DQ - Departamento de Química
RUN
dc.contributor.author.fl_str_mv Almeida, Juliana R.
Fradinho, Joana C.
Carvalho, Gilda
Oehmen, Adrian
Reis, Maria A. M.
dc.subject.por.fl_str_mv DNA sequencing
Domestic wastewater
Feast and famine (FF)
Fluorescence in situ hybridization (FISH)
Microbial communities
Permanent feast (PF)
Phototrophic mixed cultures (PMC)
Phototrophic purple bacteria (PPB)
Polyhydroxyalkanoates (PHA)
Microbiology
Microbiology (medical)
Virology
topic DNA sequencing
Domestic wastewater
Feast and famine (FF)
Fluorescence in situ hybridization (FISH)
Microbial communities
Permanent feast (PF)
Phototrophic mixed cultures (PMC)
Phototrophic purple bacteria (PPB)
Polyhydroxyalkanoates (PHA)
Microbiology
Microbiology (medical)
Virology
description DFA/BD/8201/2020 UIDP/04378/2020 UIDB/04378/2020 LA/P/0140/2020
publishDate 2022
dc.date.none.fl_str_mv 2022-02-18T23:19:06Z
2022-02-03
2022-02-03T00:00:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10362/133196
url http://hdl.handle.net/10362/133196
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 2076-2607
PURE: 36690330
https://doi.org/10.3390/microorganisms10020351
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
repository.mail.fl_str_mv
_version_ 1799138080127451136

Registros relacionados