Core microbial functional activities in ocean environments revealed by global metagenomic profiling analyses
Autor(a) principal: | |
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Data de Publicação: | 2014 |
Outros Autores: | , , , , , , , , , , , , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/1822/31508 |
Resumo: | Metagenomics-based functional profiling analysis is an effective means of gaining deeper insight into the composition of marine microbial populations and developing a better understanding of the interplay between the functional genome content of microbial communities and abiotic factors. Here we present a comprehensive analysis of 24 datasets covering surface and depth-related environments at 11 sites around the world's oceans. The complete datasets comprises approximately 12 million sequences, totaling 5,358 Mb. Based on profiling patterns of Clusters of Orthologous Groups (COGs) of proteins, a core set of reference photic and aphotic depth-related COGs, and a collection of COGs that are associated with extreme oxygen limitation were defined. Their inferred functions were utilized as indicators to characterize the distribution of light- and oxygen-related biological activities in marine environments. The results reveal that, while light level in the water column is a major determinant of phenotypic adaptation in marine microorganisms, oxygen concentration in the aphotic zone has a significant impact only in extremely hypoxic waters. Phylogenetic profiling of the reference photic/aphotic gene sets revealed a greater variety of source organisms in the aphotic zone, although the majority of individual photic and aphotic depth-related COGs are assigned to the same taxa across the different sites. This increase in phylogenetic and functional diversity of the core aphotic related COGs most probably reflects selection for the utilization of a broad range of alternate energy sources in the absence of light. |
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Core microbial functional activities in ocean environments revealed by global metagenomic profiling analysesScience & TechnologyMetagenomics-based functional profiling analysis is an effective means of gaining deeper insight into the composition of marine microbial populations and developing a better understanding of the interplay between the functional genome content of microbial communities and abiotic factors. Here we present a comprehensive analysis of 24 datasets covering surface and depth-related environments at 11 sites around the world's oceans. The complete datasets comprises approximately 12 million sequences, totaling 5,358 Mb. Based on profiling patterns of Clusters of Orthologous Groups (COGs) of proteins, a core set of reference photic and aphotic depth-related COGs, and a collection of COGs that are associated with extreme oxygen limitation were defined. Their inferred functions were utilized as indicators to characterize the distribution of light- and oxygen-related biological activities in marine environments. The results reveal that, while light level in the water column is a major determinant of phenotypic adaptation in marine microorganisms, oxygen concentration in the aphotic zone has a significant impact only in extremely hypoxic waters. Phylogenetic profiling of the reference photic/aphotic gene sets revealed a greater variety of source organisms in the aphotic zone, although the majority of individual photic and aphotic depth-related COGs are assigned to the same taxa across the different sites. This increase in phylogenetic and functional diversity of the core aphotic related COGs most probably reflects selection for the utilization of a broad range of alternate energy sources in the absence of light.This work was supported by King Abdullah University for Science and Technology Global Collaborative Partners (GCR) program. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.Public Library of ScienceUniversidade do MinhoFerreira, Ari J. S.Siam, RaniaSetubal, João C.Moustafa, AhmedSayed, AhmedChambergo, Felipe S.Dawe, Adam S.Ghazy, Mohamed A.Sharaf, HazemOuf, AmgedAlam, IntikhabAbdel-Haleem, Alyaa M.Lehvaslaiho, HeikkiRamadan, EmanAntunes, André Guimarães LemosStingl, UlrichArcher, John A. C.Jankovic, Boris R.Sogin, MitchellBajic, Vladimir B.El-Dorry, Hamza2014-062014-06-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/1822/31508eng1932-62031932-620310.1371/journal.pone.009733824921648info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-21T12:28:42Zoai:repositorium.sdum.uminho.pt:1822/31508Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T19:23:33.850765Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Core microbial functional activities in ocean environments revealed by global metagenomic profiling analyses |
title |
Core microbial functional activities in ocean environments revealed by global metagenomic profiling analyses |
spellingShingle |
Core microbial functional activities in ocean environments revealed by global metagenomic profiling analyses Ferreira, Ari J. S. Science & Technology |
title_short |
Core microbial functional activities in ocean environments revealed by global metagenomic profiling analyses |
title_full |
Core microbial functional activities in ocean environments revealed by global metagenomic profiling analyses |
title_fullStr |
Core microbial functional activities in ocean environments revealed by global metagenomic profiling analyses |
title_full_unstemmed |
Core microbial functional activities in ocean environments revealed by global metagenomic profiling analyses |
title_sort |
Core microbial functional activities in ocean environments revealed by global metagenomic profiling analyses |
author |
Ferreira, Ari J. S. |
author_facet |
Ferreira, Ari J. S. Siam, Rania Setubal, João C. Moustafa, Ahmed Sayed, Ahmed Chambergo, Felipe S. Dawe, Adam S. Ghazy, Mohamed A. Sharaf, Hazem Ouf, Amged Alam, Intikhab Abdel-Haleem, Alyaa M. Lehvaslaiho, Heikki Ramadan, Eman Antunes, André Guimarães Lemos Stingl, Ulrich Archer, John A. C. Jankovic, Boris R. Sogin, Mitchell Bajic, Vladimir B. El-Dorry, Hamza |
author_role |
author |
author2 |
Siam, Rania Setubal, João C. Moustafa, Ahmed Sayed, Ahmed Chambergo, Felipe S. Dawe, Adam S. Ghazy, Mohamed A. Sharaf, Hazem Ouf, Amged Alam, Intikhab Abdel-Haleem, Alyaa M. Lehvaslaiho, Heikki Ramadan, Eman Antunes, André Guimarães Lemos Stingl, Ulrich Archer, John A. C. Jankovic, Boris R. Sogin, Mitchell Bajic, Vladimir B. El-Dorry, Hamza |
author2_role |
author author author author author author author author author author author author author author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade do Minho |
dc.contributor.author.fl_str_mv |
Ferreira, Ari J. S. Siam, Rania Setubal, João C. Moustafa, Ahmed Sayed, Ahmed Chambergo, Felipe S. Dawe, Adam S. Ghazy, Mohamed A. Sharaf, Hazem Ouf, Amged Alam, Intikhab Abdel-Haleem, Alyaa M. Lehvaslaiho, Heikki Ramadan, Eman Antunes, André Guimarães Lemos Stingl, Ulrich Archer, John A. C. Jankovic, Boris R. Sogin, Mitchell Bajic, Vladimir B. El-Dorry, Hamza |
dc.subject.por.fl_str_mv |
Science & Technology |
topic |
Science & Technology |
description |
Metagenomics-based functional profiling analysis is an effective means of gaining deeper insight into the composition of marine microbial populations and developing a better understanding of the interplay between the functional genome content of microbial communities and abiotic factors. Here we present a comprehensive analysis of 24 datasets covering surface and depth-related environments at 11 sites around the world's oceans. The complete datasets comprises approximately 12 million sequences, totaling 5,358 Mb. Based on profiling patterns of Clusters of Orthologous Groups (COGs) of proteins, a core set of reference photic and aphotic depth-related COGs, and a collection of COGs that are associated with extreme oxygen limitation were defined. Their inferred functions were utilized as indicators to characterize the distribution of light- and oxygen-related biological activities in marine environments. The results reveal that, while light level in the water column is a major determinant of phenotypic adaptation in marine microorganisms, oxygen concentration in the aphotic zone has a significant impact only in extremely hypoxic waters. Phylogenetic profiling of the reference photic/aphotic gene sets revealed a greater variety of source organisms in the aphotic zone, although the majority of individual photic and aphotic depth-related COGs are assigned to the same taxa across the different sites. This increase in phylogenetic and functional diversity of the core aphotic related COGs most probably reflects selection for the utilization of a broad range of alternate energy sources in the absence of light. |
publishDate |
2014 |
dc.date.none.fl_str_mv |
2014-06 2014-06-01T00:00:00Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/1822/31508 |
url |
http://hdl.handle.net/1822/31508 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
1932-6203 1932-6203 10.1371/journal.pone.0097338 24921648 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Public Library of Science |
publisher.none.fl_str_mv |
Public Library of Science |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
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Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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RCAAP |
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RCAAP |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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