Genetic diversity and symbiotic compatibility among rhizobial strains and Desmodium incanum and Lotus spp. plants
Autor(a) principal: | |
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Data de Publicação: | 2014 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Genetics and Molecular Biology |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572014000300012 |
Resumo: | This work aimed to evaluate the symbiotic compatibility and nodulation efficiency of rhizobia isolated from Desmodium incanum, Lotus corniculatus, L. subbiflorus, L. uliginosus and L. glaber plants by cross-inoculation. Twelve reference strains and 21 native isolates of rhizobia were genetically analyzed by the BOX-PCR technique, which showed a high genetic diversity among the rhizobia studied. The isolates were also characterized based on their production of indolic compounds and siderophores, as well as on their tolerance to salinity. Fifteen of the 33 rhizobia analyzed were able to produce indolic compounds, whereas 13 produced siderophores. All the tested rhizobia were sensitive to high salinity, although some were able to grow in solutions of up to 2% NaCl. Most of the native rhizobia isolated from L. uliginosus were able to induce nodulation in all plant species studied. In a greenhouse experiment using both D. incanum and L. corniculatus plants, the rhizobia isolate UFRGS Lu2 promoted the greatest plant growth. The results demonstrate that there are native rhizobia in the soils of southern Brazil that have low host specificity and are able to induce nodulation and form active nodules in several plant species. |
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Genetic diversity and symbiotic compatibility among rhizobial strains and Desmodium incanum and Lotus spp. plantsRhizobianodulationnitrogenspecificityThis work aimed to evaluate the symbiotic compatibility and nodulation efficiency of rhizobia isolated from Desmodium incanum, Lotus corniculatus, L. subbiflorus, L. uliginosus and L. glaber plants by cross-inoculation. Twelve reference strains and 21 native isolates of rhizobia were genetically analyzed by the BOX-PCR technique, which showed a high genetic diversity among the rhizobia studied. The isolates were also characterized based on their production of indolic compounds and siderophores, as well as on their tolerance to salinity. Fifteen of the 33 rhizobia analyzed were able to produce indolic compounds, whereas 13 produced siderophores. All the tested rhizobia were sensitive to high salinity, although some were able to grow in solutions of up to 2% NaCl. Most of the native rhizobia isolated from L. uliginosus were able to induce nodulation in all plant species studied. In a greenhouse experiment using both D. incanum and L. corniculatus plants, the rhizobia isolate UFRGS Lu2 promoted the greatest plant growth. The results demonstrate that there are native rhizobia in the soils of southern Brazil that have low host specificity and are able to induce nodulation and form active nodules in several plant species.Sociedade Brasileira de Genética2014-06-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572014000300012Genetics and Molecular Biology v.37 n.2 2014reponame:Genetics and Molecular Biologyinstname:Sociedade Brasileira de Genética (SBG)instacron:SBG10.1590/S1415-47572014000300012info:eu-repo/semantics/openAccessGranada,Camille EStrochein,MarcosVargas,Luciano KBruxel,ManuelaSá,Enilson Luiz Saccol dePassaglia,Luciane M.Peng2014-09-17T00:00:00Zoai:scielo:S1415-47572014000300012Revistahttp://www.gmb.org.br/ONGhttps://old.scielo.br/oai/scielo-oai.php||editor@gmb.org.br1678-46851415-4757opendoar:2014-09-17T00:00Genetics and Molecular Biology - Sociedade Brasileira de Genética (SBG)false |
dc.title.none.fl_str_mv |
Genetic diversity and symbiotic compatibility among rhizobial strains and Desmodium incanum and Lotus spp. plants |
title |
Genetic diversity and symbiotic compatibility among rhizobial strains and Desmodium incanum and Lotus spp. plants |
spellingShingle |
Genetic diversity and symbiotic compatibility among rhizobial strains and Desmodium incanum and Lotus spp. plants Granada,Camille E Rhizobia nodulation nitrogen specificity |
title_short |
Genetic diversity and symbiotic compatibility among rhizobial strains and Desmodium incanum and Lotus spp. plants |
title_full |
Genetic diversity and symbiotic compatibility among rhizobial strains and Desmodium incanum and Lotus spp. plants |
title_fullStr |
Genetic diversity and symbiotic compatibility among rhizobial strains and Desmodium incanum and Lotus spp. plants |
title_full_unstemmed |
Genetic diversity and symbiotic compatibility among rhizobial strains and Desmodium incanum and Lotus spp. plants |
title_sort |
Genetic diversity and symbiotic compatibility among rhizobial strains and Desmodium incanum and Lotus spp. plants |
author |
Granada,Camille E |
author_facet |
Granada,Camille E Strochein,Marcos Vargas,Luciano K Bruxel,Manuela Sá,Enilson Luiz Saccol de Passaglia,Luciane M.P |
author_role |
author |
author2 |
Strochein,Marcos Vargas,Luciano K Bruxel,Manuela Sá,Enilson Luiz Saccol de Passaglia,Luciane M.P |
author2_role |
author author author author author |
dc.contributor.author.fl_str_mv |
Granada,Camille E Strochein,Marcos Vargas,Luciano K Bruxel,Manuela Sá,Enilson Luiz Saccol de Passaglia,Luciane M.P |
dc.subject.por.fl_str_mv |
Rhizobia nodulation nitrogen specificity |
topic |
Rhizobia nodulation nitrogen specificity |
description |
This work aimed to evaluate the symbiotic compatibility and nodulation efficiency of rhizobia isolated from Desmodium incanum, Lotus corniculatus, L. subbiflorus, L. uliginosus and L. glaber plants by cross-inoculation. Twelve reference strains and 21 native isolates of rhizobia were genetically analyzed by the BOX-PCR technique, which showed a high genetic diversity among the rhizobia studied. The isolates were also characterized based on their production of indolic compounds and siderophores, as well as on their tolerance to salinity. Fifteen of the 33 rhizobia analyzed were able to produce indolic compounds, whereas 13 produced siderophores. All the tested rhizobia were sensitive to high salinity, although some were able to grow in solutions of up to 2% NaCl. Most of the native rhizobia isolated from L. uliginosus were able to induce nodulation in all plant species studied. In a greenhouse experiment using both D. incanum and L. corniculatus plants, the rhizobia isolate UFRGS Lu2 promoted the greatest plant growth. The results demonstrate that there are native rhizobia in the soils of southern Brazil that have low host specificity and are able to induce nodulation and form active nodules in several plant species. |
publishDate |
2014 |
dc.date.none.fl_str_mv |
2014-06-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572014000300012 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572014000300012 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/S1415-47572014000300012 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Sociedade Brasileira de Genética |
publisher.none.fl_str_mv |
Sociedade Brasileira de Genética |
dc.source.none.fl_str_mv |
Genetics and Molecular Biology v.37 n.2 2014 reponame:Genetics and Molecular Biology instname:Sociedade Brasileira de Genética (SBG) instacron:SBG |
instname_str |
Sociedade Brasileira de Genética (SBG) |
instacron_str |
SBG |
institution |
SBG |
reponame_str |
Genetics and Molecular Biology |
collection |
Genetics and Molecular Biology |
repository.name.fl_str_mv |
Genetics and Molecular Biology - Sociedade Brasileira de Genética (SBG) |
repository.mail.fl_str_mv |
||editor@gmb.org.br |
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1752122385891328000 |