Heterologous expression of nifA or nodD genes improves chickpea-Mesorhizobium symbiotic performance

Detalhes bibliográficos
Autor(a) principal: da-Silva, J. R.
Data de Publicação: 2019
Outros Autores: Menéndez, E., Eliziário, F., Mateos, P. F., Alexandre, A., Oliveira, S.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10174/27939
https://doi.org/da-Silva, J.R., Menéndez, E., Eliziário, F. et al. Heterologous expression of nifA or nodD genes improves chickpea-Mesorhizobium symbiotic performance. Plant Soil 436, 607–621 (2019). https://doi.org/10.1007/s11104-019-03950-0
https://doi.org/10.1007/s11104-019-03950-0
Resumo: Aims The aim of this study was to investigate whether the overexpression of NifA and NodD regulators contribute to the symbiotic improvement of chickpea mesorhizobia. Methods The native strains V-15b, ST-2, and PMI-6 were transformed with extra copies of nifA or nodD genes and several plants trial were performed. Results Plant growth assays showed that nifA overexpression was able to improve the symbiotic effectiveness of V-15b, while nodD overexpression lead to the improvement of ST-2 and PMI-6. Hydroponic assays showed that plants inoculated with V15bnifA+ and PMI6nodD+ started developing nodules earlier than those inoculated with the corresponding control strains. In addition, the number of nodules was always higher in plants inoculated with the strains overexpressing the symbiotic genes. Analysis of histological sections of nodules formed by V15bnifA+ showed a more developed fixation zone when compared with control. On the other hand, nodules induced by PMI6nodD+ did not show a senescent zone, which was observed in nodules from plants inoculated with the control strain. Plants inoculated with PMI6nodD+ and ST2nodD+ showed a higher number of infection threads than the corresponding control inoculations. Conclusion These results indicate that overexpressing nifA and nodD may be an important tool to achieve the improvement of the symbiotic performance of mesorhizobia.
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spelling Heterologous expression of nifA or nodD genes improves chickpea-Mesorhizobium symbiotic performancenitrogen fixationrhizobiasymbiotic effectivenesslegumenodulationoverexpressionAims The aim of this study was to investigate whether the overexpression of NifA and NodD regulators contribute to the symbiotic improvement of chickpea mesorhizobia. Methods The native strains V-15b, ST-2, and PMI-6 were transformed with extra copies of nifA or nodD genes and several plants trial were performed. Results Plant growth assays showed that nifA overexpression was able to improve the symbiotic effectiveness of V-15b, while nodD overexpression lead to the improvement of ST-2 and PMI-6. Hydroponic assays showed that plants inoculated with V15bnifA+ and PMI6nodD+ started developing nodules earlier than those inoculated with the corresponding control strains. In addition, the number of nodules was always higher in plants inoculated with the strains overexpressing the symbiotic genes. Analysis of histological sections of nodules formed by V15bnifA+ showed a more developed fixation zone when compared with control. On the other hand, nodules induced by PMI6nodD+ did not show a senescent zone, which was observed in nodules from plants inoculated with the control strain. Plants inoculated with PMI6nodD+ and ST2nodD+ showed a higher number of infection threads than the corresponding control inoculations. Conclusion These results indicate that overexpressing nifA and nodD may be an important tool to achieve the improvement of the symbiotic performance of mesorhizobia.The authors thank Dr Alvaro Peix (IRNASA-CSIC) for providing pMP4661 plasmid. Moreover, the authors thank G. Mariano for technical assistance. This work was financed by FEDER Funds through the Operational Program for Competitiveness Factors - COMPETE and National Funds through FCT (Fundação para a Ciência e a Tecnologia), under the Strategic Project UID/AGR/00115/2013, Project nº FCOMP-01-0124-FEDER-028316 (PTDC/BIA-EVF/4158/2012), project POCI-01-0145-FEDER-016810 (PTDC/AGR-PRO/2978/2014) and InAlentejo ALENT-07-0262-FEDER-001871. J. Rodrigo da-Silva acknowledges a PhD fellowship (1254-13-8) from CAPES (Coordenação de Aperfeiçoamento de Pessoal de Nível Superior).Plant and Soil, Springer Nature2020-07-06T10:32:13Z2020-07-062019-02-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://hdl.handle.net/10174/27939https://doi.org/da-Silva, J.R., Menéndez, E., Eliziário, F. et al. Heterologous expression of nifA or nodD genes improves chickpea-Mesorhizobium symbiotic performance. Plant Soil 436, 607–621 (2019). https://doi.org/10.1007/s11104-019-03950-0https://doi.org/10.1007/s11104-019-03950-0http://hdl.handle.net/10174/27939https://doi.org/10.1007/s11104-019-03950-0engjrsilva@uevora.ptesthermenendez@uevora.ptfernando.eliziario@gmail.compfmg@usal.esanaalex@uevora.ptnd227da-Silva, J. R.Menéndez, E.Eliziário, F.Mateos, P. F.Alexandre, A.Oliveira, S.info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-01-03T19:23:39Zoai:dspace.uevora.pt:10174/27939Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T01:17:50.800280Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Heterologous expression of nifA or nodD genes improves chickpea-Mesorhizobium symbiotic performance
title Heterologous expression of nifA or nodD genes improves chickpea-Mesorhizobium symbiotic performance
spellingShingle Heterologous expression of nifA or nodD genes improves chickpea-Mesorhizobium symbiotic performance
da-Silva, J. R.
nitrogen fixation
rhizobia
symbiotic effectiveness
legume
nodulation
overexpression
title_short Heterologous expression of nifA or nodD genes improves chickpea-Mesorhizobium symbiotic performance
title_full Heterologous expression of nifA or nodD genes improves chickpea-Mesorhizobium symbiotic performance
title_fullStr Heterologous expression of nifA or nodD genes improves chickpea-Mesorhizobium symbiotic performance
title_full_unstemmed Heterologous expression of nifA or nodD genes improves chickpea-Mesorhizobium symbiotic performance
title_sort Heterologous expression of nifA or nodD genes improves chickpea-Mesorhizobium symbiotic performance
author da-Silva, J. R.
author_facet da-Silva, J. R.
Menéndez, E.
Eliziário, F.
Mateos, P. F.
Alexandre, A.
Oliveira, S.
author_role author
author2 Menéndez, E.
Eliziário, F.
Mateos, P. F.
Alexandre, A.
Oliveira, S.
author2_role author
author
author
author
author
dc.contributor.author.fl_str_mv da-Silva, J. R.
Menéndez, E.
Eliziário, F.
Mateos, P. F.
Alexandre, A.
Oliveira, S.
dc.subject.por.fl_str_mv nitrogen fixation
rhizobia
symbiotic effectiveness
legume
nodulation
overexpression
topic nitrogen fixation
rhizobia
symbiotic effectiveness
legume
nodulation
overexpression
description Aims The aim of this study was to investigate whether the overexpression of NifA and NodD regulators contribute to the symbiotic improvement of chickpea mesorhizobia. Methods The native strains V-15b, ST-2, and PMI-6 were transformed with extra copies of nifA or nodD genes and several plants trial were performed. Results Plant growth assays showed that nifA overexpression was able to improve the symbiotic effectiveness of V-15b, while nodD overexpression lead to the improvement of ST-2 and PMI-6. Hydroponic assays showed that plants inoculated with V15bnifA+ and PMI6nodD+ started developing nodules earlier than those inoculated with the corresponding control strains. In addition, the number of nodules was always higher in plants inoculated with the strains overexpressing the symbiotic genes. Analysis of histological sections of nodules formed by V15bnifA+ showed a more developed fixation zone when compared with control. On the other hand, nodules induced by PMI6nodD+ did not show a senescent zone, which was observed in nodules from plants inoculated with the control strain. Plants inoculated with PMI6nodD+ and ST2nodD+ showed a higher number of infection threads than the corresponding control inoculations. Conclusion These results indicate that overexpressing nifA and nodD may be an important tool to achieve the improvement of the symbiotic performance of mesorhizobia.
publishDate 2019
dc.date.none.fl_str_mv 2019-02-01T00:00:00Z
2020-07-06T10:32:13Z
2020-07-06
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10174/27939
https://doi.org/da-Silva, J.R., Menéndez, E., Eliziário, F. et al. Heterologous expression of nifA or nodD genes improves chickpea-Mesorhizobium symbiotic performance. Plant Soil 436, 607–621 (2019). https://doi.org/10.1007/s11104-019-03950-0
https://doi.org/10.1007/s11104-019-03950-0
http://hdl.handle.net/10174/27939
https://doi.org/10.1007/s11104-019-03950-0
url http://hdl.handle.net/10174/27939
https://doi.org/da-Silva, J.R., Menéndez, E., Eliziário, F. et al. Heterologous expression of nifA or nodD genes improves chickpea-Mesorhizobium symbiotic performance. Plant Soil 436, 607–621 (2019). https://doi.org/10.1007/s11104-019-03950-0
https://doi.org/10.1007/s11104-019-03950-0
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv jrsilva@uevora.pt
esthermenendez@uevora.pt
fernando.eliziario@gmail.com
pfmg@usal.es
anaalex@uevora.pt
nd
227
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Plant and Soil, Springer Nature
publisher.none.fl_str_mv Plant and Soil, Springer Nature
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
repository.mail.fl_str_mv
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