Improving polyhydroxyalkanoates production in phototrophic mixed cultures by optimizing accumulator reactor operating conditions

Detalhes bibliográficos
Autor(a) principal: Fradinho, J. C.
Data de Publicação: 2019
Outros Autores: Oehmen, A., Reis, M. A. M.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10362/98795
Resumo: The authors would also like to acknowledge the Fundacao para a Ciencia e a Tecnologia (Portugal) for funding through SFRH/BPD/101642/2014. co-financed by ERDF under PT2020 Partnership Agreement (POCI-01-0145-FEDER-007728).
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spelling Improving polyhydroxyalkanoates production in phototrophic mixed cultures by optimizing accumulator reactor operating conditionsFermented cheese whey (FCW)Phototrophic mixed cultures (PMCs)Polyhydroxyalkanoates (PHA)Purple phototrophic bacteria (PPB)Volatile fatty acids (VFAs)Structural BiologyBiochemistryMolecular BiologyThe authors would also like to acknowledge the Fundacao para a Ciencia e a Tecnologia (Portugal) for funding through SFRH/BPD/101642/2014. co-financed by ERDF under PT2020 Partnership Agreement (POCI-01-0145-FEDER-007728).Polyhydroxyalkanoates (PHAs) production with phototrophic mixed cultures (PMCs) has been recently proposed. These cultures can be selected under the permanent presence of carbon and the PHA production can be enhanced in subsequent accumulation steps. To optimize the PHA production in accumulator reactors, this work evaluated the impact of 1) initial acetate concentration, 2) light intensity, 3) removal of residual nitrogen on the culture performance. Results indicate that low acetate concentration (<30 CmM) and specific light intensities around 20 W/gX are optimal operating conditions that lead to high polyhydroxybutyrate (PHB) storage yields (0.83 ± 0.07 Cmol-PHB/Cmol-Acet) and specific PHB production rates of 2.21 ± 0.07 Cmol-PHB/Cmol X d. This rate is three times higher than previously registered in non-optimized accumulation tests and enabled a PHA content increase from 15 to 30% in <4 h. Also, it was shown for the first time, the capability of a PMC to use a real waste, fermented cheese whey, to produce PHA with a hydroxyvalerate (HV) content of 12%. These results confirm that fermented wastes can be used as substrates for PHA production with PMCs and that the energy levels in sunlight that lead to specific light intensities from 10 to 20 W/gX are sufficient to drive phototrophic PHA production processes.UCIBIO - Applied Molecular Biosciences UnitDQ - Departamento de QuímicaRUNFradinho, J. C.Oehmen, A.Reis, M. A. M.2020-06-03T10:25:41Z2019-04-012019-04-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article8application/pdfhttp://hdl.handle.net/10362/98795eng0141-8130PURE: 11474735https://doi.org/10.1016/j.ijbiomac.2018.12.270info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-03-11T04:45:59Zoai:run.unl.pt:10362/98795Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T03:39:03.273362Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Improving polyhydroxyalkanoates production in phototrophic mixed cultures by optimizing accumulator reactor operating conditions
title Improving polyhydroxyalkanoates production in phototrophic mixed cultures by optimizing accumulator reactor operating conditions
spellingShingle Improving polyhydroxyalkanoates production in phototrophic mixed cultures by optimizing accumulator reactor operating conditions
Fradinho, J. C.
Fermented cheese whey (FCW)
Phototrophic mixed cultures (PMCs)
Polyhydroxyalkanoates (PHA)
Purple phototrophic bacteria (PPB)
Volatile fatty acids (VFAs)
Structural Biology
Biochemistry
Molecular Biology
title_short Improving polyhydroxyalkanoates production in phototrophic mixed cultures by optimizing accumulator reactor operating conditions
title_full Improving polyhydroxyalkanoates production in phototrophic mixed cultures by optimizing accumulator reactor operating conditions
title_fullStr Improving polyhydroxyalkanoates production in phototrophic mixed cultures by optimizing accumulator reactor operating conditions
title_full_unstemmed Improving polyhydroxyalkanoates production in phototrophic mixed cultures by optimizing accumulator reactor operating conditions
title_sort Improving polyhydroxyalkanoates production in phototrophic mixed cultures by optimizing accumulator reactor operating conditions
author Fradinho, J. C.
author_facet Fradinho, J. C.
Oehmen, A.
Reis, M. A. M.
author_role author
author2 Oehmen, A.
Reis, M. A. M.
author2_role author
author
dc.contributor.none.fl_str_mv UCIBIO - Applied Molecular Biosciences Unit
DQ - Departamento de Química
RUN
dc.contributor.author.fl_str_mv Fradinho, J. C.
Oehmen, A.
Reis, M. A. M.
dc.subject.por.fl_str_mv Fermented cheese whey (FCW)
Phototrophic mixed cultures (PMCs)
Polyhydroxyalkanoates (PHA)
Purple phototrophic bacteria (PPB)
Volatile fatty acids (VFAs)
Structural Biology
Biochemistry
Molecular Biology
topic Fermented cheese whey (FCW)
Phototrophic mixed cultures (PMCs)
Polyhydroxyalkanoates (PHA)
Purple phototrophic bacteria (PPB)
Volatile fatty acids (VFAs)
Structural Biology
Biochemistry
Molecular Biology
description The authors would also like to acknowledge the Fundacao para a Ciencia e a Tecnologia (Portugal) for funding through SFRH/BPD/101642/2014. co-financed by ERDF under PT2020 Partnership Agreement (POCI-01-0145-FEDER-007728).
publishDate 2019
dc.date.none.fl_str_mv 2019-04-01
2019-04-01T00:00:00Z
2020-06-03T10:25:41Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10362/98795
url http://hdl.handle.net/10362/98795
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 0141-8130
PURE: 11474735
https://doi.org/10.1016/j.ijbiomac.2018.12.270
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 8
application/pdf
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
repository.mail.fl_str_mv
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