An efficient chronic unpredictable stress protocol to induce stress-relatec responses in C57BL/6 mice

Detalhes bibliográficos
Autor(a) principal: Monteiro, S.
Data de Publicação: 2015
Outros Autores: Roque, Susana, Calçada, Daniela Alexandra Sá, Sousa, Nuno, Neves, Margarida Correia, Cerqueira, João José
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/1822/40051
Resumo: Exposure to chronic stress can have broad effects on health ranging from increased predisposition for neuropsychiatric disorders to deregulation of immune responses. The chronic unpredictable stress (CUS) protocol has been widely used to study the impact of stress exposure in several animal models and consists in the random, intermittent, and unpredictable exposure to a variety of stressors during several weeks. CUS has consistently been shown to induce behavioral and immunological alterations typical of the chronic stress-response. Unfortunately C57BL/6 mice, one of the most widely used mouse strains, due to the great variety of genetically modified lines, seem to be resistant to the commonly used 4-week-long CUS protocol. The definition of an alternative CUS protocol allowing the use of C57BL/6 mice in chronic stress experiments is a need. Here, we show that by extending the CUS protocol to 8?weeks is possible to induce a chronic stress-response in C57BL/6 mice, as revealed by abrogated body weight gain, increased adrenals weight, and an overactive hypothalamic-pituitary-adrenal axis with increased levels of serum corticosterone. Moreover, we also observed stress-associated behavioral alterations, including the potentiation of anxious-like and depressive-like behaviors and a reduction of exploratory behavior, as well as subtle stress-related changes in the cell population of the thymus and of the spleen. The present protocol for C57BL/6 mice consistently triggers the spectrum of CUS-induced changes observed in rats and, thus, will be highly useful to researchers that need to use this particular mouse strain as an animal model of neuropsychiatric disorders and/or immune deregulation related to CUS.
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spelling An efficient chronic unpredictable stress protocol to induce stress-relatec responses in C57BL/6 miceChronic stressCUSNeuropsychiatric disordersImmune dysfunctionAnxietyDepressive-like behaviorSocial defeatScience & TechnologyExposure to chronic stress can have broad effects on health ranging from increased predisposition for neuropsychiatric disorders to deregulation of immune responses. The chronic unpredictable stress (CUS) protocol has been widely used to study the impact of stress exposure in several animal models and consists in the random, intermittent, and unpredictable exposure to a variety of stressors during several weeks. CUS has consistently been shown to induce behavioral and immunological alterations typical of the chronic stress-response. Unfortunately C57BL/6 mice, one of the most widely used mouse strains, due to the great variety of genetically modified lines, seem to be resistant to the commonly used 4-week-long CUS protocol. The definition of an alternative CUS protocol allowing the use of C57BL/6 mice in chronic stress experiments is a need. Here, we show that by extending the CUS protocol to 8?weeks is possible to induce a chronic stress-response in C57BL/6 mice, as revealed by abrogated body weight gain, increased adrenals weight, and an overactive hypothalamic-pituitary-adrenal axis with increased levels of serum corticosterone. Moreover, we also observed stress-associated behavioral alterations, including the potentiation of anxious-like and depressive-like behaviors and a reduction of exploratory behavior, as well as subtle stress-related changes in the cell population of the thymus and of the spleen. The present protocol for C57BL/6 mice consistently triggers the spectrum of CUS-induced changes observed in rats and, thus, will be highly useful to researchers that need to use this particular mouse strain as an animal model of neuropsychiatric disorders and/or immune deregulation related to CUS.(undefined)Frontiers MediaUniversidade do MinhoMonteiro, S.Roque, SusanaCalçada, Daniela Alexandra SáSousa, NunoNeves, Margarida CorreiaCerqueira, João José20152015-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/1822/40051engMonteiro, S., Roque, S., de Sá-Calçada, D., Sousa, N., Correia-Neves, M., & Cerqueira, J. J. (2015). An efficient chronic unpredictable stress protocol to induce stress-related responses in C57BL/6 mice. Frontiers in Psychiatry, 6(FEB). doi: 10.3389/fpsyt.2015.000061664-064010.3389/fpsyt.2015.00006http://journal.frontiersin.org/journal/psychiatryinfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-21T12:39:48Zoai:repositorium.sdum.uminho.pt:1822/40051Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T19:36:28.162216Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv An efficient chronic unpredictable stress protocol to induce stress-relatec responses in C57BL/6 mice
title An efficient chronic unpredictable stress protocol to induce stress-relatec responses in C57BL/6 mice
spellingShingle An efficient chronic unpredictable stress protocol to induce stress-relatec responses in C57BL/6 mice
Monteiro, S.
Chronic stress
CUS
Neuropsychiatric disorders
Immune dysfunction
Anxiety
Depressive-like behavior
Social defeat
Science & Technology
title_short An efficient chronic unpredictable stress protocol to induce stress-relatec responses in C57BL/6 mice
title_full An efficient chronic unpredictable stress protocol to induce stress-relatec responses in C57BL/6 mice
title_fullStr An efficient chronic unpredictable stress protocol to induce stress-relatec responses in C57BL/6 mice
title_full_unstemmed An efficient chronic unpredictable stress protocol to induce stress-relatec responses in C57BL/6 mice
title_sort An efficient chronic unpredictable stress protocol to induce stress-relatec responses in C57BL/6 mice
author Monteiro, S.
author_facet Monteiro, S.
Roque, Susana
Calçada, Daniela Alexandra Sá
Sousa, Nuno
Neves, Margarida Correia
Cerqueira, João José
author_role author
author2 Roque, Susana
Calçada, Daniela Alexandra Sá
Sousa, Nuno
Neves, Margarida Correia
Cerqueira, João José
author2_role author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade do Minho
dc.contributor.author.fl_str_mv Monteiro, S.
Roque, Susana
Calçada, Daniela Alexandra Sá
Sousa, Nuno
Neves, Margarida Correia
Cerqueira, João José
dc.subject.por.fl_str_mv Chronic stress
CUS
Neuropsychiatric disorders
Immune dysfunction
Anxiety
Depressive-like behavior
Social defeat
Science & Technology
topic Chronic stress
CUS
Neuropsychiatric disorders
Immune dysfunction
Anxiety
Depressive-like behavior
Social defeat
Science & Technology
description Exposure to chronic stress can have broad effects on health ranging from increased predisposition for neuropsychiatric disorders to deregulation of immune responses. The chronic unpredictable stress (CUS) protocol has been widely used to study the impact of stress exposure in several animal models and consists in the random, intermittent, and unpredictable exposure to a variety of stressors during several weeks. CUS has consistently been shown to induce behavioral and immunological alterations typical of the chronic stress-response. Unfortunately C57BL/6 mice, one of the most widely used mouse strains, due to the great variety of genetically modified lines, seem to be resistant to the commonly used 4-week-long CUS protocol. The definition of an alternative CUS protocol allowing the use of C57BL/6 mice in chronic stress experiments is a need. Here, we show that by extending the CUS protocol to 8?weeks is possible to induce a chronic stress-response in C57BL/6 mice, as revealed by abrogated body weight gain, increased adrenals weight, and an overactive hypothalamic-pituitary-adrenal axis with increased levels of serum corticosterone. Moreover, we also observed stress-associated behavioral alterations, including the potentiation of anxious-like and depressive-like behaviors and a reduction of exploratory behavior, as well as subtle stress-related changes in the cell population of the thymus and of the spleen. The present protocol for C57BL/6 mice consistently triggers the spectrum of CUS-induced changes observed in rats and, thus, will be highly useful to researchers that need to use this particular mouse strain as an animal model of neuropsychiatric disorders and/or immune deregulation related to CUS.
publishDate 2015
dc.date.none.fl_str_mv 2015
2015-01-01T00:00:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/1822/40051
url http://hdl.handle.net/1822/40051
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Monteiro, S., Roque, S., de Sá-Calçada, D., Sousa, N., Correia-Neves, M., & Cerqueira, J. J. (2015). An efficient chronic unpredictable stress protocol to induce stress-related responses in C57BL/6 mice. Frontiers in Psychiatry, 6(FEB). doi: 10.3389/fpsyt.2015.00006
1664-0640
10.3389/fpsyt.2015.00006
http://journal.frontiersin.org/journal/psychiatry
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Frontiers Media
publisher.none.fl_str_mv Frontiers Media
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
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