Assessment of exposure to the Penicillium glabrum complex in cork industry using complementing methods

Detalhes bibliográficos
Autor(a) principal: Viegas, C.
Data de Publicação: 2015
Outros Autores: Sabino, Raquel, Botelho, D., Santos, M., Gomes, A.Q.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10400.18/3508
Resumo: Cork oak is the second most dominant forest species in Portugal and makes this country the world leader in cork export. Occupational exposure to Chrysonilia sitophila and the Penicillium glabrum complex in cork industry is common, and the latter fungus is associated with suberosis. However, as conventional methods seem to underestimate its presence in occupational environments, the aim of our study was to see whether information obtained by polymerase chain reaction (PCR), a molecular-based method, can complement conventional findings and give a better insight into occupational exposure of cork industry workers. We assessed fungal contamination with the P. glabrum complex in three cork manufacturing plants in the outskirts of Lisbon using both conventional and molecular methods. Conventional culturing failed to detect the fungus at six sampling sites in which PCR did detect it. This confirms our assumption that the use of complementing methods can provide information for a more accurate assessment of occupational exposure to the P. glabrum complex in cork industry.
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spelling Assessment of exposure to the Penicillium glabrum complex in cork industry using complementing methodsOcjena izloženosti kompleksu Penicillium glabrum u proizvodnji pluta s pomoću komplementarnih metodaCork OakOccupational ExposurePenicillium glabrumUngal ExposureMolecular MethodsPCRPortugalSuberosisInfecções Sistémicas e ZoonosesCork oak is the second most dominant forest species in Portugal and makes this country the world leader in cork export. Occupational exposure to Chrysonilia sitophila and the Penicillium glabrum complex in cork industry is common, and the latter fungus is associated with suberosis. However, as conventional methods seem to underestimate its presence in occupational environments, the aim of our study was to see whether information obtained by polymerase chain reaction (PCR), a molecular-based method, can complement conventional findings and give a better insight into occupational exposure of cork industry workers. We assessed fungal contamination with the P. glabrum complex in three cork manufacturing plants in the outskirts of Lisbon using both conventional and molecular methods. Conventional culturing failed to detect the fungus at six sampling sites in which PCR did detect it. This confirms our assumption that the use of complementing methods can provide information for a more accurate assessment of occupational exposure to the P. glabrum complex in cork industry.Institute for Medical Research and Occupational HealthRepositório Científico do Instituto Nacional de SaúdeViegas, C.Sabino, RaquelBotelho, D.Santos, M.Gomes, A.Q.2016-02-26T16:40:41Z2015-09-012015-09-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10400.18/3508engArh Hig Rada Toksikol. 2015 Sep 1;66(3):203-7. doi: 10.1515/aiht-2015-66-26140004-125410.1515/aiht-2015-66-2614info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-20T15:39:46Zoai:repositorio.insa.pt:10400.18/3508Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T18:38:16.762300Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Assessment of exposure to the Penicillium glabrum complex in cork industry using complementing methods
Ocjena izloženosti kompleksu Penicillium glabrum u proizvodnji pluta s pomoću komplementarnih metoda
title Assessment of exposure to the Penicillium glabrum complex in cork industry using complementing methods
spellingShingle Assessment of exposure to the Penicillium glabrum complex in cork industry using complementing methods
Viegas, C.
Cork Oak
Occupational Exposure
Penicillium glabrum
Ungal Exposure
Molecular Methods
PCR
Portugal
Suberosis
Infecções Sistémicas e Zoonoses
title_short Assessment of exposure to the Penicillium glabrum complex in cork industry using complementing methods
title_full Assessment of exposure to the Penicillium glabrum complex in cork industry using complementing methods
title_fullStr Assessment of exposure to the Penicillium glabrum complex in cork industry using complementing methods
title_full_unstemmed Assessment of exposure to the Penicillium glabrum complex in cork industry using complementing methods
title_sort Assessment of exposure to the Penicillium glabrum complex in cork industry using complementing methods
author Viegas, C.
author_facet Viegas, C.
Sabino, Raquel
Botelho, D.
Santos, M.
Gomes, A.Q.
author_role author
author2 Sabino, Raquel
Botelho, D.
Santos, M.
Gomes, A.Q.
author2_role author
author
author
author
dc.contributor.none.fl_str_mv Repositório Científico do Instituto Nacional de Saúde
dc.contributor.author.fl_str_mv Viegas, C.
Sabino, Raquel
Botelho, D.
Santos, M.
Gomes, A.Q.
dc.subject.por.fl_str_mv Cork Oak
Occupational Exposure
Penicillium glabrum
Ungal Exposure
Molecular Methods
PCR
Portugal
Suberosis
Infecções Sistémicas e Zoonoses
topic Cork Oak
Occupational Exposure
Penicillium glabrum
Ungal Exposure
Molecular Methods
PCR
Portugal
Suberosis
Infecções Sistémicas e Zoonoses
description Cork oak is the second most dominant forest species in Portugal and makes this country the world leader in cork export. Occupational exposure to Chrysonilia sitophila and the Penicillium glabrum complex in cork industry is common, and the latter fungus is associated with suberosis. However, as conventional methods seem to underestimate its presence in occupational environments, the aim of our study was to see whether information obtained by polymerase chain reaction (PCR), a molecular-based method, can complement conventional findings and give a better insight into occupational exposure of cork industry workers. We assessed fungal contamination with the P. glabrum complex in three cork manufacturing plants in the outskirts of Lisbon using both conventional and molecular methods. Conventional culturing failed to detect the fungus at six sampling sites in which PCR did detect it. This confirms our assumption that the use of complementing methods can provide information for a more accurate assessment of occupational exposure to the P. glabrum complex in cork industry.
publishDate 2015
dc.date.none.fl_str_mv 2015-09-01
2015-09-01T00:00:00Z
2016-02-26T16:40:41Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10400.18/3508
url http://hdl.handle.net/10400.18/3508
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Arh Hig Rada Toksikol. 2015 Sep 1;66(3):203-7. doi: 10.1515/aiht-2015-66-2614
0004-1254
10.1515/aiht-2015-66-2614
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Institute for Medical Research and Occupational Health
publisher.none.fl_str_mv Institute for Medical Research and Occupational Health
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
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collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
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