Aroma production by Yarrowia lipolytica in airlift and stirred tank bioreactors: differences in yeast metabolism and morphology

Detalhes bibliográficos
Autor(a) principal: Braga, Adelaide
Data de Publicação: 2015
Outros Autores: Mesquita, D. P., Amaral, A. L., Ferreira, Eugénio C., Belo, Isabel
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: https://hdl.handle.net/1822/31632
Resumo: The production of γ-decalactone from castor oil in batch cultures of Yarrowia lipolytica W29 was compared in stirred tank (STR) and airlift bioreactors. Oxygen mass transfer from air to biphasic medium was characterized in both bioreactors trough correlations for kLa with power input and superficial gas velocity. Different conditions of oxygen transfer rate (OTR) were selected to perform biotransformations and for both bioreactors improvement of γ-decalactone productivity was obtained with OTR increase, with the highest value of 75 ± 10 mg L−1 h−1 being obtained in the STR for a kLa of 113 ± 5 h−1. However, a 2-fold increase in γ-decalactone concentration (around 3 g L−1) was achieved in the airlift compared to STR. Morphological characterization of the yeast cells by quantitative image analysis techniques showed that pneumatic agitation causes less impact in the cells morphology than mechanical agitation. A predominance of somewhat loose cells and quite irregular structures was observed in the STR.
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spelling Aroma production by Yarrowia lipolytica in airlift and stirred tank bioreactors: differences in yeast metabolism and morphologyAirlift bioreactorY. lipolyticagamma-decalactoneOxygen mass transfer rateCellular morphologyImage analysis?-Decalactoneγ-DecalactoneScience & TechnologyThe production of γ-decalactone from castor oil in batch cultures of Yarrowia lipolytica W29 was compared in stirred tank (STR) and airlift bioreactors. Oxygen mass transfer from air to biphasic medium was characterized in both bioreactors trough correlations for kLa with power input and superficial gas velocity. Different conditions of oxygen transfer rate (OTR) were selected to perform biotransformations and for both bioreactors improvement of γ-decalactone productivity was obtained with OTR increase, with the highest value of 75 ± 10 mg L−1 h−1 being obtained in the STR for a kLa of 113 ± 5 h−1. However, a 2-fold increase in γ-decalactone concentration (around 3 g L−1) was achieved in the airlift compared to STR. Morphological characterization of the yeast cells by quantitative image analysis techniques showed that pneumatic agitation causes less impact in the cells morphology than mechanical agitation. A predominance of somewhat loose cells and quite irregular structures was observed in the STR.The authors thank the Project 'Biolnd - Biotechnology and Bioengineering for improved Industrial and Agro-Food processes, REF. NORTE-07-0124-FEDER-000028' co-funded by the Programa Operacional Regional do Norte (ON.2 - O Novo Norte), QREN, FEDER, "BioEnv - Biotechnology and Bioengineering for a sustainable world", REF. NORTE-07-0124-FEDER-000048, co-funded by the Programa Operacional Regional do Norte (ON.2 - O Novo Norte), QREN, FEDER and Fundacao para a Ciencia e a Tecnologia (FCT) (SFRH/BD/63701/2009 PhD grant to Adelaide Braga and SFRH/BPD/82558/2011 post-doctoral grant to Daniela Mesquita) for the financial support provided and FCT Strategic Project PEst-OE/EQB/LA0023/2013.Elsevier B.V.Universidade do MinhoBraga, AdelaideMesquita, D. P.Amaral, A. L.Ferreira, Eugénio C.Belo, Isabel20152015-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttps://hdl.handle.net/1822/31632engBraga, Adelaide; Mesquita, D. P.; Amaral, A. L.; Ferreira, E. C.; Belo, Isabel, Aroma production by Yarrowia lipolytica in airlift and stirred tank bioreactors: differences in yeast metabolism and morphology. Biochemical Engineering Journal, 93, 55-62, 20151369-703X1369-703X10.1016/j.bej.2014.09.006info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-21T12:42:33Zoai:repositorium.sdum.uminho.pt:1822/31632Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T19:39:48.966795Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Aroma production by Yarrowia lipolytica in airlift and stirred tank bioreactors: differences in yeast metabolism and morphology
title Aroma production by Yarrowia lipolytica in airlift and stirred tank bioreactors: differences in yeast metabolism and morphology
spellingShingle Aroma production by Yarrowia lipolytica in airlift and stirred tank bioreactors: differences in yeast metabolism and morphology
Braga, Adelaide
Airlift bioreactor
Y. lipolytica
gamma-decalactone
Oxygen mass transfer rate
Cellular morphology
Image analysis
?-Decalactone
γ-Decalactone
Science & Technology
title_short Aroma production by Yarrowia lipolytica in airlift and stirred tank bioreactors: differences in yeast metabolism and morphology
title_full Aroma production by Yarrowia lipolytica in airlift and stirred tank bioreactors: differences in yeast metabolism and morphology
title_fullStr Aroma production by Yarrowia lipolytica in airlift and stirred tank bioreactors: differences in yeast metabolism and morphology
title_full_unstemmed Aroma production by Yarrowia lipolytica in airlift and stirred tank bioreactors: differences in yeast metabolism and morphology
title_sort Aroma production by Yarrowia lipolytica in airlift and stirred tank bioreactors: differences in yeast metabolism and morphology
author Braga, Adelaide
author_facet Braga, Adelaide
Mesquita, D. P.
Amaral, A. L.
Ferreira, Eugénio C.
Belo, Isabel
author_role author
author2 Mesquita, D. P.
Amaral, A. L.
Ferreira, Eugénio C.
Belo, Isabel
author2_role author
author
author
author
dc.contributor.none.fl_str_mv Universidade do Minho
dc.contributor.author.fl_str_mv Braga, Adelaide
Mesquita, D. P.
Amaral, A. L.
Ferreira, Eugénio C.
Belo, Isabel
dc.subject.por.fl_str_mv Airlift bioreactor
Y. lipolytica
gamma-decalactone
Oxygen mass transfer rate
Cellular morphology
Image analysis
?-Decalactone
γ-Decalactone
Science & Technology
topic Airlift bioreactor
Y. lipolytica
gamma-decalactone
Oxygen mass transfer rate
Cellular morphology
Image analysis
?-Decalactone
γ-Decalactone
Science & Technology
description The production of γ-decalactone from castor oil in batch cultures of Yarrowia lipolytica W29 was compared in stirred tank (STR) and airlift bioreactors. Oxygen mass transfer from air to biphasic medium was characterized in both bioreactors trough correlations for kLa with power input and superficial gas velocity. Different conditions of oxygen transfer rate (OTR) were selected to perform biotransformations and for both bioreactors improvement of γ-decalactone productivity was obtained with OTR increase, with the highest value of 75 ± 10 mg L−1 h−1 being obtained in the STR for a kLa of 113 ± 5 h−1. However, a 2-fold increase in γ-decalactone concentration (around 3 g L−1) was achieved in the airlift compared to STR. Morphological characterization of the yeast cells by quantitative image analysis techniques showed that pneumatic agitation causes less impact in the cells morphology than mechanical agitation. A predominance of somewhat loose cells and quite irregular structures was observed in the STR.
publishDate 2015
dc.date.none.fl_str_mv 2015
2015-01-01T00:00:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://hdl.handle.net/1822/31632
url https://hdl.handle.net/1822/31632
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Braga, Adelaide; Mesquita, D. P.; Amaral, A. L.; Ferreira, E. C.; Belo, Isabel, Aroma production by Yarrowia lipolytica in airlift and stirred tank bioreactors: differences in yeast metabolism and morphology. Biochemical Engineering Journal, 93, 55-62, 2015
1369-703X
1369-703X
10.1016/j.bej.2014.09.006
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Elsevier B.V.
publisher.none.fl_str_mv Elsevier B.V.
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
repository.mail.fl_str_mv
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