Assessment of exposure to the Penicillium glabrum complex in cork industry using complementing methods
Autor(a) principal: | |
---|---|
Data de Publicação: | 2015 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10400.18/3508 |
Resumo: | Cork oak is the second most dominant forest species in Portugal and makes this country the world leader in cork export. Occupational exposure to Chrysonilia sitophila and the Penicillium glabrum complex in cork industry is common, and the latter fungus is associated with suberosis. However, as conventional methods seem to underestimate its presence in occupational environments, the aim of our study was to see whether information obtained by polymerase chain reaction (PCR), a molecular-based method, can complement conventional findings and give a better insight into occupational exposure of cork industry workers. We assessed fungal contamination with the P. glabrum complex in three cork manufacturing plants in the outskirts of Lisbon using both conventional and molecular methods. Conventional culturing failed to detect the fungus at six sampling sites in which PCR did detect it. This confirms our assumption that the use of complementing methods can provide information for a more accurate assessment of occupational exposure to the P. glabrum complex in cork industry. |
id |
RCAP_ee77123cee6af0a534564bd74a8a5a30 |
---|---|
oai_identifier_str |
oai:repositorio.insa.pt:10400.18/3508 |
network_acronym_str |
RCAP |
network_name_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository_id_str |
7160 |
spelling |
Assessment of exposure to the Penicillium glabrum complex in cork industry using complementing methodsOcjena izloženosti kompleksu Penicillium glabrum u proizvodnji pluta s pomoću komplementarnih metodaCork OakOccupational ExposurePenicillium glabrumUngal ExposureMolecular MethodsPCRPortugalSuberosisInfecções Sistémicas e ZoonosesCork oak is the second most dominant forest species in Portugal and makes this country the world leader in cork export. Occupational exposure to Chrysonilia sitophila and the Penicillium glabrum complex in cork industry is common, and the latter fungus is associated with suberosis. However, as conventional methods seem to underestimate its presence in occupational environments, the aim of our study was to see whether information obtained by polymerase chain reaction (PCR), a molecular-based method, can complement conventional findings and give a better insight into occupational exposure of cork industry workers. We assessed fungal contamination with the P. glabrum complex in three cork manufacturing plants in the outskirts of Lisbon using both conventional and molecular methods. Conventional culturing failed to detect the fungus at six sampling sites in which PCR did detect it. This confirms our assumption that the use of complementing methods can provide information for a more accurate assessment of occupational exposure to the P. glabrum complex in cork industry.Institute for Medical Research and Occupational HealthRepositório Científico do Instituto Nacional de SaúdeViegas, C.Sabino, RaquelBotelho, D.Santos, M.Gomes, A.Q.2016-02-26T16:40:41Z2015-09-012015-09-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10400.18/3508engArh Hig Rada Toksikol. 2015 Sep 1;66(3):203-7. doi: 10.1515/aiht-2015-66-26140004-125410.1515/aiht-2015-66-2614info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-20T15:39:46Zoai:repositorio.insa.pt:10400.18/3508Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T18:38:16.762300Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Assessment of exposure to the Penicillium glabrum complex in cork industry using complementing methods Ocjena izloženosti kompleksu Penicillium glabrum u proizvodnji pluta s pomoću komplementarnih metoda |
title |
Assessment of exposure to the Penicillium glabrum complex in cork industry using complementing methods |
spellingShingle |
Assessment of exposure to the Penicillium glabrum complex in cork industry using complementing methods Viegas, C. Cork Oak Occupational Exposure Penicillium glabrum Ungal Exposure Molecular Methods PCR Portugal Suberosis Infecções Sistémicas e Zoonoses |
title_short |
Assessment of exposure to the Penicillium glabrum complex in cork industry using complementing methods |
title_full |
Assessment of exposure to the Penicillium glabrum complex in cork industry using complementing methods |
title_fullStr |
Assessment of exposure to the Penicillium glabrum complex in cork industry using complementing methods |
title_full_unstemmed |
Assessment of exposure to the Penicillium glabrum complex in cork industry using complementing methods |
title_sort |
Assessment of exposure to the Penicillium glabrum complex in cork industry using complementing methods |
author |
Viegas, C. |
author_facet |
Viegas, C. Sabino, Raquel Botelho, D. Santos, M. Gomes, A.Q. |
author_role |
author |
author2 |
Sabino, Raquel Botelho, D. Santos, M. Gomes, A.Q. |
author2_role |
author author author author |
dc.contributor.none.fl_str_mv |
Repositório Científico do Instituto Nacional de Saúde |
dc.contributor.author.fl_str_mv |
Viegas, C. Sabino, Raquel Botelho, D. Santos, M. Gomes, A.Q. |
dc.subject.por.fl_str_mv |
Cork Oak Occupational Exposure Penicillium glabrum Ungal Exposure Molecular Methods PCR Portugal Suberosis Infecções Sistémicas e Zoonoses |
topic |
Cork Oak Occupational Exposure Penicillium glabrum Ungal Exposure Molecular Methods PCR Portugal Suberosis Infecções Sistémicas e Zoonoses |
description |
Cork oak is the second most dominant forest species in Portugal and makes this country the world leader in cork export. Occupational exposure to Chrysonilia sitophila and the Penicillium glabrum complex in cork industry is common, and the latter fungus is associated with suberosis. However, as conventional methods seem to underestimate its presence in occupational environments, the aim of our study was to see whether information obtained by polymerase chain reaction (PCR), a molecular-based method, can complement conventional findings and give a better insight into occupational exposure of cork industry workers. We assessed fungal contamination with the P. glabrum complex in three cork manufacturing plants in the outskirts of Lisbon using both conventional and molecular methods. Conventional culturing failed to detect the fungus at six sampling sites in which PCR did detect it. This confirms our assumption that the use of complementing methods can provide information for a more accurate assessment of occupational exposure to the P. glabrum complex in cork industry. |
publishDate |
2015 |
dc.date.none.fl_str_mv |
2015-09-01 2015-09-01T00:00:00Z 2016-02-26T16:40:41Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10400.18/3508 |
url |
http://hdl.handle.net/10400.18/3508 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Arh Hig Rada Toksikol. 2015 Sep 1;66(3):203-7. doi: 10.1515/aiht-2015-66-2614 0004-1254 10.1515/aiht-2015-66-2614 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Institute for Medical Research and Occupational Health |
publisher.none.fl_str_mv |
Institute for Medical Research and Occupational Health |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
instname_str |
Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
instacron_str |
RCAAP |
institution |
RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
collection |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository.name.fl_str_mv |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
repository.mail.fl_str_mv |
|
_version_ |
1799132119048388608 |